Abstract
The biodegradabilities of different oil-based fatliquors derived from rape oil, fish oil, castor oil or mineral oil variants were investigated by evaluating the respiration curves, BOD5/COD values, COD (chemical oxygen demand) and TOC (total organic carbon) removal ratios. Simultaneously, degradation kinetics of the fatliquors were also studied. The results indicated that the BOD5/COD values and the COD and TOC removal ratios of all the natural oil based products are higher than 0.45 and 85%, respectively, implying that all of them are biodegradable. The mineral oil based fatliquors have lower than 0.2 and 10% values, showing unbiodegradable characteristics and were used as the control. The biodegradability order is castor oil > fish oil > rape oil > mineral oil product. Further study indicated that the differences in biodegradability result from the varying fatty acid composition (such as ricinoleic acid and polyunsaturated fatty acids). The higher the active group content, the more beneficial for modification reactions and result in a higher biodegradation rate. The degradation kinetics studies revealed that the degradation rate constants (k) of castor oil, fish oil and rape oil products are 0.87, 0.84 and 0.81 d−1 for the sulfated fatliquor, and 0.95, 0.93, 0.85 d−1 for the oxidized–sulfited fatliquors, respectively; indicating that the overall degradation rate followed the same trend as the biodegradability order where castor oil > fish oil > rape oil, whether the fatliquors underwent modification as sulfated or oxidized–sulfited.
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Introduction
It is well known that the fossil raw materials are irrevocably decreasing and the pressure on the environment is building up. Thus the progressive trend that the chemical industry is turning to renewable raw materials, emerges as an inevitable necessity [1]. Natural oils have attracted renewed attention as raw materials for the preparation of various materials, to replace or augment the traditional petrochemical based materials. Triglycerides, which are composed of three fatty acids connected by a glycerol center, are the main component of natural oils, such as rape oil, fish oil and castor oil, etc. There are numerous ways of chemically modifying the unsaturated sites on the fatty acids and different applications in industry. In addition to their application in the food industry, triglyceride oils have been used for the production of coatings, inks, plasticizers, lubricants, agrochemicals, bitumen flux and leather fatliquors [2–4].
Fatliquoring is one of the key operations in the leather manufacturing [5]. It is an oil-addition process by which the leather fibers are lubricated so that after drying they are capable of slipping over one another and producing an adequate compliance and softness [6]. Natural oil (such as rape oil, fish oil and castor oil) and mineral oil based fatliquors are the two kind of widely applied products in the leather industry. Usually fatliquors are used in excess to ensure full penetration and complete reaction with leather fibers. As a result, unabsorbed fatliquors inevitably remain in the float, generating a high level of pollution. Moreover, it is the largest amount of chemicals (10–20 wt%, based on the weight of wet blue leather) used in the leather-making. The biodegradability of fatliquors is therefore considered as one of the most important factors associated in evaluating the environmental friendliness of a process in the leather industry.
Biodegradability of chemicals depends not only on the molecular structure of the tested compound but also on the availability of microorganism accessibility of metabolic cofactors (i.e., O2, nutrients, etc.), growth medium and other environmental conditions, such as temperature and humidity [7]. However, the biomass, metabolic cofactors, growth medium and environmental conditions can be controlled with a standardized method. Therefore, the structure parameter, involving the main chain structure, substituted groups, polarity and active groups, is the key issue affecting the biodegradation. Knowing the relationship between the molecular structure and biodegradation of fatliquors could not only complement and substitute in part for some of the costly experimental evaluation of biodegradability but also help to identify and potentially avoid the production of new chemical compounds which are not easy to biodegrade. Thus, it would support the development of environmentally sustainable new products and the design of synthesis strategies if poorly degradable intermediates and waste products can be avoided [8, 9].
