Abstract
To evaluate the influence of various Cr(VI) concentrations (0.05, 0.25, 0.50, 1.00 and 2.00 g/kg) on the activity of soil enzymes, the activities of catalase, polyphenol oxidase, dehydrogenase, alkaline phosphatase in soils were investigated in the incubation experiment with a period of 35 d. The results indicate that all the tested Cr(VI) concentrations significantly inhibit dehydrogenase activity by over 70% after 35 d. The activity of alkaline phosphatase is slightly inhibited during the whole experiment except for on the day 7. Cr(VI) has no obvious effect on the activity of catalase in soil. On the contrary, Cr(VI) stimulates the activity of polyphenol oxidase. The results suggest that dehydrogenase activity can be used as an indicator for assessing the severity of chromium pollution.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
OBBARD J P. Ecotoxicological assessment of heavy metals in sewage sludge amended soils [J]. Applied Geochemistry, 2001, 16(11/12): 1405–1411.
HUANG Shun-hong, YANG Zhi-hui, CHAI Li-yuan, PENG Bing, SHAO Bo-hua. Toxicity of metal chromium to microorganism activities in soil around chromium-containing slag heap [J]. Journal of Central South University: Science and Technology, 2009, 40(1): 25–30. (in Chinese)
BANERJEE M, MISHRA S, CHATTERJEE J. Scavenging of nickel and chromium toxicity in Aulosira fertilissima by immobilization: Effect on nitrogen assimilating enzymes [J]. Electronic Journal of Biotechnology, 2004, 7(3): 302–309.
HUANG Shun-hong, PENG Bing, YANG Zhi-hui, CHAI Li-yuan, ZHOU Li-cheng. Chromium accumulation, microorganism population and enzyme activities in soils around chromium-containing slag heap of steel alloy factory [J]. Transaction of Non-ferrous Metals Society of China, 2009, 19(1): 241–248.
MEGHARAJ M, AVUDAINAYAGAM S, NAIDU R. Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste [J]. Current Microbiology, 2003, 47(1): 51–54.
GIANFREDA L. Activity of free and immobilized urease in soil: Effects of pesticides [J]. Soil Biology and Biochemistry, 1994, 26(6): 777–784.
DENG S P, TABATABAI M A. Cellulase activity of soils [J]. Soil Biology and Biochemistry, 1994, 26(10): 1347–1354.
RODRIGUEZ B B, BOLBOT J A, TOTHILLI E. Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples [J]. Biosensors and Bioelectronics, 2004, 19(10): 1157–1167.
HINOJOSA M B. Soil moisture pre-treatment effects on enzyme activities as indicators of heavy metal-contaminated and reclaimed soils [J]. Soil Biology and Biochemistry, 2004, 36(10): 1559–1568.
KIZILKAYA R. Dehydrogenase activity in Lumbricus terrestris casts and surrounding soil affected by addition of different organic wastes and Zn [J]. Bioresource Technology, 2008, 99(5): 946–953.
BELYAEVA O N, HAYNES R J, BIRUKOVA O A. Barley yield and soil microbial and enzyme activities as affected by contamination of two soils with lead, zinc or copper [J]. Biology and Fertility of Soils, 2005, 41(2): 85–94.
KHAN S, CAO Q, HESHAM A E L, XIA Y, HE J Z. Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb [J]. Journal of Environmental Sciences, 2007, 19(7): 834–840.
DUNGAN R S, KUKIER U, LEE B. Blending foundry sands with soil: Effect on dehydrogenase activity [J]. Science of the Total Environment, 2006, 357(1/3): 221–230.
MAJER B J, TSCHERKO D, PASCHKE A, WENNRICH R, KUNDI M, KANDELER E, KNASMULLER S. Effects of heavy metal contamination of soils on micronucleus induction in Tradescantia and on microbial enzyme activities: A comparative investigation [J]. Mutation Research — Genetic Toxicology and Environmental Mutagenesis, 2002, 515(1/2): 111–124.
ALEF K. Enzyme activities, in methods in applied soil microbiology and biochemistry [M]. London: Academic Press, 1995: 311–373.
VITI C, MINI A, RANALLI G, LUSTRATO G, GIOVANNETTI L. Response of microbial communities to different doses of chromate in soil microcosms [J]. Applied Soil Ecology, 2006, 34(2/3): 125–139.
VIG K, MEGHARAJ M, SETHUNATHAN N, NAIDU R. Bioavailability and toxicity of cadmium to microorganisms and their activities in soil: a review [J]. Advances in Environmental Research, 2003, 8(1): 121–135
WANG Y P, SHI J Y, WANG H, QI L, CHEN X C, CHEN Y X. The influence of soil heavy metals pollution on soil microbial biomass, enzyme activity, and community composition near a copper smelter [J]. Ecotoxicology and Environmental Safety, 2007, 67(1): 75–81.
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: Projects(2006AA06Z374, 2007AA021304) supported by the National High-Tech Research and Development Program of China; Project(2008SK2007) supported by the Key Program of Science and Technology of Hunan Province, China
Rights and permissions
About this article
Cite this article
Peng, B., Huang, Sh., Yang, Zh. et al. Inhibitory effect of Cr(VI) on activities of soil enzymes. J. Cent. South Univ. Technol. 16, 594–598 (2009). https://doi.org/10.1007/s11771-009-0099-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11771-009-0099-y