Abstract
We examined anti-amnesic activity of the methanolic extract of Cornus officinalis fruits (COT) and a major constituent, loganin using scopolamine-induced (1 mg/kg body weight, s.c.) amnesic mice with both passive avoidance and the Morris water maze tests. Oral treatment of mice with COT (100 mg/kg body weight) and loganin (1 and 2 mg/kg body weight) significantly mitigated scopolamine-induced memory deficits in passive avoidance test. In the Morris water maze test, oral treatment of loganin significantly ameliorated scopolamine-induced memory deficits showing the formation of long-term and/or short-term spatial memory. Moreover, loganin (2 mg/kg body weight) significantly inhibited acetylcholinesterase activity by as much as 45% of control in the mouse hippocampus. These results indicate that loganin may exert antiamnesic activity in in vivo through acetylcholinesterase inhibition.
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Bartus, R. T., Dean, R. L. III., Beer, B., and Lippa, A. S., The cholinergic hypothesis of geriatric memory dysfunction. Science, 217, 408–414 (1982).
Bejar, C., Wang, R. H., and Weinstock, M., Effect of rivastigmine on scopolamine-induced memory impairment in rats. Eur. J. Pharmacol., 383, 231–240 (1999).
Collerton, D., Cholinergic function and intellectual decline in Alzheimer’s disease. Neuroscience, 19, 1–28 (1986).
Ellman, G. L., Courtney, K. D., Andre, V. Jr., and Featherstone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 7, 88–95 (1961).
Francis, P. T., Webster, M. T., Chessell, I. P., Holmes, C., Stratmann, G. C., Procter, A. W., Cross, A. J., Green, A.R., and Bowen, D. M., Neurotransmitters and second messengers in aging and Alzheimer’s disease. Ann. NY Acad. Sci., 695, 19 (1993).
Guo, L. L., Zhou, Y., Wang, X. D., Zhang, L., Zhao, S. P., and Ge, D. Y., Effects of the extract of the fruit of Cornus officinalis on rat splenic lymphocytic proliferation. Chin. J. Beijing Univ. TCM, 24, 38–40 (2001).
Kang, S. Y., Lee, K. Y., Park, M. J., Kim, Y. C., Markelonis, G. J., Oh, T. H., and Kim, Y. C., Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol. Learn. Mem., 79, 11–18 (2003).
Kim, D. K. and Kwak, J. H., A furan derivative from Cornus officinalis. Arch. Pharm. Res., 21, 787–789 (1998).
Kim, S. R., Hwang, S. Y., Jang, Y. P., Park, M. J., Markelonis, G. J., Oh, T. H., and Kim, Y. C., Protopine from Corydalis ternata has anticholinesterase and antiamnestic activities. Planta Med., 65, 218–221 (1999).
Kim, S. R., Kang, S. Y., Lee, K. Y., Kim, S. H., Markelonis, G. J., Oh, T. H., and Kim, Y. C., Anti-amnestic activity of E-p-methoxycinnamic acid from Scrophularia buergeriana. Cog. Brain Res., 17, 454–461 (2003).
Kopelman, M. D. and Corn, T. H., Cholinergic blockade as a model of cholinergic depletion. Brain, 111, 1079–1110 (1988).
Lahiri, D. K., Farlow, M. R., Greig, N. H., and Sambamurti, K., A critical analysis of new molecular targets and strategies for drug developments in Alzheimer’s disease. Drug Develop. Res., 56, 267–281 (2002).
Li, X., Wang, Q., Zhang, L.T., Xu, L., and Yin, W., HPLC study of tissues distribution of loganin in rats. Biomed. Chromatogr. 20, 1087–1092 (2006).
Li, X., Wang, Q., Zhang, L.T., Yin, W., Xu, L., and Li, M., Pharmacokinetic study on loganin from Cornus officinalis Sieb.et Zucc in rats. Chin. J. Pharmaceut. Anal. 27, 4–7 (2007).
Li, X., Huo, C., Wang, Q., Zhang, X., Sheng, X., and Zhang L., Microbial metabolism of loganin by intestinal bacteria and identification of new metabolites in rat. Biomed. Chromatogr., 22, 367–373 (2008).
Ma, X. and Gang, D. R., In vitro production of huperzine A, a promising drug candidate for Alzheimer’s disease. Phytochemistry, 69, 2022–2028 (2008).
Mantle, D., Pickering, A. T., Perry, E. K., Medicinal plant extracts for the treatment of dementia: A review of their pharmacology, efficacy and tolerability. CNS Drugs, 13, 201–213 (2000).
Mathad, V. T., Raj, K., Bhaduri, A. P., Sahai, R., Puri, A., Tripathi, L. M., and Srivastava, V. M., Studies on the profile of immunostimulant activities of modified iridoid glycosides. Bioorg. Med. Chem., 6, 605–611 (1998).
Misane, I. and Ogren, S. O., Selective 5-HT(1A) antagonists WAY 100635 and NAD-299 attenuate the impairment of passive avoidance caused by scopolamine in the rat. Neuropsychopharmacology, 28, 253–264 (2003).
Morris, R. G., Development of a water maze procedure for studying spatial learning in the rat. J. Neurosci. Meth., 11, 47–60 (1984).
Muir, J. L., Page, K. J., Sirinathsinghji, D. J., Robbins, T. W., and Everitt, B. J., Excitotoxic lesions of basal forebrain cholinergic neurons: effects on learning, memory and attention. Behav. Brain Res., 57, 123–131 (1993).
Perry, E. K., Tomlinson, B. E., Blessed, G., Bergman, K., Gibson, P. H., and Perry, R. H., Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia. Br. Med. J., 2, 1457–1459 (1978).
Rubaj, A., Zgodzinski, W., and Sieklucka-Dziuba, M., The influence of adenosine A3 receptor agonist: IB-MECA, on scopolamine- and MK-801-induced memory impairment. Behav. Brain Res., 141, 11–17 (2003).
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk D. C., Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76–85 (1985).
Wang, T. S., Pan, Y., Yin, F., Xu, H. Q., Fang, T. H., and Cai, B. C., Effect of loganin and synephrineon rabbit model with severe hemorrhagic shock. Chin. J. Nanjing Univ. TCM, 15, 345–346 (1999).
Wilcock, G. K., Esiri, M. M., Bowen, D. M., and Smith, C. C. T., Alzheimer’s disease: correlation of cortical choline acetyltransferase activity with the severity of dementia and histological abnormalities. J. Neurol. Sci., 57, 407–417 (1982).
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Lee, K.Y., Sung, S.H., Kim, S.H. et al. Cognitive-enhancing activity of loganin isolated from Cornus officinalis in scopolamine-induced amnesic mice. Arch. Pharm. Res. 32, 677–683 (2009). https://doi.org/10.1007/s12272-009-1505-6
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DOI: https://doi.org/10.1007/s12272-009-1505-6