In our previous work, the rape oil was modified with different methods, such as sulfated, sulfonated, oxidized–sulfited, phosphated and copolymeric reactions. The effects of different modification methods on the biodegradability of rape oil-based fatliquors were investigated, and showed a biodegradability order of phosphated > sulfonated > oxidized–sulfited > sulfated > copolymeric [10]. The modification method which consumed the hydroxyl groups and double bonds, such as sulfated modification would decrease the biodegradability in 5 days. The others which did not consume those active groups, such as sulfonated and oxidized–sulfited, showed better biodegradability. In the polymerization method, the double bonds are consumed or modified, but due to the resulting larger molecular size and steric effect, the biodegradability is decreased [10]. In the current study, the different oils (rape, fish and castor oils) were modified uniformly (sulfated or oxidized–sulfited) and the difference in their biodegradabilities and degradation kinetics were investigated in detail by using mineral oil-based fatliquors (alkyl sulfonyl chloride) as a reference. The purpose of this study was to investigate how the structure parameters of the original oils influence the biodegradabilities of the modified products and to provide guidance for developing environmental-friendly fatliquors.
Experimental Procedures
Materials
Rape oil, fish oil and castor oil were obtained from Tingjiang Chemical Co. (Sichuan, China). All chemicals used for synthesis were of laboratory grade, while the chemicals used for analysis were of analytical grade.
Preparation of Different Oil-Based Fatliquors
The different oil-based fatliquors were prepared by sulfate and oxidized–sulfited reactions according to references [11, 12]. The reaction principles are shown in Fig. 1.
Characterizations
Fatty acid composition was determined by gas chromatography–mass spectrometry (GC–MS) (GC–MS TraceDSQII; ThermoFisher, USA) under the following conditions: oil transesterification to methyl esters; DB-5 capillary column 30 m × 0.25 mm i.d.; helium as carrier gas (40 kPa pressure); air pressure 100 kPa; hydrogen pressure 50 kPa; injection on column; flame-ionization detection at 220 °C and ionization energy of 70 eV; programmed oven temperature from 80 to 260 °C at 5 °C/min.
Iodine values were determined according to the ISO 3961 standard method. Whereas, the AOCS standard method Cd 13-60 was used to determine the hydroxyl values (expressed in mg of KOH per g of oil) [13].
Biodegradation was determined under aerobic conditions. Activated sludge from the aeration basin of a wastewater treatment plant was used as the microbial biomass for the test. Before use in the test, the sludge was washed twice with tap water and starved under aeration for 24 h. The concentration of the activated sludge was determined [14] and expressed as mixed liquid suspended solids (MLSS). The pH of the activated sludge was adjusted to 6.8–7.2 using a hydrochloric acid solution (1 M HCl) or a sodium hydroxide solution (1 M NaOH). In the test, the fatliquors were added to a mineral medium (Ingredients [15]: KH2PO4 1 g/L, KNO3 0.5 g/L, MgSO4·7H2O 0.1 g/L, CaCl2 0.1 g/L, FeCl3 0.01 g/L, NaCl 1 g/L) as the sole source of carbon, and the sealed vessels with a headspace of air were inoculated with activated sludge (suspended solids 4 g/L). The tests were run for 5 days at 20 °C with continuous shaking. Biodegradation was monitored by plotting the biological respiration curve, BOD5/COD value, as well as COD and TOC removal ratios.
The COD and BOD (biochemical oxygen demand) were measured by using Hanna HI 99721 and HI 99724A-6 equipment, respectively (Hanna Instruments, Italy). The data are averages of three separate measurements. The COD removal ratio is defined as:
where COD0 is the original chemical oxygen demand of the test sample solution (mg/L), and COD5 is the chemical oxygen demand after the solution has biodegraded in 5 days.
TOC analyses were done using an Anatel TOC-2000 TOC analyzer (Shimadzu, Japan). The data are averages of three separate measurements. The TOC removal ratio is defined as:
where TOC0 is the initial and TOC5 is the final chemical oxygen demand of the test sample solution (mg/L) after biodegradation in 5 days.
Results and Discussion
Analysis of Fatty Acid Composition of the Oils
Rape oil, castor oil and fish oil are typical natural renewable oils, and mineral oil is a representative of non-renewable fossil oil and are widely used in the leather industry as fatliquors. Rape oil, castor oil or fish oil based sulfated or oxidized–sulfited fatliquors, as well as the alkyl sulfonyl chloride fatliquors are frequently used natural or mineral oil based fatliquors, respectively. The fatty acid composition (summarized in Table 1) differ in each oil sample. Table 2 lists the statistical saturated, monounsaturated and polyunsaturated fatty acids components. As shown in Tables 1, 2, castor oil, with huge amount of ricinoleic acid, has the lowest amount of saturated fatty acids. The ricinoleic acid (Fig. 2) contains hydroxyl group on the molecular backbone which is not present in other oil. The polyunsaturated fatty acids [in the form of EPA, DHA and heneicosapentanoic acid (Fig. 2)] composition in fish oil is about 33%. The monounsaturated fatty acids in rape oil are the majority and polyunsaturated fatty acids existed only in the form of biunsaturated or triunsaturated fatty acids. The mineral oil is composed primarily of saturated hydrocarbon, with minor double bonds and no hydroxyl groups.
