Abstract
The gelatin–glutaraldehyde (gelatin–GA) nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity. The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolytic resistance, mechanical property and biocompatibility of nanofiber scaffolds were tested and characterized. The gelatin–GA nanofiber has nice uniform diameter and more than 80% porosity. The hydrolytic resistance and mechanical property of the gelatin–GA nanofiber scaffolds are greatly improved compared with that of gelatin nanofibers. The contact angle, moisture absorption, hydrolysis resistance, thermal resistance and mechanical property of gelatin–GA nanofiber scaffolds could be adjustable by varying the gelatin solution concentration and GA content. The gelatin–GA nanofibers had excellent properties, which are expected to be an ideal scaffold for biomedical and tissue engineering applications.
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Matthews J A, Wnek G E, Simpson D G, et al. Electrospinning of collagen nanofibers. Biomacromolecules, 2002, 3(2): 232–238
Ma Z, Kotaki M, Inai R, et al. Potential of nanofiber matrix as tissue-engineering scaffolds. Tissue Engineering, 2005, 11(1–2): 101–109
Sisson K, Zhang C, Farach-Carson M C, et al. Fiber diameters control osteoblastic cell migration and differentiation in electrospun gelatin. Journal of Biomedical Materials Research Part A, 2010, 94A(4): 1312–1320
Zhang S, Huang Y, Yang X, et al. Gelatin nanofibrous membrane fabricated by electrospinning of aqueous gelatin solution for guided tissue regeneration. Journal of Biomedical Materials Research Part A, 2009, 90A(3): 671–679
Choi M O, Kim Y J. Fabrication of gelatin/calcium phosphate composite nanofibrous membranes by biomimetic mineralization. International Journal of Biological Macromolecules, 2012, 50(5): 1188–1194
Baiguera S, Del Gaudio C, Lucatelli E, et al. Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. Biomaterials, 2014, 35(4): 1205–1214
Dhandayuthapani B, Krishnan U M, Sethuraman S. Fabrication and characterization of chitosan–gelatin blend nanofibers for skin tissue engineering. Journal of Biomedical Materials Research Part B, 2010, 94B(1): 264–272
Meng Z X, Xu X X, Zheng W, et al. Preparation and characterization of electrospun PLGA/gelatin nanofibers as a potential drug delivery system. Colloids and Surfaces B: Biointerfaces, 2011, 84(1): 97–102
Huang C H, Chi C Y, Chen Y S, et al. Evaluation of proanthocyanidin-crosslinked electrospun gelatin nanofibers for drug delivering system. Materials Science and Engineering C, 2012, 32(8): 2476–2483
Chong E J, Phan T T, Lim I J, et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomaterialia, 2007, 3(3): 321–330
Sisson K, Zhang C, Farach-Carson MC, et al. Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability. Biomacromolecules, 2009, 10(7): 1675–1680
Gomes S R, Rodrigues G, Martins G G, et al. In vitro evaluation of crosslinked electrospun fish gelatin scaffolds. Materials Science and Engineering C, 2013, 33(3): 1219–1227
Panzavolta S, Gioffrè M, Focarete M L, et al. Electrospun gelatin nanofibers: optimization of genipin cross-linking to preserve fiber morphology after exposure to water. Acta Biomaterialia, 2011, 7 (4): 1702–1709
Juthamas R, Ratthapol R, Hathairat J, et al. Influences of physical and chemical crosslinking techniques on electrospun type A and B gelatin fiber mats. International Journal of Biological Macromolecules, 2010, 47(4): 431–438
Chen Z, Wang L, Jiang H. The effect of procyanidine crosslinking on the properties of the electrospun gelatin membranes. Biofabrication, 2012, 4(3): 035007
Reddy N, Reddy R, Jiang Q. Crosslinking biopolymers for biomedical applications. Trends in Biotechnology, 2015, 33(6): 362–369
Jalaja K, Kumar P R A, Dey T, et al. Modified dextran crosslinked electrospun gelatin nanofibres for biomedical applications. Carbohydrate Polymers, 2014, 114: 467–475
