Abstract
Candidate division JS1-and Chloroflexi-related bacteria are ubiquitous in various deep marine sediments worldwide, yet almost nothing is known about their abundance and diversity in cold seep sediments. Here, we investigated the abundance and diversity of JS1- and Chloroflexi-related bacteria in a cold seep marine sediment core collected from the northern South China Sea (SCS) with the employment of quantitative polymerase chain reaction (qPCR) and 16S rRNA gene phylogenetic analyses. The qPCR results showed that 16S rRNA gene copies per gram of sediments for the total bacteria and JS1- and Chloroflexi-related bacteria were at magnitudes of 108 and 106, respectively. The relative abundance of JS1- and Chloroflexi-related 16S rRNA genes to that of total bacteria was 0.07%–8.78% throughout the core. Phylogenetic analyses showed that the JS-1 related clone sequences were dominant throughout the core. Our study provided insights into abundance and diversity of JS1- and Chloroflexi-related bacteria in the northern SCS cold seep sediments.
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Yong Zhang is a Ph.D. candidate at School of Ocean Sciences, China University of Geosciences (Beijing). He obtained his B.S. degree at Shandong University (Weihai) in microbiology and his M.S. degree at China University of Geosciences (Beijing) in marine geology, respectively. His Ph.D. major is geomicrobiology. His research interests focus on extremophiles in microbial diversity and microbial biogeochemical processes in gas hydrate-bearing sediments. He has published 3 scientific papers on microbial diversity in sediments in the South China Sea.
Xin Su obtained her B.S. and M.S. from China University of Geosciences in 1982 and 1985, respectively and Ph.D. from Christian-Albrechts-Universität zu Kiel (Kiel, Germany) in 1995 Now she is a Professor in School of Ocean Sciences at China University of Geosciences (Beijing). Her research interests focus on micropaleontology (e.g., stratigraphy, palaeoecology) and marine geology (e.g., gas hydrate, mid-ocean ridge hydrothermal vent, and paleooceanography). She has joined multiple international and national scientific oceanic expeditions. Dr. Su has authored more than 60 peer-reviewed journal articles in her research area.
Hongchen Jiang obtained his B.S. degree from Beijing Capital Normal University in 2000, M.S. degree from China University of Geosciences (Beijing) in 2003, and Ph.D. degree from Miami University (Oxford, Ohio) in 2007, respectively. He worked as a Postdoc Research Associate in the University of Oklahoma in 2008. Now he is a Professor in the State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences (Wuhan). His expertise is in the area of geomicrobiology in extreme environments (e.g., saline lakes, hot springs, permafrost, and deep subsurface). Much of his work focuses on microbial diversity and ecological function under extreme environmental conditions (e.g., high- and lowtemperature, high salinity), and microbial response to environmental change. Dr. Jiang has published more than 40 peer-reviewed research articles in his research area.
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Zhang, Y., Su, X., Chen, F. et al. Abundance and diversity of candidate division JS1- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea. Front. Earth Sci. 6, 373–382 (2012). https://doi.org/10.1007/s11707-012-0324-0
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DOI: https://doi.org/10.1007/s11707-012-0324-0