Abstract
Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37°C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Haq, I., H. Ashraf, J. Iqbal, and M. A. Qadeer (2003) Production of alpha amylase by Bacillus licheniformis using an economical medium. Bioresour. Technol. 87: 57–61.
Liming, X. and S. Xueliang (2004) High-yield cellulase production by Trichoderma reesei ZU-02 on corn cob residue. Bioresour. Technol. 91: 259–262.
Olsson, L. and B. Hahn-Hagerdal (1996) Fermentation of lignocellulosic hydrolysates for ethanol production. Enzyme Microb. Technol. 18: 312–331.
Luo, J., L. Xia, J. Lin, and P. Cen (1997) Kinetics of simultaneous saccharification and lactic acid fermentation processes. Biotechnol. Prog. 13: 762–767.
Baek, S. C. and Y. J. Kwon (2007) Optimization of the pretreatment of rice straw hemicellulosic hydrolyzates for microbial production of xylitol. Biotechnol. Bioprocess Eng. 12: 404–409.
Min, B. J., Y. S. Park, S. W. Kang, Y. S. Song, J. H. Lee, C. H. Park, C. W. Kim, and S. W. Kim (2007) Statistical optimization of medium components for the production of xylanase by Aspergillus niger KK2 in submerged cultivation. Biotechnol. Bioprocess Eng. 12: 302–307.
Takagi, M., S. Abe, S. Suzuki, G. H. Emert, and N. Yata (1977) A method for production of ethanol directly from cellulose using cellulase and yeast. pp. 551–571. In: T. K. Ghose. Proceedings of Bioconversion Symposium. Delhi, India.
Ballesteros, M., J. M. Oliva, M. J. Negro, P. Manzanares, and I. Ballesteros (2004) Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875. Process Biochem. 39: 1843–1848.
Saha, B. C. and M. A. Cotta (2007) Enzymatic saccharification and fermentation of alkaline peroxide pretreated rice hull to ethanol. Enzyme Microb. Technol. 41: 528–532.
Saha, B. C., L. B. Iten, M. A. Cotta, and Y. V. Wu (2005) Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol. Biotechnol. Prog. 21: 816–822.
Kim. K. C., S. S. Yoo, Y. A. OH, and S. J. Kim (2003) Isolation and characteristics of Trichoderma harzianum FJ1 producing cellulases and xyanase. J. Microbiol. Biotechnol. 13: 1–8.
Cai, Y. J., S. J. Chapman, J. A. Buswell, and S. T. Chang (1999) Production and distribution of endoglucanase, cellobiohydrolase, and β-glucosidase components of the cellulolytic system of Volvariella volvacea, the edible straw mushroom. Appl. Environ. Microbiol. 65: 553–559.
Brich, P. R., P. F. Sims, and P. Broda (1995) Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chryscsporium. Appl. Environ. Microbiol. 61: 3741–3744.
Chen, H., X. Li, and L. G. Ljungdahl (1994) Isolation and properties of an extracellular β-glucosidase from the polycentric rumen fungus Orpinomyces sp. strain PC-2. Appl. Environ. Microbiol. 60: 64–70.
Kim, K. C., S. W. Kim, M. J. Kim, and S. J. Kim (2005) Saccharification of foodwastes using cellulolytic and amylolytic enzymes from Trichoderma harzianum FJ1 and its kinetics. Biotechnol. Bioprocess Eng. 10: 52–59.
Krishna, C. (1999) Production of bacterial cellulases by solid state bioprocessing of banana wastes. Bioresour. Technol. 69: 231–239.
Wood, T. M. (1992) Fungal cellulases. Biochem. Soc. Trans. 20: 46–53.
Suto, M. and F. Tomita (2001) Induction and catabolite repression mechanisms of cellulase in fungi. J. Biosci. Bioeng. 92: 305–311.
Lee, Y. J., B. K. Kim, B. H. Lee, K. I. Jo, N. K. Lee, C. H. Chung, Y. C. Lee, and J. W. Lee (2008) Purification and characterization of cellulase produced by Bacillus amyloliquefaciens DL-3 utilizing rice hull. Bioresour. Technol. 99: 378–386.
Jung, H. K., J. H. Hong, S. C. Park, B. K. Park, D. H. Nam, and S. D. Kim (2007) Production and physicochemical characterization of β-glucan produced by Paenibacillus polymyxa JB115. Biotechnol. Bioprocess Eng. 12: 713–719.
Lejeune, R. and G. V. Baron (1995) Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation. Appl. Microbiol. Biotechnol. 43: 249–258.
Domingues, F. C., J. A. Queiroz, J. M. S. Cabral, and L. P. Fonseca (2000) The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30. Enzyme Microb. Technol. 26: 394–401.
Lee, S. M. and Y. M. Koo (2001) Pilot-scale production of cellulase using Trichoderma reesei Rut C-30 in fed-batch mode. J. Microbiol. Biotechnol. 11: 229–233.
Okolo, J. C., S. K. C. Obi, and F. J. C. Odibo (1998) Purification and characterization of two distinct carboxymethylcellulases of Paecilomyces sp. Bioresour. Technol. 66: 231–234.
Chen, P., T. Wei, Y. Chang, and L. Lin (2004) Purification and characterization of carboxymethyl cellulase from Sinorhizobium fredii. Bot. Bull. Acad. Sin. 45: 111–118.
Mayende, L., B. S. Wilhelmi, and B. I. Pletschke (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biol. Biochem. 38: 2963–2966.
Ingram, L. O. and J. B. Doran (1995) Conversion of cellulosic materials to ethanol. FEMS Microbiol. Rev. 16: 235–241.
Park, E. Y., Y. Ikeda, and N. Okuda (2002) Empirical evaluation of cellulase on enzymatic hydrolysis of waste office paper. Biotechnol. Bioprocess Eng. 7: 268–274.
Mielenz, J. R. (2001) Ethanol production from biomass: technology and commercialization status. Curr. Opin. Microbiol. 4: 324–329.
Yu, X. B., J. H. Nam, H. S. Yun, and Y. M. Koo (1998) Optimization of cellulase production in batch fermentation by Trichoderma reesei. Biotechnol. Bioprocess Eng. 3: 44–47.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jo, KI., Lee, YJ., Kim, BK. et al. Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3. Biotechnol Bioproc E 13, 182–188 (2008). https://doi.org/10.1007/s12257-007-0149-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12257-007-0149-y