Abstract
High-resolution nuclear magnetic resonance (NMR) spectroscopy provides useful data for analyzing fatty acid compositions of edible vegetable oils. Quantitation of each fatty acid was carried out by evaluation of particular peaks. According to the 1H NMR method, terminal methyl protons, divinyl protons, and allyl protons are useful to calculate linolenic acid, linoleic acid, and oleic acid, respectively. The ω-2 carbon, divinyl carbon, and allylic carbons were used for calculation of these acids by the 13C NMR method. Compositional results obtained by NMR coincided well with those of the conventional gas chromatography (GC) method. Results from 13C NMR were in better agreement with those from GC than were the results obtained by the 1H NMR method.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Craske, J.D., Separation of Instrumental and Chemical Errors in the Analysis of Oils by Gas Chromatography—A Collaborative Evaluation, J. Am. Oil Chem. Soc. 70:325–334 (1993).
Watanabe, S., T. Nagai, S. Hayano, K. Ikeda, A. Kato, K. Kunihiro, T. Murui, and M. Negishi, Determination of Fatty Acid Composition by Programmed Temperature Gas Chromatography, Yukagaku 24:588–594 (1975).
Shoolery, J.N., Applications of High Resolution Nuclear Magnetic Resonance to Study of Lipids, in Dietary Fats and Health, edited by E.G. Perkins, American Oil Chemists’ Society, Champaign, 1983, pp. 220–240.
Sacchi, R., T. Medina, S.P. Aubourg, F. Addeo, and L. Palillo, Proton Nuclear Magnetic Resonance Rapid and Structure-Specific Determination of ς-3 Polyunsaturated Fatty Acids in Fish Lipids, J. Am. Oil Chem. Soc. 70:225–228 (1993).
Aursand, M., L. Jorgensen, H. Grasdalen, Positional Distribution of ɛ-3 Fatty Acids in Marine Lipids Triacylglycerols by High-Resolution 13C Nuclear Magnetic Resonance Spectroscopy, Ibid.:293–297 (1995).
Diehlund, B.W.K., and W. Ockels, Ermittelung der Fettsäureverteilung Durch 13C-NMR Spectroskopie, Fat Sci. Technol. 97:115–118 (1995).
Matsui, T., H. Iwasaki, F. Matsumoto, and J. Osajima, Quality Evaluation of Edible Oils by Proton Nuclear Magnetic Resonance Measurement, Food Sci. Technol. Int. 1:94–97 (1995).
Gunstone, F.D., The Composition of Hydrogenated Fats by High-Resolution 13C Nuclear Magnetic Resonance Spectroscopy, J. Am. Oil Chem. Soc. 70:965–970 (1993).
Wanasundara, U.N., and F. Shahidi, Application of NMR Spectroscopy to Assess Oxidative Stability of Canola and Soybean Oils, J. Food Lipids 1:15–24 (1993).
Gunstone, F.D., High Resolution 13C NMR. A Technique for the Study of Lipid Structure and Composition, Prog. Lipid Res. 33:19–28 (1994).
Miyake, Y., K. Yokomizo, and N. Matsuzaki, Rapid Determination of Iodine Value by 1H Nuclear Magnetic Resonance Spectroscopy, J. Am. Oil Chem. Soc. 75:15–19 (1998).
Official Methods and Recommended Practices of the American Oil Chemists’ Society, 4th edn., Amsterdam Oil Chemists’ Society, Champaign, 1990.
Author information
Authors and Affiliations
About this article
Cite this article
Miyake, Y., Yokomizo, K. & Matsuzaki, N. Determination of unsaturated fatty acid composition by high-resolution nuclear magnetic resonance spectroscopy. J Amer Oil Chem Soc 75, 1091–1094 (1998). https://doi.org/10.1007/s11746-998-0295-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11746-998-0295-1