Abstract
Mango ginger (Curcuma amada Roxb.) rhizome is used in the manufacture of pickles and other food preparations due to its unique raw mango flavour. The chloroform extract of mango ginger rhizome was subjected to antibacterial activity-guided purification by repeated silica gel column chromatography to obtain a pure compound. The structure of the isolated compound was deduced by analysing UV, IR, LC-MS and 2D-HMQCT NMR spectral data, and named it as amadaldehyde, a novel compound. It exhibited a wide range of antibacterial activity with potential bactericidal activity against several bacteria. The purified compound also exhibited antioxidant activity, cytotoxicity and platelet aggregation inhibitory activities.
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Abbreviations
- ADP:
-
adenosine diphosphate
- BHA:
-
butylated hydroxy anisole
- DMEM:
-
Dulbecco modified Eagle medium
- DMEMPR:
-
Dulbecco modified Eagle medium without phenol red
- DMRT:
-
Duncan multiple range test
- DMSO:
-
dimethyl sulphoxide
- DPPH:
-
1,1-diphenyl-2-picryl-hydrazyl
- EDTA:
-
ethylene diamine tetra-acetic acid
- ESI:
-
electrospray ionization
- Fr.:
-
fraction
- HPLC:
-
high performance liquid chromatography
- IR:
-
infra red
- LC-MS:
-
liquid chromatography-mass spectrometry
- MBC:
-
minimum bactericidal concentration
- MIC:
-
minimum inhibitory concentration
- MTT:
-
3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide
- NADH:
-
nicotinamide adenine dinucleotide
- NBCS:
-
newborn calf serum
- NBT:
-
nitroblue tetrazolium
- NMR:
-
nuclear magnetic resonance
- OD:
-
optical density
- PMS:
-
phenazine methosulphate
- PPP:
-
platelet-poor plasma
- PRP:
-
platelet-rich plasma
- SRB:
-
sulphorhodamine B
- TBA:
-
thiobarbituric acid
- TCA:
-
trichloroacetic acid
- TLC:
-
thin-layer chromatography
- UV:
-
ultraviolet
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Policegoudra, R.S., Rehna, K., Jaganmohan Rao, L. et al. Antimicrobial, antioxidant, cytotoxicity and platelet aggregation inhibitory activity of a novel molecule isolated and characterized from mango ginger (Curcuma amada Roxb.) rhizome. J Biosci 35, 231–240 (2010). https://doi.org/10.1007/s12038-010-0027-1
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DOI: https://doi.org/10.1007/s12038-010-0027-1