Abstract
The antioxidant activities of several extracts from Susabinori (Porphyra yezoensis) were measured by the ferric thiocyanate method and the thiobarbituric acid method. The methanol, acetone, ethyl acetate, and hexane extracts, and the chloroform-soluble and water-soluble fractions from the chloroform-methanol extract exhibited higher activities than α-tocopherol. The hot water extract showed little activity. Thin-layer chromatography analysis of the active extracts suggested the existence of several antioxidants. The activity of the chloroform soluble fraction was due to chlorophyll analogs. A strong antioxidant was isolated from the methanol extract, accompanied by several amino acids such as leucine and phenylalanine. This compound was identified as usujilene, a kind of mycosporine-glycine like amino acid.
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Yoshie, Y., T. Suzuki, T. Shirai, and T. Hirano, Free Amino Acids and Fatty Acid Composition in Dried Nori of Various Culture Locations and Prices, Bull. Japan Soc. Sci. Fish. 59:1769–1775 (1993).
Fujimoto, K., and T. Kaneda, Screening Test for Antioxygenic Compounds from Marine Algae and Fractionation from Eisena bicyclis and Undaria pinnatifida, Ibid.:1125–1130 (1980).
Tutour, B.L., Antioxidative Activities of Algal Extracts, Synergistic Effect with Vitamin E, Phytochemistry 29:3759–3765 (1990).
Nishibori, S., and K. Namiki, Autoxidative Substances in Green Fractions of the Lipids of Aonori (Enteromorpha sp.), J. Home Econ. Jpn. 39:1173–1178 (1988).
Kikuzaki, H., and N. Nakatari, Antioxidant Effects of Some Ginger Constituents, J. Food Sci. 58:1407–1410 (1993).
Omata, T., and N. Murata, Preparative of Chlorophyll a, Chlorophyll b and Bacterochlorophyll a by Column Chromatography with DEAE-Sepharose CL-6B and Sepharose CL, Plant Cell Physiol. 24:1093–1100 (1983).
Araki, S., T. Oohusa, T. Omata, and N. Murata, Column Chromatographic Separation of Chlorophyllide a and Pheophorbide a, Ibid.:841–843 (1984).
Heinonen, M., K. Haila, A.M. Lampi, and V. Piironen, Inhibition of Oxidation in 10% Oil-in-Water Emulsions by β-Carotene with α- and γ-Tocopherols, J. Am. Oil Chem. Soc. 74:1047–1052 (1997).
Endo, Y., R. Usuki, and T. Kaneda, Antioxidant Effect of Chlorophyll and Pheophytin on the Autoxidation of Oils in the Dark. I. Comparison of the Inhibitory Effects, Ibid.:1375–1378 (1985).
Merck, E., Dyeing Reagents for Thin Layer and Paper Chromatography, Darmstadt, (1980), pp. 12, 35, 51, 61.
Müller, L., Sensitivity Enhanced Detection of Weak Nuclei Using Heteronuclear Multiple Quantum Coherence, J. Am. Chem. Soc. 101:4481–4484 (1979).
Bax, A., and M.F. Summers, 1H and 13C Assignments from Sensitivity-Enhanced Detection of Heteronuclear Multiple-Bond Connectivity by 2D Multiple Quantum NMR, Ibid.:2093–2094 (1986).
Sekikawa, I., C. Kubota, T. Hiraoki, and I. Tujino, Isolation and Structure of a 357 nm UV-Absorbing Substance, Usujirene, from the Red Alga Palmaria palmata (L.) O. Kuntze, Jpn. J. Phycol. 34:185–188 (1986).
Yoshida, T., and P.M. Sivalingam, Isolation and Characterization of the 337 µm UV-Absorbing Substance in Red Alga, Porphyra yezoensis Ueda, Plant Cell Physiol. 11:427–434 (1979).
Sivalingam, P.M., T. Ikawa, and K. Nishizawa, Isolation and Physico-Chemical Properties of a Substance 334 from the Red Alga, Porphyra yezoensis Ueda, Bot. Mar. 19:1–7 (1976).
Ito, S., and Y. Hirata, Isolation and Structure of a Mycosporine from the Zoanthid Palythoa tuberculosa, Tetrahedron Lett. 28:2429–2430 (1977).
Takano, S., D. Uemura, and Y. Hirata, Isolation and Structure of Two New Amino Acids, Palythinol and Palythene, from the Zoanthid Palythoa tuberculosa, Ibid.:4909–4912 (1978).
Takano, S., A. Nakanishi, D. Uemura, and Y. Hirata, Isolation and Structure of a 334 nm-UV-Absorbing Substance, Porphyra-334 from the Red Alga Porphyra tenera Kjellman, Chem. Lett.: 419–420 (1979).
Bandaranayake, W.M., J.E. Bemis, and D.J. Bourne, Ultraviolet Absorbing Pigments from the Marine Sponge Dysidea herbacea: Isolation and Structure of a New Mycosporine, Comp. Biochem. Physiol. 115:281–286 (1996).
Carreto, J.I., M.O. Carignan, G. Daleo, and S.G. Marco, Occurrence of Mycosporine-Like Amino Acids in the Red-Tide Dinoflagellate Alexandrium excavatum: UV-Photoprotective Compounds? J. Plankton Res. 12:909–921 (1990).
Sivalingam, P.M., T. Ikawa, Y. Yokohama, and K. Nishizawa, Distribution of a 334 UV-Absorbing Substance in Algae with Special Regard of Its Possible Physiological Roles, Bot. Mar. 17:23–29 (1974).
Dunlap, W.C., and Y. Yamamoto, Small-Molecule Antioxidants in Marine Organism: Antioxidant Activity of Mycosporine-Glycine, Comp. Biochem. Physiol. 112:105–114 (1995).
Frankel, E.N., Recent Advances in Lipid Oxidation, J. Sci. Food Agric. 54:495–511 (1991).
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Nakayama, R., Tamura, Y., Kikuzaki, H. et al. Antioxidant effect of the constituents of susabinori (Porphyra yezoensis) . J Amer Oil Chem Soc 76, 649–653 (1999). https://doi.org/10.1007/s11746-999-0017-3
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DOI: https://doi.org/10.1007/s11746-999-0017-3