Abstract
An analytical procedure was developed for regiodistribution analysis of TAG using α-MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative α-MAG, allowing the composition of the native TAG in the α-position to be determined directly. The composition in the β-position can then be estimated from the composition of the α-MAG and TAG according to the formula, 3×TAG-2×α-MAG. The estimates are superior to those obtained using the α,β-DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SD. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil.
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Abbreviations
- DCF:
-
2′,7′-dichlorofluorescein
- POP:
-
sn-1,3-dipalmitoleyl-sn-2-oleoyl-glycerol
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Turon, F., Bachain, P., Caro, Y. et al. A direct method for regiospecific analysis of TAG using α-MAG. Lipids 37, 817–821 (2002). https://doi.org/10.1007/s11745-002-0966-3
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DOI: https://doi.org/10.1007/s11745-002-0966-3