Abstract
Trichoderma sp. AM076, isolated from a freshwater sample, was found to accumulate 9,12-cis-hexadecadienoic acid (16:2ω4), when grown with palmitoleic acid (16: 1ω7). Methyl myristate was the best carbon source for the conversion of palmitoleic acid to 16:2ω4. The mycelial 16:2ω4 content reached 17.4 mg/g dry mycelia (443 mg/L) when the fungus was grown in a medium that contained 2.0% methyl myristate, 1.5% yeast extract, and 2.0% methyl palmitoleate, pH 6.0, for 5 d at 28°C with shaking. In both nonpolar and polar lipids from the mycelia, 16:2ω4 was detected as one of the major fatty acids when 16:1ω7 was added. It is probable that 16:1ω7 is converted to 16:2ω4 through the Δ12 desaturation reaction.
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References
Chuecas, L., and J.P. Riley, Component Fatty Acids of the Total Lipids of Some Marine Phytoplankton, J. Mar. Biol. Ass. U.K. 49:97–116 (1969).
Ackman, R.G., P.M. Jangaard, R.J. Hoyle, and H. Brocherhoff, Origin of Marine Fatty Acids. I. Analysis of the Fatty Acids Produced by the Diatom Skeletonema costatum, J. Fish. Res. Bd. Canada 21:747–756 (1964).
Ackman, R.G., C.S. Tocher, and J. Mclachlan, Marine Phytoplankton Fatty Acids, Ibid.:1603–1620 (1968).
Nichols, B.W., and R.S. Appleby, The Distribution and Biosynthesis of Arachidonic Acid in Algae, Phytochemistry 8:1907–1915 (1969).
Orcutt, D.M., and G.M. Patterson, Sterol, Fatty Acid and Elemental Composition of Diatom Grown in Chemical Defined Media, Comp. Biochem. Physiol. 50:579–583 (1975).
Shimizu, S., H. Kawashima, Y. Shinmen, K. Akimoto, and H. Yamada, Production of Odd Chain Polyunsaturated Fatty Acids by Mortierella Fungi, J. Am. Oil Chem. Soc. 68:254–258 (1991).
Shirasaka, N., T. Yokochi, and S. Shimizu, Formation of a Novel Odd Chain Polyunsaturated Fatty Acid, 5,8,11,14,17-cis-Nonadecapentaenoic Acid, by an EPA-Producing Aquatic Fungus, Sprolegnia sp. 28YTF-1, Biosci. Biotech. Biochem. 59:1963–1965 (1995).
Bligh, E.G., and W.J. Dyer, A Rapid Method of Total Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37:911–917 (1959).
Christie, W.W., E.Y. Brechany, S.B. Jonson, and R.T. Holman, A Comparison of Pyrrolidide and Picolinyl Ester Derivatives for the Identification of Fatty Acids in Natural Samples by Gas Chromatography-Mass Spectrometry, Lipids 21:657–661 (1986).
Domsch, K.H., W. Gams, and T.H. Anderson, in Compendium of Soil Fungi, Academic Press, London, Vol. 1, 1980, p. 859.
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Shirasaka, N., Umehara, T., Murakami, T. et al. Microbial conversion of palmitoleic acid to 9,12-hexadecadienoic acid (16:2ω4) by Trichoderma sp. AM076. J Amer Oil Chem Soc 75, 717–720 (1998). https://doi.org/10.1007/s11746-998-0211-8
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DOI: https://doi.org/10.1007/s11746-998-0211-8