Abstract
The aim of this study was to compare physical, mechanical and biological properties of 3-dimensional scaffolds prepared from Bombyx mori silk fibroin (SF), fibroin blended with collagen (SF/C), and fibroin blended with gelatin (SF/G) using a freeze-drying technique. The prepared scaffolds were sponge-like structure that exhibited homogeneous porosity with highly interconnected pores. Average pore size of these scaffolds ranged from 65–147 µm. All biodegradable scaffolds were capable of water absorption of 90 %. The degradation behavior of these scaffolds could be controlled by varying the amount of blended polymer. The SF/C and SF/G scaffolds showed higher compressive modulus than that of SF scaffolds which could be attributed to the thicker pore wall observed in the blended constructs. The less crystalline SF structure was observed in SF/G scaffolds as compared to SF/C scaffolds. Thus, the highest compressive modulus was observed on SF/C matrix. To investigate the feasibility of the scaffolds for cartilage tissue engineering application, rat articular chondrocytes were seeded onto the scaffolds. The MTT assay demonstrated that blending collagen or gelatin into SF sponge facilitated cell attachment and proliferation better than SF scaffolds. The blended SF scaffolds possessed superior physical, mechanical and biological properties in comparison to SF scaffolds and showed high potential for application in cartilage tissue engineering.
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References
S. Marlovits, P. Zeller, P. Singer, C. Resinger, and V. Vécsei, Eur. J. Radiol., 57, 24 (2006).
J. Martel-Pelletier, C. Boileau, J.-P. Pelletier, and P. J. Roughley, Best Practice and Research Clinical Rheumatology, 22, 351 (2008).
C. Chung and J. A. Burdick, Adv. Drug Delivery Rev., 60, 243 (2008).
S. R. Frenkel and P. E. Di Cesare, Ann. Biomed. Eng., 32, 26 (2004).
A. Getgood, T. P. S. Bhullar, and N. Rushton, Orthopaedics and Trauma, 23, 189 (2009).
M. V. Risbud and M. Sittinger, Trends in Biotechnology, 20, 351 (2002).
M. Sato, M. Ishihara, K. Furukawa, N. Kaneshiro, T. Nagai, G. Mitani, T. Kutsuna, N. Ohta, M. Kokubo, T. Kikuchi, H. Sakai, T. Ushida, M. Kikuchi, and J. Mochida, Medical & Biological Engineering & Computing, 46, 735 (2008).
R. Stoop, Injury, 39, 77 (2008).
K. F. Leong, C. M. Cheah, and C. K. Chua, Biomaterials, 24, 2363 (2003).
C. Liu, Z. Xia, and J. T. Czernuszka, Chem. Eng. Res. Des., 85, 1051 (2007).
H.-Y. Cheung, K.-T. Lau, T.-P. Lu, and D. Hui, Compos. Part B: Eng., 38, 291 (2007).
J. M. Dang and K. W. Leong, Adv. Drug Delivery Rev., 58, 487 (2006).
D. Grande, C. Halberstadt, G. Naughton, R. Schwartz, and R. Manji, J. Biomed Mater. Res., 34, 211 (1997).
J. G. Hardy, L. M. Römer, and T. R. Scheibel, Polymer, 49, 4309 (2008).
J. G. Hardy and T. R. Scheibel, Prog. Polym. Sci., 35, 1093 (2010).
U. Hersel, C. Dahmen, and H. Kessler, Biomaterials, 24, 4385 (2003).
S. Janjanin, W.-J. Li, M. T. Morgan, R. M. Shanti, and R. S. Tuan, J. Surg. Res., 149, 47 (2008).
K. Lee, D. Kaplan, and J. Velema, “Biopolymer-Based Biomaterials as Scaffolds for Tissue Engineering”, Vol.102, Springer Berlin / Heidelberg, pp.187–238, 2006.
S.-T. Li, “Biomaterials: Principles and Applications” (J. B. Park and J. D. Bronzino Eds.), Vol.1, CRC Press LLC, 2003.
P. B. Malafaya, G. A. Silva, and R. L. Reis, Adv. Drug Delivery Rev., 59, 207 (2007).
B. B. Mandal and S. C. Kundu, Macromolecular Bioscience, 8, 807 (2008).
M. Kawanishi, T. Ushida, T. Kaneko, H. Niwa, T. Fukubayashi, K. Nakamura, H. Oda, S. Tanaka, and T. Tateishi, Mater. Sci. Eng.: C, 24, 431 (2004).
