Abstract
Objective
To explore the imaging features of adrenal primitive neuroectodermal tumors (PNETs).
Materials and methods
This retrospective study included seven patients with surgically and pathologically confirmed adrenal PNETs. Among them, six underwent computed tomography (CT) scans, and one underwent magnetic resonance imaging. The imaging findings, including size, shape, margin, hemorrhage, calcification, cystic degeneration, regional lymph nodes involvement, tumor thrombus formation and enhancement pattern, were retrospectively analyzed.
Results
Among the seven adrenal PNET patients, six were male, and one was female. The median age was 26 years (range 2–56 years). The disease generally presented with either insidious symptoms (n = 4) or non-specific symptoms, including right flank pain (n = 1) and left upper abdominal discomfort (n = 2). On the pre-enhanced CT images, the tumor usually appeared as a well-defined, rounded or oval, heterogeneous mass without calcification. Certain tissue characteristics, such as cystic degeneration (n = 5), capsule (n = 4) and hemorrhage (n = 2), were observed. Regional lymph node involvement was observed in three cases, and renal vein thrombus was observed in one case. All cases showed mild heterogeneous enhancement of the tumor on the enhanced CT images.
Conclusion
An adrenal PNET commonly presents as a relatively large, well-defined, heterogeneous mass with cystic degeneration, necrosis and a characteristic mild contrast-enhancement pattern on multiphase enhanced images. PNET should be considered when the diagnosis of common tumors is not favored by signs on images.
Clinical Trial Registration Statement
This study was approved by the medical ethics committee of Xiangya Hospital, Central South University. The approval number is 201512538.
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Introduction
Primary neuroectodermal tumors (PNETs), which are part of the Ewing’s sarcoma (EWS) family of tumors, are highly malignant tumors comprising small round cells with a neuroectodermal origin and occur primarily in children and adolescents. PNETs are divided into two types based on the location in the body: central primary neuroectodermal tumors [1] and peripheral primitive neuroectodermal tumors (pPNETs) [2]. pPNETs have a predilection for the chest wall (Askin’s tumor), trunk and lower extremities [3,4,5]. PNETs have rarely been reported in the head and neck, spinal column or retroperitoneum (kidney, pancreas and uterus) [5,6,7,8]. PNETs presenting in the adrenal gland show a correspondence in age with adrenocortical carcinoma and malignant pheochromocytoma, and all these tumors are extremely rare.
Approximately 21 cases of adrenal PNETs have been reported in the literature, and most of those studies are case reports. The imaging characteristics of adrenal PNETs are largely unknown [9,10,11,12,13]. It is easy to misdiagnose PNET as another disease arising from the adrenal glands or the retroperitoneal organs during clinical practice. In addition, a timely and accurate preoperative diagnosis of an adrenal tumor as a PNET may be particularly important because of its high rate of malignancy and early metastasis [14]. Here, we conducted a retrospective study to describe the imaging features of this disease to improve preoperative diagnosis of PNET.
Materials and methods
Design and patients
This retrospective study was approved by the hospital’s Institutional Review Board. Written or verbal informed consent was obtained from all patients. From January 2005 to April 2016, seven patients at our hospital were surgically and pathologically confirmed to have primary adrenal PNETs. Based on their financial situations, six underwent complete computed tomography (CT) scans, and one underwent a magnetic resonance imaging (MRI) examination. The clinical and radiological data were collected.
