Abstract
We reported the optimum amount of PHB accumulated by Alcaligenes faecalis during its 24 h growth under nitrogen deficient conditions. After 24 h incubation decrease in the amount of PHB was recorded. Hypochlorite digestion of biomass of organism followed by extraction with a solvent system consisting of 1:1 mixture of ethanol and acetone resulted in efficient recovery of PHB vis-à-vis earlier methods. This solvent system gave a high recovery yield, i.e. 5.6 gL−1 vis-à-vis earlier reported yield, 1.34 gL−1 (by same method), 0.63 gL−1 (by chloroform extraction method) and 1.1 gL−1 (by dispersion method).
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anderson AJ and Dawes EA (1990) Occurrence metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkonates. Microbiol Rev 54:450–472
Berger E, Ramsay BA, Ramsay JA and Chavaric C (1989) PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol Tech 31(4):227–230
Holmes P A (1988) Biologically produced PHA polymers and copolymers In: Bestle DC (ed) Developments in crystalline polymers Vol. 2, Elsevier, London, England, pp. 1–65
Rawte T and Mavinkurve S (2001) Biodegradable plastics-bacterial polyhydroxyalkonates. Indian J Microbiol 41:233–245
Young-Mi Jumg, Park Jin-Seo, Young-Hyun Lee (2000) Metabolic engineering of Alcaligenes eutrophus through the cloned phbCAB genes for the investigation of the regulatory mechanism of polyhydroxyalkonate biosynthesis. Enz Microb Technol 26:201–208
Saito Y, Nakamura S, Hiramitsu M and Doi Y. (1996) Microbial synthesis and properties of poly (3-hydroxybutyrate-co-4-hydroxybutyrate). Pol Interntl 39:169–172
Jaimes C, Dobreva-Schue R, Giani-Beaune O, Schue F, Amass W and Amass A (1999) Enzymatic degradability of PHB sterioisomers and coploymers of â-butyrolactone with å- caprolactone and ä valerolactone. Pol Interntl 48:23–32
Choi J, Lee SY (1997) Process analysis and economic evaluation for poly (3-hydroxybutyrate) production by fermentation. Biopro Eng 17:335–338
Lee SY (1996) Bacterial polyhydroxy-alkonates. Biotechnol Bioeng 49:1–14
Reusch RN, Hiske TW, Sadoff HL, Harris R and Beveridge T (1987) Cellular incorporation of poly-â-hydroxbutyrate into plasma membranes of Escherichia coli and Azotobacter vinelandii, alters native membrane structure. Can J Microbiol 33:435–444
Steinbuchel A (1991) Polyhydroxyalkanoic acid. In: Biomaterials: Novel materials from biological source (Byrom D eds), Stockton Press, New York, pp, 124–213
Doi Y, Kawaguchi Y, Nakamura Y and Konioka M (1989) Nuclear Magnetic Resonance studies of poly (3-hydroxybutyrate) and phosphate metabolism in A. eutrophus. Appl Env Microbiol 55:2932
Yellore V and Desai A (1998) Production of native poly-â-hydroxybutyrate from lactose and whey by Methylobacterium sp. ZP24. Lett Appl Microbiol 26:391–394
Hann SK, Chang YK, Kim BS, Lee KM and Chang HN (1993) The recovery of poly (3-hydroxybutyrate) by using dispersion of sodium chloride solution and chloroform. Biotechnol Bioeng 7:209–212
Hann SK, Chang YK, Kim BS, Lee KM and Chang HN (1994) Optimization of microbial (3-hydroxybutyrate) recovery using dispersion of sodium hypochlorite solution and chloroform. Biotechnol Bioeng 44:256–259
Rawte T and Mavinkurve S (2002) A rapid hypochlorite method for the extraction of polyhydroxy alkonates from bacterial cells. Indian J Exptl Biol 40:924–929
Kim BS, Lee SC, Lee SY, Chang HN, Chang YK and Woo SI (1994) Production of poly (3-hydroxy butyric acid co-3-hyroxyvaleric acid) by fed batch culture of Alcaligenes eutrophus with substrate feeding using online glucose analyzer. Biotechnol Bioeng 43:892–898
Rapske R and Rapske AC (1976) Quantitative requirements for exponential growth of Alcaligenes eutrophus. Appl Env Microbiol 32:585–591
Law JH and Slepecky RA (1961) Assay of poly-â-hydroxbutyric acid. J Bacteriol 82:33–36
Smibert RN and Krieg NR (1981) General characterization. In: Manual of methods of general bacteriology (Gerhardt RG, Murray E, Costilow RN, Nester EW, Wood WA, Krieg NR and Phillips GB eds), American Society for Microbiology, Washington, pp. 409–443
Sayyed RZ and Chincholkar SB (2004) Production of Poly - â-hydroxy butyrate (PHB) from Alcaligenes faecalis. Indian J Microbiol 44 (4):269–272
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sayyed, R.Z., Gangurde, N.S. & Chincholkar, S.B. Hypochlorite digestion method for efficient recovery of PHB from Alcaligenes faecalis . Indian J Microbiol 49, 230–232 (2009). https://doi.org/10.1007/s12088-009-0036-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-009-0036-7