Abstract
Serratia plymuthica strain IC1270 isolated from the rhizosphere, possessing antagonistic activity towards a wide range of plant-pathogenic fungi, is able to hydrolyze phytate. Phytase activity was found intracellularly, while no activity was detected in the culture liquid. Optimum activity was found at pH 4–5; it completely disappeared at pH > 7.0 and 2.5. Phytase production was practically absent in the exponential phase and reached a maximum in the late stationary phase. Mutations of genes grrA and grrS, encoding GacA/GacS-like 2-component global regulatory system, or in gene rpoS encoding the σ factor RpoS subunit of RNA polymerase, led to a deficiency in phytase production. Introduction into mutants of the respective wild-type genes cloned into the wide-range plasmid pJFF224-NX under the control of the bacteriophage T4 gene 32 promoter complemented this deficiency. This is the first report implicating the GacA/GacS global regulators and RpoS factor in phytase production in bacteria.
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Abbreviations
- Clm:
-
chloramphenicol
- Gen:
-
gentamicin
- Rif:
-
rifampicin
- Tet:
-
tetracycline
- PCR:
-
polymerase chain reaction
- Clmr :
-
chloramphenicol-resistant
- Genr :
-
gentamicin-resistant
- Rifr :
-
rifampicin-resistant
- Tetr :
-
tetracycline-resistant
- PHY(s):
-
phytate(s)
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Shedova, E., Lipasova, V., Velikodvorskaya, G. et al. Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica . Folia Microbiol 53, 110–114 (2008). https://doi.org/10.1007/s12223-008-0016-z
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DOI: https://doi.org/10.1007/s12223-008-0016-z