Abstract
Bacillus vallismortis strain EXTN-1 is a proven biotic elicitor of systemic resistance in many crops against various pathogens. l-Alanine (Ala) was tested in cucumber as a chemical elicitor of induced systemic resistance (ISR) against Colletotrichum orbiculare. In the greenhouse, both Ala and EXTN-1 induced significant levels of disease suppression in cucumber against anthracnose. When cucumber plants were treated with EXTN-1 and Ala together, augmentative disease suppression was observed. Experiments with transgenic tobacco plants carrying pathogenesis-related genes fused with the β-glucuronidase (GUS) reported gene (PR-1a::GUS & PDF 1.2::GUS) showed an enhanced activation of both PR-1a and PDF 1.2 genes upon combined treatment with Ala and EXTN-1. RT-PCR analysis with transgenic (PR-1a or PDF 1.2 over expressing) Arabidopsis plant showed more enhanced expression of resistance genes PR-1a and PDF 1.2 upon combined treatment with Ala and EXTN-1 than either alone. An augmentative ISR effect, when the bacterial elicitor and chemical elicitor were combined together, was confirmed.
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Abbreviations
- Ala:
-
l-alanine
- BTH:
-
2,1,3-benzothiadiazole
- GUS:
-
β-glucuronidase
- ISR:
-
induced systemic resistance
- Jas:
-
jasmonic acid (2-(cis-2-pentenyl)-3-carboxymethylcyclopentanone)
- RT-PCR:
-
reverse transcriptase polymerase chain reaction
- Sal:
-
salicylic acid (2-hydroxybenzoic acid)
References
Ahn I.P, Park K.S., Kim C.H.: Rhizobacteria-induced resistance perturbs viral disease progress and triggers defense-related gene expression. Mol.Cells13, 302–308 (2002).
Chen J.X., Hall D.E., Murata J., De Luca V.: l-Alanine induces programmed cell death in V. labrusca cell suspension cultures. Plant Sci.171, 734–744 (2006).
Epple P., Apel K., Bohlmann H.: Over-expression of an endogenous thionine enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell9, 509–520 (1997).
Jefferson R.A.: Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol.Biol.Rep.5, 387–405 (1987).
Jeun Y.C., Park K.S., Kim C.H.: Different mechanisms of induced systemic resistance and systemic acquired resistance against Colletotrichum orbiculare on the leaves of cucumber plant. Mycobiology29, 19–26 (2001).
Lawton K.A., Friedrich L., Hunt M., Weyman K., Delaney T., Kessmann H., Staub T., Ryals J.: Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J.10, 71–82 (1996).
Leeman M., VAN Pelt J.A., DEN Ouden F.M., Heinsbroek M., Bakker P.A.H.M., Schippers B.: Induction of systemic resistance by Pseudomonas fluorescens in radish cultivars differing in susceptibility to Fusarium wilt, using a novel bioassay. Eur.J.Plant Pathol.101, 655–664 (1995).
van Loon L.C.: Induced resistance in plants and the role of pathogenesis-related proteins. Eur.J.Plant Pathol.103, 753–765 (1997).
Mitter N., Kazan K., Way H.M, Broekaert W.F., Manners J.M.: Systemic induction of an Arabidopsis plant defensin gene promoter by tobacco mosaic virus and jasmonic acid in transgenic tobacco. Plant Sci.136, 169–180 (1998).
Monselise B.E., Parola A.H., Kost D.: Low-frequency electromagnetic fields induce a stress effect upon higher plants, as evident by the universal stress signal, alanine. Biochem.Biophys.Res.Commun.7, 427–434 (2003).
Park K.S., Kloepper J.W.: Activation of PR-1a promoter by rhizobacteria that induce systemic resistance in tobacco against Pseudomonas syringae pv. tabaci. Biol.Control18, 2–9 (2000).
Park K.S., Ahn I.P., Kim C.H.: Systemic resistance and expression of the pathogenesis-related genes mediated by the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens EXTN-1 against anthracnose disease in cucumber. Mycobiology29, 48–53 (2001).
Park K.S, Paul D., Ye W.H.: Bacillus vallismortis EXTN-1 mediated growth promotion and disease suppression in rice (Oryza sativa L.). Plant Pathol.J.22, 278–282 (2006a).
Park K.S., Paul D., Ryu K.R., Kim E.Y., Kim Y.K.: Bacillus vallismortis strain EXTN-1 mediated systemic resistance against potato virus X and Y (PVX & PVY) in the field. Plant Pathol.J.22, 360–363 (2006b).
von Rad U., Mueller J.M., Durner J.: Evaluation of natural and synthetic stimulants of plant immunity by microarray technology. New Phytol.165, 191–202 (2004).
Reymond P., Framer E.E.: Jasmonate and salicylate as global signals for defense gene expression. Curr.Opin.Plant Biol.1, 404–411 (1998).
Staub T., Ahl G.P., Kessman H.: Chemically induced disease resistance in plants, pp. 239–249 in Proc. 10th Internat. Symp. Systemic Fungicides and Antifungal Compounds (H. Lyr, C. Polter, Eds). Ulmer, Stuttgart (Germany) 1993.
Yoshioka K., Nakashita H., Klessig D.F., Yamaguchi I.: Probenazole induces systemic acquired resistance in Arabidopsis with a novel type of action. Plant J.252, 149–157 (2001).
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Park, K.S., Paul, D., Kim, J.S. et al. l-Alanine augments rhizobacteria-induced systemic resistance in cucumber. Folia Microbiol 54, 322–326 (2009). https://doi.org/10.1007/s12223-009-0041-6
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DOI: https://doi.org/10.1007/s12223-009-0041-6