CT and MR imaging
The CT examination was performed with a 320-detector volume CT system (Aquilion ONE, Toshiba, Japan) or a Somatom Definition dual-source CT (DSCT, Siemens Medical System, Forchheim, Germany). The scan parameters were as follows: 5-mm section thickness, 1-mm slice thickness reconstructions, 120-kV voltage, 90- to 270-mA current, and 256 × 256 matrix. A 100-mL intravenous bolus dose of a non-ionic iodinated contrast agent (iopromide; Ultravist; Schering AG, Berlin, Germany) was administered at a rate of 3–4 mL/s. MR images were obtained with a 1.5-T superconductive unit (Singa HD xt, GE Medical Systems, United States), and an abdominal coil was used. The sequences included coronal turbo spin echo T2-weighted images and transverse T1-/T2- and diffusion-weighted images. The parameters of these sequences were repetition time (TR)/echo time (TE) = 4/1.8 ms for coronal T2-weighted images; TR/TE = 165/1.3 ms for transverse T1-weighted images; TR/TE = 9230/92 ms for transverse T2-weighted images; TR/TE = 5000/69 ms for diffusion-weighted images; fields of view of 65 × 65 for transverse images and 100 × 100 for coronal images; a matrix of 512 × 512; 4–6 signal acquisition; and a slice thickness/gap of 3/0.3 mm. Contrast-enhanced coronal and transverse T1-weighted images were obtained after an intravenous injection of gadopentetate dimeglumine (Magnevist, Schering, Berlin, Germany) at a dose of 0.2 mmol/kg at a rate of 1.5 mL/s.
Imaging analysis
Three radiologists specializing in abdominal diseases independently evaluated CT and MRI features of the imaging data, including size, shape, margin, hemorrhage, calcification, attenuation/signal, regional lymph nodes, thrombus, and enhancement features after the intravenous contrast medium injection, and disagreements were resolved by consensus. Areas of suspected hemorrhage were analyzed when the CT attenuation values fell in the range of 50–90 Hu on unenhanced scans [15, 16] or in cases of short T1- and long T2-signal changes on the MR images. Data analysis of CT images was performed based on regions of interest (ROIs). Each ROI was drawn manually on the solid parts three times, and the average CT attenuation was then calculated.
Results
Clinical data
The six men and one woman in this study had a median age of 26 years (range 2–56 years). The clinical symptoms that contributed to the diagnosis were diverse and non-specific, and included left upper abdominal discomfort (n = 2, 28.6%); right flank pain (n = 1, 14.3%); and incidental findings (n = 4, 57.1%). The duration of symptoms varied from 2 days to 1 month. Laboratory results showed no evidence of hormonal hypersecretion (vanillylmandelic acid, 17-hydroxycorticosteroids, 17-ketosteroids) in four patients, one of whom had a high level of neuron-specific enolase (NSE) (greater than 370 ng/mL).
Imaging findings
The imaging findings of the seven adrenal PNET cases are shown in Table 1. Each case exhibited a single tumor for a total of seven tumors. Of these, six (6/7, 85.7%) were located on the left adrenal gland, and the remaining one (1/7, 14.3%) was on the right side. All tumors were well defined. The mean long diameter of the tumors was 7.24 cm. The tumors were round in five (5/7, 71.4%) cases and oval in two (2/7, 28.6%) cases. Among the seven patients, cystic degeneration was observed in five (5/7, 71.4%) (Figs. 1, 2, 3, 4, 5, 6). Capsule was observed in four (4/7, 57.1%) cases, and hemorrhage was observed in two (2/7, 28.6%) cases (Figs. 2, 6). No calcifications were observed. Regional lymph node involvement was observed in three (3/7, 42.9%) cases (Fig. 1, 6), and renal vein thrombus was observed in one (1/7, 14.3%) case (Fig. 2).
The tumors showed mild heterogeneous enhancement patterns on the contrast-enhanced CT images (Figs. 1, 2, 3, 4, 5, 6), with mean CT attenuations of 47.9 Hu (range 43.6–52.8 Hu) on the pre-enhanced CT images, 56.8 Hu (range 52.2–66.1 Hu) in the arterial phase, and 64.5 Hu (range 53.2–82.9 Hu) in the venous phase. Thin rim enhancement and neovascularization of the masses were observed in four (4/7, 57.1%) patients (Fig. 1, 3, 5, 6) and one patient (Fig. 1), respectively.