The Biodegradabilities of Different Oil-Based Sulfated Fatliquors
The activated sludge process is an efficient and widely used method in wastewater treatment [16]. Respiration is the essential activity of aerobic microorganisms in the activated sludge. The respiration of the activated sludge will be different from its endogenous respiration when there are chemicals in wastewater. Thus, the biodegradability of the fatliquors can be qualitatively evaluated by the difference in biological respiration curves [17] (the respiration curve is the curve that the BOD change with time). When a biodegradable chemical is utilized as a carbon and energy source for the organism growth in activated sludge, the respiration of activated sludge will be enhanced; In contrast, if the chemical is toxic to the microorganisms of the activated sludge, the respiration will be inhibited. The respiration curves of rape oil, fish oil, castor oil based sulfated fatliquors and mineral oil-based fatliquors (alkyl sulfonyl chloride) as well as the endogenous respiration curve of the activated sludge are shown in Fig. 3.
The respiration curves of activated sludge in the presence of the three natural oil-based fatliquors individually, are all above the endogenous respiration curve, which means that all of them are biodegradable. And the respiration curve of activated sludge in the presence of castor oil based fatliquors is higher than fish and rape oil indicating that the biodegradability of the castor oil type fatliquors is the best, and the biodegradability of rape oil based fatliquors is not as good as other two. However, the respiration curve of the alkyl sulfonyl chloride is lower than the endogenous respiration curve, suggesting that the mineral oil based fatliquors could not be biodegraded by the activated sludge.
The biodegradability of an organic compound can also be evaluated by measuring the BOD5/COD values. The BOD5 is the amount of oxygen consumed by biochemical oxidation of waste contaminants over a 5-day period. A higher BOD5/COD ratio is associated with better biodegradability [18]. A compound is usually considered as an easily biodegradable one when its BOD5/COD value is higher than 0.45. On the contrary, it is considered as a hardly biodegradable one when the value is lower than 0.20 [18]. In Table 3, the BOD5/COD value is shown to be greater for castor oil based fatliquors than for other natural oil-based fatliquors but all have higher than 0.45 values. Whereas the alkyl sulfonyl chloride’s is lower than 0.2 ratio, indicating that all natural oil-based fatliquors are biodegradable, while the mineral oil fatliquors are not.
The biodegradability of chemicals determined by activated sludge respiration is based on the oxygen consumption of aerobic microorganisms. The oxygen consumption is characterized by the COD value and is commonly used as a crucial parameter to reflect total pollution content in the wastewater. However, due to the physical adsorption of the activated sludge to the chemicals, the COD value cannot solely reflect the biodegradation. Thus, biodegradation is further confirmed by the TOC removal ratio analysis. When the soluble carbon of the biodegradable chemicals in the wastewater is utilized as the source of carbon and energy for the growth of organisms in activated sludge, [19] it is gradually consumed by the organisms, resulting in a decrease of the TOC value after treating with activated sludge for 5 days.
As shown in Table 3, the TOC removal ratios of all the natural oil based fatliquors are higher than 85%, but the mineral oil fatliquors is lower than 10%, which is in agreement with the order of the COD removal ratios.
The difference in the biodegradabilities of these fatliquors can be attributed to different fatty acid composition and active groups’ content after modification. As mentioned in Fig. 1, the sulfated reaction mainly consumed double bonds and hydroxyl groups, so after modification the hydroxyl and iodine values were decreased for all samples as shown in Table 4. For castor oil the hydroxyl value is higher than the others while the iodine value is almost the same as the other products after modification. It is believed that due to their electron donating capability, the hydroxyl groups have played an important role in castor oil biodegradation.
