Abstract
Protease enzyme production by Bacillus licheniformis bacteria was investigated. Various agricultural wastes as substrate such as wheat bran, rice bran, and sugarcane bagasse were considered. The most important effective parameters on enzyme production, like incubation time, various substrates and solid substrate particle size, media pH, different nitrogen sources in a bench-scale designed bioreactor, were optimized. The optimum protease production conditions, for both Erlenmeyer flask and batch bioreactor, at 37 °C, pH of 8, incubation time of 48h, wheat bran (5 wt%) with the particle size of 1 mm, an equal amount of peptone and yeast extract (1% w/w) and agitation rate of 180 rpm were defined. In addition, maximum protease activity in the Erlenmeyer flask and batch bioreactor was 596 and 683.93 U/mL, respectively. The pH and thermal stability of produced protease were studied; the highest amount of remaining activities at pH 8 and 60 °C were 97 and 63% of initial activities, respectively. Also, shelf-life of the produced protease enzyme retained up to 88% of its initial activity after 30 days of storage at 4 °C. However, the produced enzyme was exposed remarkably compatible with the commercial detergent; the enzyme perfectly washed and removed the stains from the sample cotton textile.
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B. Asha and M. Palaniswamy, J. Appl. Pharm. Sci., 8, 119 (2018).
F. J. Contesini, R. R. d. Melo and H. H. Sato, Crit. Rev. Biotechnol., 38, 321 (2018).
A. Hakim, F. R. Bhuiyan, A. Iqbal, T. H. Emon, J. Ahmed and A. K. Azad, Heliyon, 4, e00646 (2018).
A. Razzaq, S. Shamsi, A. Ali, Q. Ali, M. Sajjad, A. Malik and M. Ashraf, Front. Bioeng. Biotechnol., 7, 110 (2019).
I. Talhi, L. Dehimat, A. Jaouani, R. Cherfia, M. Berkani, F. Almomani, Y. Vasseghian and N. K. Chaouche, Chemosphere, 286, 131479 (2022).
R. Hadjidj, A. Badis, S. Mechri, K. Eddouaouda, L. Khelouia, R. Annane, M. El Hattab and B. Jaouadi, Int. J. Biol. Macromol., 114, 1033 (2018).
J. G. dos Santos Aguilar and H. H. Sato, Food Res. Int., 103, 253 (2018).
M. B. Rao, A. M. Tanksale, M. S. Ghatge and V. V. Deshpande, Microbiol. Mol. Biol. Rev., 62, 597 (1998).
M. A. Emran, S. A. Ismail and A. M. Hashem, Biocatal. Agric. Biotechnol., 26, 101631 (2020).
D. Agrawal, P. Patidar, T. Banerjee and S. Patil, Process Biochem., 39, 977 (2004).
K. M. Sharma, R. Kumar, S. Panwar and A. Kumar, J. Genet. Eng. Biotechnol., 15, 115 (2017).
M. Sharma, Y. Gat, S. Arya, V. Kumar, A. Panghal and A. Kumar, Ind. Biotechnol., 15, 69 (2019).
H. Rekik, N. Z. Jaouadi, F. Gargouri, W. Bejar, F. Frikha, N. Jmal, S. Bejar and B. Jaouadi, Int. J. Biol. Macromol., 121, 1227 (2019).
V. F. Soares, L. R. Castilho, E. P. Bon and D. M. Freire, Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. (2005).
A. I. Adetunji and A. O. Olaniran, Biocatal. Agric. Biotechnol., 24, 101528 (2020).
V. Singh, S. Haque, R. Niwas, A. Srivastava, M. Pasupuleti and C. Tripathi, Front. Microbiol., 7, 2087 (2017).
S. Singh and B. K. Bajaj, Waste Biomass Valorization, 8, 453 (2017).
R. Potumarthi, S. Ch and A. Jetty, Biochem. Eng. J., 34, 185 (2007).
U. C. Banerjee, R. K. Sani, W. Azmi and R. Soni, Process Biochem., 35, 213 (1999).
R. Bergkvist, Acta Chem. Scand., 17, 8 (1963).
F. Bashir, M. Asgher, F. Hussain and M. A. Randhawa, Int. J. Biol. Macromol., 113, 944 (2018).
M. M. Bradford, Anal. Biochem., 72, 248 (1976).
A. Sellami-Kamoun, A. Haddar, N. E.-H. Ali, B. Ghorbel-Frikha, S. Kanoun and M. Nasri, Microbiol. Res., 163, 299 (2008).
J.-K. Yang, L. Shih, Y.-M. Tzeng and S.-L. Wang, Enzyme Microb. Technol., 26, 406 (2000).
P. Deb, S. A. Talukdar, K. Mohsina, P. K. Sarker and S. A. Sayem, Springerplus, 2, 1 (2013).
F. M. Olajuyigbe and J. O. Ajele, Afr. J. Biochem. Res., 2, 206 (2008).
I. Ahmed, M. A. Zia, T. Iftikhar and H. M. Iqbal, BioResources, 6, 4505 (2011).
F. Uyar and Z. Baysal, Process Biochem., 39, 1893 (2004).
A. Sharan and N. S. Darmwal, Bioresour. Technol., 98, 881 (2007).
H. Mukhtar and I. Haq, Sci. World J., 2013, Article ID 538067 (2013).
A. Sumantha, P. Deepa, C. Sandhya, G. Szakacs, C. R. Soccol and A. Pandey, Braz. Arch. Biol. Technol., 49, 843 (2006).
R. N. Z. R. Abd Rahman, L. P. Geok, M. Basri and A. B. Salleh, Enzyme Microb. Technol., 36, 749 (2005).
S. Gilani, G. Najafpour, H. Heydarzadeh and H. Zare, Chem. Ind. Chem. Eng. Q., 17, 179 (2011).
K. Jellouli, O. Ghorbel-Bellaaj, H. B. Ayed, L. Manni, R. Agrebi and M. Nasri, Process Biochem., 46, 1248 (2011).
A. Haddar, R. Agrebi, A. Bougatef, N. Hmidet, A. Sellami-Kamoun and M. Nasri, Bioresour. Technol., 100, 3366 (2009).
F. Abidi, F. Limam and M. M. Nejib, Process Biochem., 43, 1202 (2008).
Acknowledgements
The authors wish to acknowledge Biotechnology Research Center, Babol Noshirvani University of Technology (Iran) for the facilities provided to accomplish the present work. Also, this research did not receive any specific grant from funding agencies of the public, commercial, or from any non-profit organizations.
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Espoui, A.H., Larimi, S.G. & Darzi, G.N. Optimization of protease production process using bran waste using Bacillus licheniformis. Korean J. Chem. Eng. 39, 674–683 (2022). https://doi.org/10.1007/s11814-021-0965-3
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DOI: https://doi.org/10.1007/s11814-021-0965-3