Abstract
To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was engineered to produce malic acid from glucose by co-localization of two key enzymes phosphoenolpyruvate carboxylase (Ppc) and malate dehydrogenase (MdhA) with synthetic scaffold complex. Scaffold plasmid has produced the maximum concentration of 3.51 g/L malic acid from 10 g/L glucose in 48 h of culture. pH 5.5 and temperature 30°C were optimum for malic acid production without any engineering of competing metabolic pathways. E. coli mutant strains and different concentrations of glucose also tested. When 50 g/L glucose was used as substrate, 20.4 g/L of malic acid was produced.
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Acknowledgements
This work was supported by the 2019 Research Fund of the University of Ulsan.
The authors declare no conflict of interest.
Neither ethical approval nor informed consent was required for this study.
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Somasundaram, S., Jeong, J., Irisappan, G. et al. Enhanced Production of Malic Acid by Co-localization of Phosphoenolpyruvate Carboxylase and Malate Dehydrogenase Using Synthetic Protein Scaffold in Escherichia coli. Biotechnol Bioproc E 25, 39–44 (2020). https://doi.org/10.1007/s12257-019-0269-1
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DOI: https://doi.org/10.1007/s12257-019-0269-1