For fish oil and rape oil based fatliquors, it is the double bond content that dominantly influence their biodegradation rather than the hydroxyl groups’ content. The iodine value in fish oil is higher than the rape oil, but after modification, the iodine values are almost the same in all oil samples. This can be attributed to the polyunsaturated fatty acids [such as EPA, DHA and heneicosapentanoic acid, see Fig. 3] content in the oils, the former is greater for the latter, and these non-conjugated double bonds are beneficial for both modification reactions and faster biodegradation. In rape oil, the double bonds existed mainly in the monounsaturated fatty acids, and the activity of these double bonds is less than the non-conjugated double bonds, hence the biodegradation by cleavage the double bond is slower than the polyunsaturated fatty acids; that is why the biodegradability of rape based fatliquors is inferior to the other two products.
However, in the mineral oil fatliquors, the major components are saturated hydrocarbon derivatives and no hydroxyl groups or double bonds are involved. The long chain alkyl groups (long C–C chains) are quite difficult to biodegrade, and the existence of the strong electron-withdrawing group (–Cl) further decrease its biodegradability.
The Biodegradabilities of Oxidized–Sulfited Fatliquors
Figure 4 displays the respiration curves of the oxidized–sulfited fatliquors and mineral oil based fatliquors, as well as the endogenous respiration curve of the activated sludge. It can be seen that the substrate respiratory curves of the natural oil fatliquors are all above the endogenous respiration curve, showing good biodegradabilities. On the contrary, the respiratory curves in presence of alkyl sulfonyl chloride is below the endogenous respiration curve which means the alkyl sulfonyl chloride can hardly be degraded by the microorganism.
Similar conclusions can be drawn from the analysis of BOD5/COD values, COD and TOC removal ratios. As shown in Table 3, the natural oil based oxidized–sulfited fatliquors are easily degraded whilst the mineral oil based fatliquors is hardly degraded. As shown in Table 4, the changes tendency of hydroxyl and iodine values for all oxidized–sulfited samples are the same for the sulfated fatliquors, so the biodegradability show the same order.
The Biodegradation Kinetics of Different Oil Based Fatliquors
The respirations, BOD5/COD values and COD, TOC removal ratios analysis can provide a possibility of biodegradation but cannot tell which one biodegrade faster or slower. Previous studies indicated that the BOD with time of low concentration organic compound under activated sludge treatment, approached to single-molecule reaction mechanism, and accorded with first-order reaction kinetics model [20].
Respiration rate is the oxygen consuming rate of the aerobic microorganisms that participate in the biodegradation. Assume that the ultimate BOD is L 0 (the total amount of oxygen consumed when the biochemical reaction is allowed to proceed to completion is called the ultimate BOD), and then at time t (d) the respiration rate is proportional to the residual BOD L t, and can be derived as Eqs. (1)–(3):
Thus,
where, L 0 represents the ultimate BOD (mg/L), and residual BOD L t is the total remaining BOD at time t (mg/L), that is subtracting the BOD of time t from ultimate BOD; y t is the BOD at time t (mg/L); k is the rate constant of BOD (1/d); t 0 is persists time (d).
This model contains three kinetics parameters which reflect the different biodegradabilities of these fatliquors. L 0/COD is the direct reflection of the degree of degradation; k describes degradation rate (the larger the k value is, the quicker the biodegradation reaction will be) and t 0 reflects the adaptability of the activated sludge with fatliquors, t 0 means the time when the biodegradation reaction start. The values for the model parameters after model optimization are shown in Table 5.
The degradation rate or k values of the tested fatliquors, either sulfated or oxidized–sulfited products, show a uniform order that castor oil > fish oil > rape oil. Biodegradation kinetics study also confirms the above conclusion, that is, the biodegradability of different oil-based fatliquors are mostly associated with the fatty acid composition and active groups (double bonds and hydroxyl groups) content in the oils, higher contents of them are beneficial for their biodegradation.
Conclusions
-
1.
These cumulative results indicate that the fatty acid composition is the most important factor associated with the biodegradabilities of different oil-based fatliquors. The presence of hydroxyl groups containing a fatty acid (ricinoleic acid) and non-conjugated polyunsaturated fatty acids, such as EPA, DHA and heneicosapentanoic acid are essential for both modification reactions and faster biodegradation.