Surgical and pathological findings
All patients underwent an adrenalectomy, and all tumors originated from the adrenal glands. The tumors appeared as soft, well-circumscribed oval or rounded masses with a tan-yellow appearance. All tumors were heterogeneous in texture, particularly the larger tumors. Cystic degeneration, necrosis (n = 5) and hemorrhage (n = 2) were observed.
Hematoxylin and eosin staining showed that the adrenal medulla was completely replaced by a malignant tumor comprising small, round cells arranged in cords or rosettes (Figs. 2, 3) with a compressed rim of the adrenal cortex at the periphery. Immunohistochemical staining (Table 2) revealed that six (6/7, 85.7%) tumors were positive for CD99; six (6/7, 85.7%) were positive for vimentin; four (4/7, 57.1%) were positive for NSE, five (5/7, 71.4%) were positive for synaptophysin (Syn), and five (5/7, 71.4%) were positive for S-100 protein. The Ki67 index in the seven cases varied from approximately 30 to 90%, indicating that the tumors had high proliferation activity.
Discussion
PNET and EWS are considered to be two stages of the same tumoral entity, and they share a unique and specific t(11;22)(q24;q12) chromosomal translocation, although they can be distinguished by the presence or absence of neural differentiation. The immunohistochemical markers of neuroepithelial differentiation, such as CD99, vimentin, NSE, Syn, and S-100 protein, are frequently positive in PNETs but not in EWS [17]. Pathological diagnosis of PNET depends on the presence of rosettes and pseudorosettes consisting of small and round cells, and two or more positive neural markers.
Primary adrenal PNETs are extremely rare. The adrenal medulla originates from embryonic neural crest. From this perspective, PNET may have histological origin in the adrenal gland. Similar to other reports [9,10,11,12,13], the adrenal PNET patients in our study all tended to present with insidious or non-specific symptoms, such as abdominal or flank pain, that were likely secondary to the compression effect of the tumor.
In this study, all seven masses were relatively large, with a mean diameter of 7.6 cm. This may be due to the fact that the retroperitoneal space surrounding the adrenal glands is relatively large, and tumors growing in the adrenal region are less palpable through the body surface. Therefore, they are likely to be discovered only after they have become enlarged. This also explains why indolent enlargement of the mass is presumed to be primary clinical manifestation. In addition, symptoms generally do not appear until the displacement of the kidney or other adjacent organs becomes overt.
The tumors in this study were solitary rounded and oval masses with well-defined margins and a complete capsule on CT/MR images, consistent with previous reports that adrenal PNETs usually appear as unilateral, rounded or oval solitary masses with well-defined margins [12, 13]. In addition, a well-defined capsule and thin rim enhancement were observed in 57.1% (4/7) of patients. This may suggest an expansive rather than an infiltrative growth pattern because most benign tumors are well-defined encapsulated masses [18]. However, a complete capsule does not absolutely indicate an early-stage tumor with benign biological behavior. In malignant tumors, rapid growth of the tumor cells leads to a rapid increase in tumor volume and suppression of the surrounding connective and fibrous tissues, which in turn results in the formation of a “false capsule” and a well-defined tumor margin. Therefore, this context may represent a rapid growth pattern of tumor cells, but it does not mean that a tumor is non-invasive. The adrenal PNET may be such an example.
Calcification is uncommon in PNETs [18, 19]. Li et al. reported calcification in only 1 of 12 PNETs in the abdominopelvic region [18], while Xu et al. reported that none of the PNETs among 14 patients was positive for calcification [17]. In our study, we did not observe calcification in any of the seven tumors by CT/MRI scans. In addition, hemorrhage was found in 28.6% (2/7) of the patients in this study. Among those, one presented with patchy, high-density areas with an average CT attenuation value of 56.3 Hu, and the other showed hyperintense signals on both T1- and T2-weighted images.
