Abstract
Although fatty acid-binding proteins (FABP) differentially affect fatty acid uptake, nothing is known regarding their role(s) in determining cellular phospholipid levels and phospholipid fatty acid composition. The effects of liver (L)- and intestinal (I)-FABP expression on these parameters were determined using stably transfected L-cells. Expression of L- and I-FABP increased cellular total phospholipid mass (nmol/mg protein) 1.7- and 1.3-fold relative to controls, respectively. L-FABP expression increased the masses of choline glycerophospholipids (ChoGpl) 1.5-fold, phosphatidylserine (PtdSer) 5.6-fold, ethanolamine glycerophospholipids 1.4-fold, sphingomyelin 1.7-fold, and phosphatidylinositol 2.6-fold. In contrast, I-FABP expression only increased the masses of ChoGpl and PtdSer, 1.2- and 3.1-fold, respectively. Surprisingly, both L- and I-FABP expression increased ethanolamine plasmalogen mass 1.6- and 1.1-fold, respectively, while choline plasmalogen mass was increased 2.3- and 1.7-fold, respectively. The increase in phospholipid levels resulted in dramatic 48 and 33% decreases in the cholesterol-to-phospholipid ratio in L- and I-FABP expressing cells, respectively. L-FABP expression generally increased polyunsaturated fatty acids, primarily by increasing 20∶4n−6 and 22∶6n−3, while decreasing 18∶1n−9 and 16∶1n−7. I-FABP expression generally increased only 20∶4n−6 proportions. Hence, expression of both I- and L-FABP differentially affected phospholipid mass, class composition, and acyl chain composition. Although both proteins enhanced phospholipid synthesis, the effect of L-FABP was much greater, consistent with previous work suggesting that these two FABP differentially affect lipid metabolism.
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Abbreviations
- CerPCho:
-
sphingomyelin
- ChoGpl:
-
choline glycerophospholipid
- ER:
-
endoplasmic reticulum
- EtnGpl:
-
ethanolamine glycerophospholipid
- FABP:
-
fatty acid binding protein
- FAME:
-
fatty acid methyl ester
- GLC:
-
gas-liquid chromatography
- HPLC:
-
high-performance liquid chromatography
- I-FABP:
-
intestinal-FABP
- L-FABP:
-
liver-FABP
- MUFA:
-
monounsaturated fatty acid
- PlsCho:
-
choline plasmalogen
- PlsEtn:
-
ethanolamine plasmalogen
- PtdEtn:
-
phosphatidylethanolamine
- PtdIns:
-
phosphatidylinositol
- PtdOH:
-
phosphatidic acid
- PtdSer:
-
phosphatidylserine
- PUFA:
-
polyunsaturated fatty acid
- TLC:
-
thin-layer chromatography
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Murphy, E.J., Prows, D.R., Stiles, T. et al. Liver and intestinal fatty acid-binding protein expression increases phospholipid content and alters phospholipid fatty acid composition in L-cell fibroblasts. Lipids 35, 729–738 (2000). https://doi.org/10.1007/s11745-000-0579-x
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DOI: https://doi.org/10.1007/s11745-000-0579-x