-
2.
The active group content depends on the fatty acid composition. The fatty acid composition and the amount of the active groups such as the double bonds and hydroxyl groups can directly affect the biodegradabilities of these fatliquors. The higher the content of unsaturated fatty acids and hydroxyl groups, the faster is the biodegradability of fatliquors.
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3.
The biodegradability of natural oil based fatliquors is superior to mineral type, and generating the order of castor oil > fish oil > rape oil > mineral oil.
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4.
The overall degradation rate or k values of the tested fatliquors, either sulfated or oxidized–sulfited products, followed the same trend as the biodegradability order.
References
Lichtenthaler FW, Peters S (2004) Carbohydrates as green raw materials for the chemical industry. C. R. Chimie 7:65–90
Salunkhe DK, Chavan JK, Adsule RN, Kadam SS (1992) World oilseeds: chemistry, technology and utilization. Van Nostrand Reinhold, New York, pp 19–21
Metzger JO, Bornscheuer U (2006) Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification. Appl Microbiol Biotechnol 71:13–22
Niczke L, Czechowski F, Gawel I (2007) Oxidized rape oil methyl ester as a bitumen flux: structural changes in the ester during catalytic oxidation. Prog Org Coat 59:304–311
Luo ZY, Fan HJ, Lu Y (2008) Fluorine-containing aqueous copolymer emulsion for waterproof leather. J Soc Leath Tech Ch 92:107–113
Liu CK, Latona NP, Dimaio GL (2002) Physical property studies for leather lubricated with various types of fatliquors. J Am Leather Chem 97:431–440
Ahtiainen J, Aalto M, Pessala P (2003) Biodegradation of chemicals in a standardized test and in environmental conditions. Chemosphere 51:529–537
Boxall AB, Sinclair CJ, Fenner K (2004) When synthetic chemicals degrade in the environment. Environ Sci Technol 38:368–375
Philipp B, Hoff M, Germa F (2007) Biochemical interpretation of quantitative structure-activity relationships (QSAR) for biodegradation of N-Heterocycles: a complementary approach to predict biodegradability. Environ Sci Technol 41:1390–1398
Luo ZY, Yao J, Fan HJ (2010) The biodegradabilities of rape oil-based fatliquors prepared from different methods. J Am Leather Chem 105:121–128
Li GP (1997) The chemistry and application principle of leather chemicals. Chinese light industry press, Beijing, pp 193–195
Gao ZX, Qiang XH, Fan GD (2002) Study about new technological route of oxidated and oxidized–sulfited rape oil. China Leather 31:8–10
Official Methods and recommended practices of The Am Oil Chem Soc, 4th edn (1997) Am. Oil Chem. Soc., Champaign
APHA (1998) Standard methods for examination of water and wastewater, 20th edn. American Public Health Association, Port City Press, Baltimore
Luo ZY, Wang SS, Fan HJ (2010) A novel biodegradable fluorine-containing copolymer surfactant. J Polym Environ 18:339–345
Wang FY, Rudolph V, Zhu ZH (2008) Encyclopedia of Ecology, 3227-3242
Gendig C, Domogala G, Agnoli F (2003) Evaluation and further development of the activated sludge respiration inhibition test. Chemosphere 52:143–149
Cao JH, Huang CW (2001) A simple method of estimation of the dilution ratio in BOD5 determination. Phys Test Chemic Anal B Chemic Anal 37:206–209
Walski TM, Biga RB (1991) Case study in sludge handling. Proceedings of the 1991 Specialty Conference on Environmental Engineering 8: 667–672
Mao HZ, Smith DW (1995) Mechanistic model for assessing biodegradability of complex wastewaters. Water Res 29:1957–1975
Acknowledgments
The authors wish to acknowledge the financial support from the Hi-tech Research and Development Program of China (863 Program, Project Number: 2007AA03Z341) and National Science Foundation of China (Project Number: 20976110).
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Luo, Z., Xia, C., Fan, H. et al. The Biodegradabilities of Different Oil-Based Fatliquors. J Am Oil Chem Soc 88, 1029–1036 (2011). https://doi.org/10.1007/s11746-010-1749-9
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DOI: https://doi.org/10.1007/s11746-010-1749-9