C. D. Hoemann, J. Sun, A. Légaré, M. D. McKee, and M. D. Buschmann, Osteoarthritis and Cartilage, 13, 318 (2005).
R. Nazarov, H.-J. Jin, and D. L. Kaplan, Biomacromolecules, 5, 718 (2004).
Q. Lu, Q. Feng, K. Hu, and F. Cui, J. Mater. Sci.: Mater. in Med., 19, 629 (2008).
Q. Lv, Q. Feng, K. Hu, and F. Cui, Polymer, 46, 12662 (2005).
W. Friess, European Journal of Pharmaceutics and Biopharmaceutics, 45, 113 (1998).
T. Ohno, K. Tanisaka, Y. Hiraoka, T. Ushida, T. Tamaki, and T. Tateishi, Mater. Sci. Eng. C, 24, 407 (2004).
M. D. Shoulders and R. T. Raines, Annu. Rev. Biochem., 78, 929 (2009).
S. Young, M. Wong, Y. Tabata, and A. G. Mikos, Journal of Controlled Release, 109, 256 (2005).
A. Facchini, G. Lisignoli, S. Cristino, L. Roseti, L. De Franceschi, E. Marconi, and B. Grigolo, Biorheology, 43, 471 (2006).
H. Fan, H. Liu, S. L. Toh, and J. C. H. Goh, Biomaterials, 29, 1017 (2008).
M. Garcia-Fuentes, A. J. Meinel, M. Hilbe, L. Meinel, and H. P. Merkle, Biomaterials, 30, 5068 (2009).
Q. Lu, K. Hu, Q. Feng, and F. Cui, Mater. Sci. Eng. C, 29, 2239 (2009).
Q. Lv, K. Hu, Q. Feng, F. Cui, and C. Cao, Compos. Sci. Technol., 67, 3023 (2007).
R. Rohanizadeh, M. Swain, and R. Mason, J. Mater. Sci.: Mater. Med., 19, 1173 (2008).
C.-H. Chang, H.-C. Liu, C.-C. Lin, C.-H. Chou, and F.-H. Lin, Biomaterials, 24, 4853 (2003).
P. Giannoni and R. Cancedda, Cells Tissues Organs, 184, 1 (2006).
D. J. Griffon, M. R. Sedighi, D. V. Schaeffer, J. A. Eurell, and A. L. Johnson, Acta Biomaterialia, 2, 313 (2006).
J. Iwasa, L. Engebretsen, Y. Shima, and M. Ochi, Knee Surgery, Sports Traumatology, Arthroscopy, 17, 561 (2009).
G. T. Kose, F. Korkusuz, A. Özkul, Y. Soysal, T. Özdemir, C. Yildiz, and V. Hasirci, Biomaterials, 26, 5187 (2005).
Y. Stark, K. Suck, C. Kasper, M. Wieland, M. van Griensven, and T. Scheper, Experimental and Toxicologic Pathology, 57, 305 (2006).
R. S. Tigli and M. Gumusderelioglu, International Journal of Biological Macromolecules, 43, 121 (2008).
Y. Wang, D. J. Blasioli, H.-J. Kim, H. S. Kim, and D. L. Kaplan, Biomaterials, 27, 4434 (2006).
H. Wu, Y. Wan, X. Cao, and Q. Wu, Acta Biomaterialia, 4, 76 (2008).
J. Gu, X. Yang, and H. Zhu, Mater. Sci. Eng. C, 20, 199 (2002).
I. Um and Y. Park, Fiber. Polym., 8, 579 (2007).
B. B. Mandal, J. K. Mann, and S. C. Kundu, Eur. J. Pharm. Sci., 37, 160 (2009).
U.-J. Kim, J. Park, H. J. Kim, M. Wada, and D. L. Kaplan, Biomaterials, 26, 2775 (2005).
N. Mohan, P. Nair, and Y. Tabata, J. Mater. Sci.: Mater. Med., 20, 49 (2009).
Q. Lv and Q. Feng, J. Mater. Sci.: Mater. Med., 17, 1349 (2006).
J. Zhan, X. Sun, F. Cui, and X. Kong, Chinese Science Bulletin, 52, 1791 (2007).
T. Hino, M. Tanimoto, and S. Shimabayashi, J. Colloid and Interf. Sci., 266, 68 (2003).
F. Lin, Y. Li, J. Jin, Y. Cai, K. Wei, and J. Yao, Mater. Chem. Phys., 111, 92 (2008).
E. Sashina, A. Bochek, N. Novoselov, and D. Kirichenko, Russian J. Appl. Chem., 79, 869 (2006).
D. Wilson, R. Valluzzi, and D. Kaplan, Biophysical J., 78, 2690 (2000).
L. D. Harris, B.-S. Kim, and D. J. Mooney, J. Biomedical Mater. Res., 42, 396 (1998).
J. Ratanavaraporn, S. Damrongsakkul, N. Sanchavanakit, T. Banaprasert, and S. Kanokpanont, J. Metals, Mater. Minerals, 16, 31 (2006).
Y. Wang, D. D. Rudym, A. Walsh, L. Abrahamsen, H.-J. Kim, H. S. Kim, C. Kirker-Head, and D. L. Kaplan, Biomaterials, 29, 3415 (2008).
C. H. Lee, A. Singla, and Y. Lee, Int. J. Pharmaceutics, 221, 1 (2001).
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Tiyaboonchai, W., Chomchalao, P., Pongcharoen, S. et al. Preparation and characterization of blended Bombyx mori silk fibroin scaffolds. Fibers Polym 12, 324–333 (2011). https://doi.org/10.1007/s12221-011-0324-9
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DOI: https://doi.org/10.1007/s12221-011-0324-9