Abstract
The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulation. Rectal application of phage lysates targeted against Enterococcus faecalis cultured from the prostatic fluid gave encouraging results regarding bacterial eradication, abatement of clinical symptoms of prostatitis, and lack of early disease recurrence.
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Abbreviations
- CBP:
-
chronic bacterial prostatitis
- PFU:
-
plaque-forming unit(s)
- DRE:
-
digital rectal examination
- PSA:
-
prostate-specific antigen
- IIET:
-
Institute of Immunology and Experimental Therapy
- PT:
-
phage therapy
- NIH-CPSI:
-
National Institutes of Health Chronic Prostatitis Symptom Index
- Q max :
-
maximum urinary flow rate
- TRUS:
-
transrectal ultrasound (method)
References
Arias C.A., Murray B.E.: Antibiotic-resistant bugs in the 21st century — a clinical super-challenge. New Engl.J.Med. 360, 439–443 (2009).
Benway B.M., Moon T.D.: Bacterial prostatitis. Urol.Clin.N.Am. 35, 23–32 (2008).
Biswas B., Adhya S., Washart P., Paul B., Trostel A.N., Powell B., Carlton R., Merril C.R.: Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium. Infect.Immun. 70, 204–210 (2002).
Drahovská H., Slobodníková L., Kocíncová D., Seman M., Končeková R., Trupl J., Turňa J.: Antibiotic resistance and virulence factors among clinical and food enterococci isolated in Slovakia. Folia Microbiol. 49, 763–768 (2004).
Dworniczek E., Kuzko K., Mróz E., Wojciech Ł., Adamski R., Sobieszczańska B., Seniuk A.: Virulence factors and in vitro adherence of Enterococcus strains to urinary catheters. Folia Microbiol. 48, 671–678 (2003).
Freitag T., Squires R.A., Schmid J.: Naturally occurring bacteriophages lyse a large proportion of canine and feline uropathogenic Escherichia coli isolates in vitro. Res.Vet.Sci. 85, 1–7 (2008).
Górski A., Międzybrodzki R., Borysowski J., Weber-Dąbrowska B., Lobocka M., Fortuna W., Letkiewicz S., Zimecki M., Filby G.: Bacteriophage therapy for the treatment of infections. Curr.Opin.Investig.Drugs 10, 766–774 (2009).
Hoffmann M.: Tierversuche zur Schleimhautpassage und Resorptionsviramie von T3-Phagen nach oraler, trachealer und rektaler Gabe. Z.Bakteriol.Med.-Hyg.Bakteriol.Virusforsch.Parasitol. 198, 371–390 (1965).
Hu W.L., Zhong S.Z., He H.X.: Treatment of chronic bacterial prostatitis with amikacin through anal submucosal injection. Asian J.Androl. 4, 163–167 (2002).
Kurzępa A., Dąbrowska K., Świtała-Jeleń A., Górski A.: Molecular modification of T4 bacteriophage proteins and its potential application. Folia Microbiol. 54, 5–15 (2009).
Lang L.H.: FDA approves use of bacteriophages to be added to meat and poultry products. Gastroenterology 131, 1370 (2006).
Litwin M.S., Mcnaughton-Collins M., Fowler F.J. Jr., Nickel J.C., Calhoun E.A., Pontari M.A., Alexander R.B., Farrar J.T., O’Leary M.P.: The National Institutes of Health Chronic Prostatitis Symptom Index: development and validation of a new outcome measure. Chronic Prostatitis Collaborative Research Network. J.Urol. 162, 369–375 (1999).
Międzybrodzki R., Świtała-Jeleń K., Fortuna W., Weber-Dąbrowska B., Przerwa A., Kurzępa A., Łusiak-Szelachowska M., Dąbrowska K., Boratyński J., Syper D., Ługowski C., Poźniak G., Górski A.: Bacteriophage inhibition of reactive oxygen species generation by endotoxin-stimulated polymorphonuclear leukocytes. Virus Res. 131, 233–242 (2008).
Międzybrodzki R., Fortuna W., Weber-Dąbrowska B., Górski A.: A retrospective analysis of changes in inflammatory markers in patients treated with bacterial viruses. Clin.Exp.Med. (2009); doi: 10.1007/s10238-009-0044-2.
VAN NIEUWKOOP C., VISSER L.G., GROENEVELD J.H., KUIJPER E.J.: Chronic bacterial prostatitis and relapsing Enterococcus faecalis bacteremia successfully treated with moxifloxacin. J.Infect. 56, 155–156 (2008).
Nigutová K., Štyriak I., Javorský P., Pristaš P.: Partial characterization of Enterococcus faecalis bacteriophage F4. Folia Microbiol. 53, 234–236 (2008).
Nishikawa H., Yasuda M., Uchiyama J., Rashel M., Maeda Y., Takemura I., Sugihara S., Ujihara T., Shimizu Y., Shuin T., Matsuzaki S.: T-Even-related bacteriophages as candidates for treatment of Escherichia coli urinary tract infections. Arch. Virol. 153, 507–515 (2008).
OmniLytics: AgriPhage™ product info; http://www.omnilytics.com/products/agriphage/agriphage_info/agriphage_over view.html (accessed 22.5.2009).
Pronk M.J., Pelger R.C., Baranski A.G., van Dam A., Arend S.M.: Cure of chronic prostatitis presumably due to Enterococcus spp. and Gram-negative bacteria. Eur.J.Clin.Microbiol.Infect.Dis. 25, 270–271 (2006).
Schaeffer A.J.: Epidemiology and demographics of prostatitis. Eur.Urol. 2(Suppl.), 5–10 (2003).
Schlegel P.N., Chang T.S., Marshall F.F.: Antibiotics: potential hazards to male fertility. Fertil.Steril. 55, 235–242 (1991).
Shafik A., Mohi-El-Din M.: A new concept of the anatomy of the anal sphincter mechanism and the physiology of defecation — XXIV. Hemorrhoidal venous plexuses: anatomy and role in hemorrhoids. Coloproctology 7, 291–296 (1985).
Ślopek S., Durlakowa I., Weber-Dąbrowska B., Kucharewicz-Krukowska A., Dąbrowski M., Bisikiewicz R.: Results of bacteriophage treatment of suppurative bacterial infections — I. General evaluation of the results. Arch.Immunol.Ther.Exp. 31, 267–291 (1983).
Wagenlehner F.M.E., Diemer T., Naber K.G., Weidner W.: Chronic bacterial prostatitis (NIH type II): diagnosis, therapy and influence on the fertility status. Andrologia 40, 100–104 (2008).
Zhou J.F., Xiao W.Q., Zheng Y.C., Dong J., Zhang S.M.: Increased oxidative stress and oxidative damage associated with chronic bacterial prostatitis. Asian J.Androl. 8, 317–323 (2006).
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Letkiewicz, S., Międzybrodzki, R., Fortuna, W. et al. Eradication of Enterococcus faecalis by phage therapy in chronic bacterial prostatitis — case report . Folia Microbiol 54, 457–461 (2009). https://doi.org/10.1007/s12223-009-0064-z
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DOI: https://doi.org/10.1007/s12223-009-0064-z