Abstract
The relatedness between clinical isolates of P. aeruginosa obtained from patients during their stay in a Portuguese Central Hospital was evaluated. Genotypic fingerprinting (M13-PCR), phenotypic methods (biotyping and antibiotyping) and epidemiological information (spatial and temporal links) were used to evaluate the relatedness between 88 clinical isolates (68 patients), selected randomly out of 189. Sixty-two M13 types were found, 12 of them containing isolates from more than one patient. Thirty-four antibiotypes were found, as well as a significant association (p < 0.05) between epidemic isolates and multiresistance patterns. The nosocomial transmission of P. aeruginosa strains may be limited since M13 typing demonstrated a high degree of diversity among all the isolates, suggesting the occurrence of mainly independent infectious episodes. The results show the possible occurrence of cross-acquisition, cross-colonization and cross-infection and suggest an epidemic population structure for P. aeruginosa in this hospital.
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Abbreviations
- ABT(s):
-
antibiotype(s)
- ATCC:
-
American Type Culture Collection
- BS:
-
bronchial secretion
- CA:
-
catheter
- EX:
-
exudate
- HMS:
-
Harvard Medical School
- MDR:
-
multidrug-resistant
- PFGE:
-
pulse field gel electrophoresis
- R:
-
resistant
- REF:
-
reference strain
- S:
-
susceptible
- UR:
-
urine
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Fonseca, A.P., Correia, P., Extremina, C.I. et al. Molecular epidemiology of pseudomonas aeruginosa clinical isolates from Portuguese Central Hospital. Folia Microbiol 53, 540–546 (2008). https://doi.org/10.1007/s12223-008-0086-y
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DOI: https://doi.org/10.1007/s12223-008-0086-y