Abstract
Some pseudomands are being utilized as biofertilizers and biopesticides because of their role in plant growth promotion and plant protection against root parasites, respectively. Two strains of Pseudomonas, P. jessenii LHRE62 and P. synxantha HHRE81, recovered from wheat rhizosphere, have shown their potential in field bioinoculation tests under rice-wheat and pulse-wheat rotation systems. Normally, pseudomonads are cultivated on synthetic media-like King’s B and used for inoculation on seeds/soil drench with talcum or charcoal as carrier material. Cow dung is being used for different purposes from the ancient time and has a significant role in crop growth because of the content in humic compounds and fertilizing bioelements available in it. Here, cow dung extract was tested as a growth medium for strains LHRE62 and HHRE81, in comparison with growth in King’s B medium. The log phase was delayed by 2 h as compared to growth in King’s B medium. The bacterial growth yield, lower in plain cow dung extract as compared to King’s B medium, was improved upon addition of different carbon substrates. Growth of rice var. Pant Dhan 4 in pot cultures was increased using liquid formulation of cow dung extract and bacteria as foliar spray, compared to their respective controls. Biocontrol efficacy of the bioagents was assessed by challenging rice crop with Rhizoctonia solani, a sheath blight pathogen. The growth promotion and biocontrol efficiencies were more pronounced in the case of mixed inocula of strains LHRE62 and HHRE81.
Article PDF
Avoid common mistakes on your manuscript.
References
Haas D and Défago G (2005) Biological control of soilborne pathogens by fluorescent pseudomonads. Nature Rev Microbiol 3:307–319
Kloepper JW, Lifshitz R and Zablotowicz M (1989) Free living bacterial inocula for enhancing crop productivity. Trends Biotechnol 7:39–44
Srivastava R, Roesti D and Sharma AK (2007) Evaluation of microbial diversity in vegetable based cropping system under organic farming. Appl Soil Ecol 36:116–123
Patino-Vera M, Jimenez B, Balderas K, Ortiz M, Allende R, Carrillo A and Galindo E (2005) Pilot-scale production and liquid formulation of Rhodotorula minuta, a potential biocontrol agent of mango anthracnose. J Appl Microbiol 99:540–550
Dhama K, Rathore R, Chauhan RS and Tomar S (2005) Panchgavya (Cowpathy): An overview. Int J Cow Sci 1: 1–15
Chauhan RS (2000) Panchgavya dwara prakritik chikitsa. Kheti Sansar 3:21–23
Gaur R, Shani N, Kawaljeet, Johri BN, Rossi P and Aragno M (2004) Diacetylphloroglucinol-producing pseudomonads do not influence AM fungi in wheat rhizosphere. Curr Sci 86:453–457
Roesti D (2005) Bacterial community associated to the rhizosphere of wheat: interactions with arbuscular mycorrhizal fungi and selection of plant growth promoting rhizobacteria for the increase of wheat growth and soil health in Indian marginal fields. Ph.D. thesis, University of Neuchatel, Switzerland, pp 1–179
Roesti D, Gaur R, Johri BN, Imfeld G, Sharma AK, Kawaljeet and Aragno M (2006) Plant growth stage, fertilizer management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biol Biochem 38:1111–1120
Nautiyal CS, Mehta S, Singh HB and Pushpangadan P (2006) Go mutra and dung based products as viable alternative for sustainable agriculture for our rural marginal farmers. The Ind Cow 2(9):13–21
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Srivastava, R., Aragno, M. & Sharma, A.K. Cow dung extract: a medium for the growth of pseudomonads enhancing their efficiency as biofertilizer and biocontrol agent in rice. Indian J Microbiol 50, 349–354 (2010). https://doi.org/10.1007/s12088-010-0032-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-010-0032-y