Jalaja K, James N R. Electrospun gelatin nanofibers: a facile crosslinking approach using oxidized sucrose. International Journal of Biological Macromolecules, 2015, 73: 270–278
Tang C, Saquing C D, Harding J R, et al. In situ cross-linking of electrospun poly(vinyl alcohol) nanofibers. Macromolecules, 2010, 43(2): 630–637
Cao M, Chen Z, Tu K, et al. Studies on one-step electrospinning for preparing crosslinked gelatin fibers. Acta Polymerica Sinica, 2009, 9(11): 1157–1161 (in Chinese)
Erencia M, Cano F, Tornero J A, et al. Electrospinning of gelatin fibers using solutions with low acetic acid concentration: Effect of solvent composition on both diameter of electrospun fibers and cytotoxicity. Journal of Applied Polymer Science, 2015, 132(25): 1–11
Zhu X, Cui W, Li X, et al. Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules, 2008, 9(7): 1795–1801
Mei L, Hu D, Ma J, et al. Preparation, characterization and evaluation of chitosan macroporous for potential application in skin tissue engineering. International Journal of Biological Macromolecules, 2012, 51(5): 992–997
Hoque M S, Benjakul S, Prodpran T. Effect of heat treatment of film-forming solution on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin. Journal of Food Engineering, 2010, 96(1): 66–73
Chen X, Li W, Shao Z, et al. Separation of alcohol-water mixture by pervaporation through a novel natural polymer blend membrane-chitosan/silk fibroin blend membrane. Journal of Applied Polymer Science, 1999, 73(6): 975–980
Okuyama K. Revisiting the molecular structure of collagen. Connective Tissue Research, 2008, 49(5): 299–310
Chen Z, Wang L, Jiang H. The effect of procyanidine crosslinking on the properties of the electrospun gelatin membranes. Biofabrication, 2012, 4(3): 035007
Amadori S, Torricelli P, Rubini K, et al. Effect of sterilization and crosslinking on gelatin films. Journal of Materials Science: Materials in Medicine, 2015, 26(2): 69–70
Bigi A, Panzavolta S, Rubini K. Relationship between triple-helix content and mechanical properties of gelatin films. Biomaterials, 2004, 25(25): 5675–5680
Ki C S, Baek D H, Gang K D, et al. Characterization of gelatin nanofiber prepared from gelatin–formic acid solution. Polymer, 2005, 46(14): 5094–5102
Song J H, Kim H E, Kim H W. Production of electrospun gelatin nanofiber by water-based co-solvent approach. Journal of Materials Science: Materials in Medicine, 2008, 19(1): 95–102
Ren L, Wang J, Yang F Y, et al. Fabrication of gelatin–siloxane fibrous mats via sol–gel and electrospinning procedure and its application for bone tissue engineering. Materials Science and Engineering C, 2010, 30(3): 437–444
Usha R, Ramasami T. Effect of crosslinking agents (basic chromium sulfate and formaldehyde) on the thermal and thermomechanical stability of rat tail tendon collagen fibre. Thermochimica Acta, 2000, 356(1–2): 59–66
de Carvalho R A, Grosso C R F. Characterization of gelatin based films modified with transglutaminase, glyoxal and formaldehyde. Food Hydrocolloids, 2004, 18(5): 717–726
Zhang Y Z, Venugopal J, Huang Z M, et al. Crosslinking of the electrospun gelatin nanofibers. Polymer, 2006, 47(8): 2911–2917
Bigi A, Cojazzi G, Panzavolta S, et al. Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking. Biomaterials, 2001, 22(8): 763–768
Winter G D. Some factors affecting skin and wound healing. Journal of Tissue Viability, 2006, 16(2): 20–23
Winter G D, Scales J T. Effect of air drying and dressings on the surface of a wound. Nature, 1963, 197(4862): 91–92
Metzger S. Clinical and financial advantages of moist wound management. Home Healthcare Nurse, 2004, 22(9): 586–590
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Zhan, J., Morsi, Y., Ei-Hamshary, H. et al. In vitro evaluation of electrospun gelatin–glutaraldehyde nanofibers. Front. Mater. Sci. 10, 90–100 (2016). https://doi.org/10.1007/s11706-016-0329-9
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DOI: https://doi.org/10.1007/s11706-016-0329-9