Abstract
Natural products in Chonnam, the Republic of Korea were screened via anti-angiogenesis experiments, and one candidate product of A. radix extract (ARE) was identified. A. radix extract (ARE) exerted dose-dependent inhibitory effects against angiogenesis, adipogenesis, and cell adhesion. ARE exhibits an angiogenesis inhibitory effect superior to that of the EGCG from the green tea leaves. The expression level of angiogenesis and adipogenesis-related signal molecules in the Western blotting was reduced by increasing the amount of added ARE. Moreover, a diet supplemented with ARE was deemed more effective in inducing weight loss in LB mice than a representative synthetic diet drug, orlistat, which incidentally caused the side effects of denuding the mice of their hair. These results indicate that ARE may prove to be a useful anti-adipogenic compound, and the in vitro results may be reflected at a later time under in vivo conditions.
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Pataky, Z., E. Bobbioni-Harsch, and A. Golay (2009) Obesity: A complex growing challenge. Exp. Clin. Endocr. Diab. 118: 427–433.
Frezza, E. E., M. S. Wachtel, and B. T. Ewing (2006) The impact of morbid obesity on the state economy: an initial evaluation. Surg. Obes. Relat. Dis. 2: 504–508.
Folkman, J. and Y. Shing (1992) Angiogenesis. J. Biol. Chem. 267: 10931–10934.
Doldi, N., M. Bassan, M. Gulisano, V. Broccoli, E. Boncinelli, and A. Ferrari (1996) Vascular endothelial growth factor messenger ribonucleic acid expression in human ovarian and endometrial cancer. Gynecol. Endocrinol. 10: 375–382.
Cao, Y. (2007) Angiogenesis modulates adipogenesis and obesity. J. Clin. Invest. 117: 2362–2368.
Crandall, D. L., G. J. Hausman, and J. G. Kral (1997) A review of the microcirculation of adipose tissue: Anatomic, metabolic, and angiogenic perspectives. Microcircul. 4: 211–232.
Hausman, G. J. and R. L. Richardson (2004) Adipose tissue angiogenesis. J. Anim. Sci. 82: 925–934.
Neels, J. G., T. Thinnes, and D. J. Loskutoff (2004) Angiogenesis in an in vivo model of adipose tissue development. FASEB J. 18: 983–985.
Voros, G., E. Maquoi, D. Demeulemeester, N. Clerx, D. Collen, and H. R. Lijnen (2005) Modulation of angiogenesis during adipose tissue development in murine models of obesity. Endocrinol. 146: 4545–4554.
Christiaens, V. and H. R. Lijnen (2010) Angiogenesis and development of adipose tissue. Mol. Cell. Endocrinol. 318: 2–9.
Rudkowska, I., C. E. Roynette, and I. Demonty, C. A. Vanstone, S. Jew, and P. J. Jones (2005) Diacylglycerol: Efficacy and mechanism of action of an anti-obesity agent. Obes. Res. 13: 1864–1876.
Obeid, O. A., S. T. Bittar, N. Hwalla, and P. W. Emery (2005) Effect of diet supplementation with glutamine, dihydroxyacetone, and leucine on food intake, weight gain, and postprandial glycogen metabolism of rats. Nutrition 21: 224–229.
Tyler, C., A. Prestwich, A. Ormond, and M. MacDougald (2007) Wnt/b-catenin signaling in adipogenesis and metabolism. Curr. Opin. Cell Biol. 19: 612–617.
Yun, J. W. (2010) Possible anti-obesity therapeutics from nature — a review. Phytochem. 71: 1625–1641.
Hwang, J. H. and J. D. Kim (2011) Inhibitory effects of Siegesbeckiae herba extract on angiogenesis and adipogenesis. Biotechnol. Bioproc. Eng. 16: 144–152.
Oh, S., K. S. Kim, Y. S. Chung, M. Shong, and S. B. Park (2009) Anti-obesity agents: A focused review on the structural classification of therapeutic entities. Curr. Top Med. Chem. 9: 466–481.