Consistent with previous studies [20, 21], the adrenal PNET cases in this study appeared as heterogeneous, soft-tissue density/signal masses with patchy fluid attenuation areas and mild heterogeneous enhancement. Notably, degenerative changes, such as cystic degeneration and necrosis, were secondary due to an inadequate blood supply to the corresponding region in the tumor. Microscopically, the tumor comprised sparsely scattered stromal elements and a large number of compactly arranged, small, blue, round cells, which appeared to be uniform and had ovoid hyperchromatic irregular nuclei and scant cytoplasms [13]. Because dense tumor cells occupy a significant amount of space, the proportion of stromal elements such as blood vessels is relatively small. This may partially explain the relatively weak enhancement of tumors on the enhanced images.
Because of the high malignancy and invasiveness of PNETs, they often demonstrate regional lymph node involvement, distant metastases and local recurrence after treatment [6, 17, 18]. In our study, regional lymph node involvement was observed in 42.9% (3/7) of patients, and renal vein tumor embolus and local recurrence were found in one patient, respectively. To avoid unnecessary injury during surgery, it is very important to recognize the vessels supplying the adrenal tumors prior to surgery. CT angiography (CTA) techniques are very effective at displaying tumor-feeding vessels. In our study, one patient underwent a CTA examination. The tumor-feeding artery originated from the coeliac trunk, and neovascularization within the tumor was clearly presented on the CTA images.
The differential diagnosis of an adrenal PNET involves a variety of tumors that primarily include nonfunctioning benign or malignant tumors. As the most common type of adrenal tumor, a lipid-rich adenoma is easy to exclude based on its characteristic attenuation value of less than 10 Hu on pre-enhanced CT images [16]. Lipid-poor adenomas can be differentiated from non-adenomas by the characteristic rapid washout of contrast materials in delayed-phase images [16, 22]. Adrenal ganglioneuromas always present as homogenous, well-defined oval, crescentic or lobulated masses with slight to moderate enhancement. An adrenal schwannoma usually appears as a well-defined rounded or oval solid or cystic mass with septation and progressive contrast enhancement [23]. An adrenal abscess should be considered when a patient presents with fever and immune system disorders, rapid growth, and colliquation transformation [23].
Malignant adrenal tumors, such as adrenocortical carcinoma, pheochromocytoma and adrenal metastasis, should also be differentiated from adrenal PNETs. Adrenocortical carcinoma is rare and is characterized by a large (>6 cm in diameter) infiltrative growth, local invasion, and distant metastasis [24]. Unlike an adrenal PNET, a malignant pheochromocytoma is characterized by heterogeneously marked enhancement. Adrenal metastases are common in the unilateral or bilateral adrenal glands. A history of malignancy plays an important role in the diagnosis of adrenal metastases, which typically present as homogeneous, round, soft-tissue masses when the tumor is relatively small and heterogeneous masses with a thick enhancing rim in larger tumors [10, 23, 24].
Conclusion
In summary, primary adrenal PNETs are extremely rare and difficult to diagnose preoperatively. Some imaging features may be suggestive of the diagnosis of an adrenal PNET. Generally, when a well-defined, unilateral, rounded or oval, relatively large soft-density adrenal mass with mild heterogeneous enhancement is encountered incidentally, and the diagnosis of a common tumor is not supported by radiological signs on CT/MR images, the possibility of a PNET should be considered preoperatively.
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Acknowledgements
This study was supported by the Freedom Exploration Program of Central South University (No. 2011QNZT153) and Natural Science Foundation of Hunan Province (No. 14JJ6001).
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This study was conducted in accordance with the Declaration of Helsinki, all its amendments, and national regulations. This retrospective study was approved by the hospital’s Institutional Review Board. Written or verbal informed consent was obtained from all patients.
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None of the authors has declared any conflict of interest.
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Y. Zhang, P. Cai and M. Chen contributed equally to this work.
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Zhang, Y., Cai, P., Chen, M. et al. Imaging findings of adrenal primitive neuroectodermal tumors: a series of seven cases. Clin Transl Oncol 19, 641–649 (2017). https://doi.org/10.1007/s12094-016-1580-3
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DOI: https://doi.org/10.1007/s12094-016-1580-3