Abstract
The cross-linked hydrogel films containing sodium fucidate were previously reported to be prepared polyvinyl alcohol (PVA) and sodium carboxymethylcellulose (Na-CMC) using the freeze-thawing method and their physicochemical property was investigated. For the development of novel sodium fucidate-loaded wound dressing, here its in vivo wound healing test and histopathology were performed compared with the conventional ointment product. In wound healing test, the sodium fucidate-loaded composed of 2.5% PVA, 1.125% Na-CMC and 0.2% drug showed faster healing of the wound made in rat dorsum than the hydrogel without drug, indicating the potential healing effect of sodium fucidate. Furthermore, from the histological examination, the healing effect of sodium fucidate-loaded hydrogel was greater than that of the conventional ointment product and hydrogel without drug, since it might gave an adequate level of moisture and build up the exudates on the wound area. Thus, the sodium fucidate-loaded wound dressing composed of 5% PVA, 1.125% Na-CMC and 0.2% drug is a potential wound dressing with excellent wound healing.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ajji, Z., Mirjalili, G., Alkhatab, A., and Dada, H., Use of electron beam for the production of hydrogel dressings. Radiat. Phys. Chem., 77, 200–202 (2008).
Balakrishnan, B., Mohanty, M., Umashankar, P. R., and Jayakrishnan, A., Evaluation of an in situ forming hydrogel wound dressing based on oxidised alginate and gelatin. Biomaterials, 26, 6335–6342 (2005).
Cascone, M. G., Sim, B., and Downes, S., Blends of synthetic and natural polymers as drug delivery systems for growth hormone. Biomaterials, 16, 569–574 (1995).
Dennis, S., Fucidic acid in skin and soft tissue infections. Int. J. Antimi. Ag., 12, S59–S66 (1999).
Evans, P., The healing process at cellular level: a review. Physiotherapy, 66, 256–259 (1980).
Gaisford, S., Beezer, A. E., Bishop, A. H., Walker, M., and Parsons, D., An in vitro method for the quantitative determination of the antimicrobial efficacy of silver-containing wound dressings. Int. J. Pharm., 366, 111–116 (2009).
Halper, J., Leshin, L. S., Lewis, S. J., and Li, W. I., Wound healing and angiogenic properties of supernatants from Lactobacillus cultures. Exp. Biol. Med. (Maywood), 228, 1329–1337 (2003).
Hinnman, C. D. and Maibach. H., Effect of air exposure and occlusion on experimental human skin wounds. Nature, 200, 377–378 (1963).
Hirose, K., Onishi, H., Sasatsu, M., Takeshita, K., Kouzuma, K., Isowa, K., and Machida, Y., In vivo evaluation of Kumazasa extract and Na-CMC films containing the extract against deep skin ulcer model in rats. Biol. Pharm. Bull., 30, 2406–2411 (2007).
Hoffman, A. S., Hydrogels for biomedical applications. Adv. Drug Deliv. Rev., 54, 3–12 (2002).
Kim, H. J., Choi, E. Y., Oh, J. S., Lee, H. C., Park, S. S., and Cho, C. S., Possibility of wound dressing using poly (L-leucine)/poly(ethylene glycol)/poly(L-leucine)triblock copolymer. Biomaterials, 21, 131–141 (2000).
Kim, I. Y., Yoo, M.K., Kim, B. C., Kim. S. K., Lee, H. C., and Cho, C. S., Preparation of semi-interpenetrating polymer networks composed of Na-CMC and poloxamer. Int. J. Biol. Macromol., 38, 51–58 (2006).
Kim, J. O., Park, J. K., Kim, J. H., Jin, S. G., Yong, C. S., Li, D. X., Choi, J. Y., Woo, J. S., Yoo, B. K., Lyoo, W. S., Kim, J. A., and Choi, H. G., Development of polyvinyl alcohol-sodium alginate gel-matrix-based wound dressing system containing nitrofurazone. Int. J. Pharm., 359, 79–86 (2008a).
Kim, J. O., Choi, J. Y., Park, J. K., Kim, J. H., Jin, S. G., Chang, S. W., Li, D. X., Hwang, M. R., Woo, J. S., Kim, J. A., Lyoo, W. S., Yong, C. S., and Choi, H. G., Development of clindamycin-loaded wound dressing with polyvinyl alcohol and sodium alginate. Biol. Pharm. Bull., 31, 2277–2282 (2008b).
Martin, P., Wound healing-aiming for perfect skin regeneration. Science, 276, 75–81 (1997).
Morin, R. J. and Tomaselli, N. L., Interactive dressings and topical agents. Clin. Plast. Surg., 34, 643–658 (2007).
Queen, D., Gaylor, J. D. S., Evans, J. H., Courtney, J. M., and Reid, W. H., The preclinical evaluation of the water vapor transmission rate through burn wound dressings. Biomaterials, 8, 367–371 (1987).
Quintanilha Ribeiro, F. A., Borges, J. P., Guaraldo, L., and Vianna, M. R., Study of wound healing in rats treated with topical and injected mitomycin-C. Braz. J. Otorhinolaryngol., 74, 328–330 (2008).
Society of Toxicology (SOT), Guilding Priciples in the Use of Animals in Toxicology: www.toxicology.org/AI/FA/guidingprinciples.pdf (1999).
Stashak, T. S., Farstvedt, E., and Othic, A., Update on wound dressings: Indications and best use. Clin. Tec. Eq. Prac., 3, 148–163 (2004).
Tabbara, K. F., Antonios, S., and Alvarez, H., Effects of fucidic acid on staphylococcal keratitis. Br. J. Ophthalmol., 73, 136–139 (1989).
Turner, T. D., Hospital usage of absorbent dressings. Pharm. J., 222, 421–424 (1979).
Valenta, C. and Auner, B. G., The use of polymers for dermal and transdermal delivery. Eur. J. Pharm. Biopharm., 58, 279–289 (2004).
Winter, G. D., Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig. Nature, 193, 293–294 (1962).
Yeo, J. H., Lee, K. G., Kim, H. C., Oh, Y. L., Kim, A., J. and Kim, S. Y., The effects of PVA/Na-CMC/fibroin (PCF)-blended spongy sheets on wound healing in rats. Biol. Pharm. Bull., 23, 1220–1223 (2000).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Lee, J.H., Lim, SJ., Oh, D.H. et al. Wound healing evaluation of sodium fucidate-loaded polyvinylalcohol/sodium carboxymethylcellulose-based wound dressing. Arch. Pharm. Res. 33, 1083–1089 (2010). https://doi.org/10.1007/s12272-010-0715-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-010-0715-2