Huang, L., H. Peng, and P. Xiao (2011) Development trend of traditional Chinese medicine resources. Zhongguo Zhong Yao Za Zhi. 36:1–4.
Ramírez-Zacarías, J. L., F. Castro-Muñozledo, and W. Kuri-Harcuch (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochem. 97: 493–497.
Lee, D. H., J. P. MacIntyre, E. Wang, D. J. Hudson, A. Ishaque, J. A. Conant, B. L. Pope, and C. Y. Lau (1994) A leukocyte lipid up-regulates the avidity of lymphocyte function-associated antigen-1. Biochem. Bioph. Res. Comm. 199: 319–326.
Vandermeeren, M., S. Janssens, M. Borgers, and J. Geysen (1997) Dimethylfumarate is an inhibitor of cytokine-induced Eselectin, VCAM-1, and ICAM-1 expression in human endothelial cells. Biochem. Bioph. Res. Comm. 234: 19–23.
Carmelit, P., M. G. Lampugnani, L. Moons, F. Breviaro, V. Compernolle, F. Bono, G. Balconi, R. Spagnuolo, B. Oosthuyse, M. Dewerchin, A. Zanetti, and A. Angellilo (1999) Targeted deficiency or cystolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell 98: 147–157.
Zhang, D., M. Bar-Eli, S. Meloche, and P. Brodt (2004) Dual regulation of MMP-2 expression by the type 1 insulin-like growth factor receptor: The phosphatidylinositol 3-kinase/Akt and Raf/ERK pathways transmit opposing signals. J. Biol. Chem. 279: 19683–19690.
Ballinger, A. and S. R. Peikin (2002) Orlistat: Its current status as an anti-obesity drug. Eur. J. Pharmacol. 440: 109–117.
Jandacek, R. J. and S. C. Woods (2004) Pharmaceutical approaches to the treatment of obesity. Drug Discov. Today 15: 874–880.
Birari, R. B. and K. K. Bhutani (2007) Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov. Today 12: 879–889.
Weigle, D. S. (2003) Pharmacological therapy of obesity: Past, present, and future. J. Clin. Endocr. Metab. 88: 2462–2469.
Gallo, O., A. Franchi, L. Magnelli, I. Sardi, A. Vannacci, V. Boddi, V. Chiarugi, and E. Masini (2001) Cyclooxygenase-2 pathway correlates with VEGF expression in head and neck cancer. Implications for tumor angiogenesis and metastasis. Neoplasia 3: 53–61.
Prasad, S., J. Ravindran, and B. B. Aggarwal (2010) NF-kappaB and cancer: How intimate is this relationship. Mol. Cell Biochem. 336: 25–37.
Choi, Y. S., H. Park, and S. Jeong (2006) Role of PI3-kinase/akt pathway in the activation of etoposide-induced NF-Kb transcription factor. J. Microbiol. Biotechnol. 16: 391–398.
Tang, F. Y., N. Nhan, and M. Mohsen (2003) Green tea catechins inhibit VEGF-induced angiogenesis in vitro through suppression of VE-cadherin phosphorylation and inactivation of AKT molecule. Int. J. Cancer 106: 871–873.
Oh, I. S. and G. K. Hwan (2004) Vascular endothelial growth factor upregulates follistatin in human umbilical vein endothelial cells. Biotechnol. Bioproc. Eng. 9: 201–206.
Tang, F. Y. and M. Meydani (2001) Green tea catechins and vitamin E inhibit angiogenesis of human microvascular endothelial cells through suppression of IL-8 production. Nutr. Cancer 41: 119–125.
Mistry, T., J. E. Digby, K. M. Desai, and H. S. Randeva (2007) Obesity and prostate cancer: a role for adipokines. Eur. Urol. 52: 46–53.
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Hwang, JH., Lee, SW., Han, KH. et al. Anti-angiogenesis and anti-adipogenesis effects of Anthrisci radix extract. Biotechnol Bioproc E 18, 164–172 (2013). https://doi.org/10.1007/s12257-012-0470-y
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DOI: https://doi.org/10.1007/s12257-012-0470-y