Abstract
Polyetheretherketone (PEEK) is regarded as one of the most potential candidates of biomaterials in spinal implant applications. However, as a bioinert material, PEEK plays a limited role in osteoconduction and osseointegration. In this study, recombinant human bone morphogenetic protein-2 (rhBMP-2) was immobilized onto the surface of collagen-coated PEEK in order to prepare a multi-functional material. After adsorbed onto the PEEK surface by hydrophobic interaction, collagen was cross-linked with N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). EDC/NHS system also contributed to the immobilization of rhBMP-2. Water contact angle tests, XPS and SEM clearly demonstrated the surface changes. ELISA tests quantified the amount of rhBMP-2 immobilized and the release over a period of 30 d. In vitro evaluation proved that the osteogenesis differentiation rate was higher when cells were cultured on modified PEEK discs than on regular ones. In vivo tests were conducted and positive changes of major parameters were presented. This report demonstrates that the rhBMP-2 immobilized method for PEEK modification increase bioactivity in vitro and in vivo, suggesting its practicability in orthopedic and spinal clinical applications.
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Bishop S M. The mechanical performance and impact behaviour of carbon-fibre reinforced PEEK. Composite Structures, 1985, 3(3): 295–318
Fujihara K, Huang Z M, Ramakrishna S, et al. Feasibility of knitted carbon/PEEK composites for orthopedic bone plates. Biomaterials, 2004, 25(17): 3877–3885
Searle O B, Pfeiffer R H. Victrex® poly(ethersulfone) (PES) and Victrex® poly(etheretherketone) (PEEK). Polymer Engineering and Science, 1985, 25(8): 474–476
Maharaj G R, Jamison R D. Intraoperative impact: characterization and laboratory simulation on composite hip prostheses. ASTM Special Technical Publication, 1993, 1178: 98
Du Y W, Zhang L N, Hou Z T, et al. Physical modification of polyetheretherketone for orthopedic implants. Frontiers of Materials Science, 2014, 8(4): 313–324
Kurtz S M, Devine J N. PEEK biomaterials in trauma, orthopedic, and spinal implants. Biomaterials, 2007, 28(32): 4845–4869
Lee J H, Jang H L, Lee KM, et al. In vitro and in vivo evaluation of the bioactivity of hydroxyapatite-coated polyetheretherketone biocomposites created by cold spray technology. Acta Biomaterialia, 2013, 9(4): 6177–6187
Ratner B D, Hoffman A S, Schoen F J, et al. Biomaterials Science: An Introduction to Materials in Medicine. 2nd ed. Elsevier, 2004
Jacquemart I, Pamuła E, De Cupere V M, et al. Nanostructured collagen layers obtained by adsorption and drying. Journal of Colloid and Interface Science, 2004, 278(1): 63–70
He J, Su Y, Huang T, et al. Effects of material and surface functional group on collagen self-assembly and subsequent cell adhesion behaviors. Colloids and Surfaces B: Biointerfaces, 2014, 116: 303–308
Bronk J K, Russell B H, Rivera J J, et al. A multifunctional streptococcal collagen-mimetic protein coating prevents bacterial adhesion and promotes osteoid formation on titanium. Acta Biomaterialia, 2014, 10(7): 3354–3362
de Assis A F, Beloti M M, Crippa G E, et al. Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface. Clinical Oral Implants Research, 2009, 20(3): 240–246
Marín-Pareja N, Salvagni E, Guillem-Marti J, et al. Collagen-functionalised titanium surfaces for biological sealing of dental implants: effect of immobilisation process on fibroblasts response. Colloids and Surfaces B: Biointerfaces, 2014, 122: 601–610
Marchand-Brynaert J, Pantano G, Noiset O. Surface fluorination of PEEK film by selective wet-chemistry. Polymer, 1997, 38(6): 1387–1394
Ying P, Jin G, Tao Z. Competitive adsorption of collagen and bovine serum albumin-effect of the surface wettability. Colloids and Surfaces B: Biointerfaces, 2004, 33(3): 259–263
Hanagata N, Takemura T, Monkawa A, et al. Pre-adsorbed type-I collagen structure-dependent changes in osteoblastic phenotype. Biochemical and Biophysical Research Communications, 2006, 344(4): 1234–1240
De Cupere V M, Rouxhet P G. Collagen films adsorbed on native and oxidized poly (ethylene terephtalate): morphology after drying. Surface Science, 2001, 491(3): 395–404
Woodcock S E, Johnson W C, Chen Z. Collagen adsorption and structure on polymer surfaces observed by atomic force microscopy. Journal of Colloid and Interface Science, 2005, 292(1): 99–107
Olde Damink L H H, Dijkstra P J, van Luyn M J A, et al. In vitro degradation of dermal sheep collagen cross-linked using a watersoluble carbodiimide. Biomaterials, 1996, 17(7): 679–684
Olde Damink L H H, Dijkstra P J, van Luyn M J A, et al. Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. Biomaterials, 1996, 17(8): 765–773
Wissink M J B, Beernink R, Pieper J S, et al. Immobilization of heparin to EDC/NHS-crosslinked collagen. Characterization and in vitro evaluation. Biomaterials, 2001, 22(2): 151–163
Wan M, Cao X. BMP signaling in skeletal development. Biochemical and Biophysical Research Communications, 2005, 328(3): 651–657
Canalis E, Economides A N, Gazzerro E. Bone morphogenetic proteins, their antagonists, and the skeleton. Endocrine Reviews, 2003, 24(2): 218–235
Kübler N R, Reuther J F, Faller G, et al. Inductive properties of recombinant human BMP-2 produced in a bacterial expression system. International Journal of Oral and Maxillofacial Surgery, 1998, 27(4): 305–309
Zhao B, Katagiri T, Toyoda H, et al. Heparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-2. The Journal of Biological Chemistry, 2006, 281(32): 23246–23253
Kim S E, Song S H, Yun Y P, et al. The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function. Biomaterials, 2011, 32(2): 366–373
Shen H, Hu X, Yang F, et al. The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds. Biomaterials, 2009, 30(18): 3150–3157
Visser R, Arrabal P M, Becerra J, et al. The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo. Biomaterials, 2009, 30(11): 2032–2037
Noiset O, Schneider Y J, Marchand-Brynaert J. Adhesion and growth of CaCo2 cells on surface-modified PEEK substrata. Journal of Biomaterials Science: Polymer Edition, 2000, 11(7): 767–786
Noiset O, Schneider Y J, Marchand-Brynaert J. Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces. Journal of Biomaterials Science: Polymer Edition, 1999, 10(6): 657–677
Matrab T, Chehimi M M, Boudou J P, et al. Surface functionalization of ultrananocrystalline diamond using atom transfer radical polymerization (ATRP) initiated by electro-grafted aryldiazonium salts. Diamond and Related Materials, 2006, 15(4): 639–644
Kyomoto M, Moro T, Takatori Y, et al. Self-initiated surface grafting with poly(2-methacryloyloxyethyl phosphorylcholine) on poly(ether-ether-ketone). Biomaterials, 2010, 31(6): 1017–1024
Puleo D A, Bizios R, eds. Biological Interactions on Materials Surfaces: Understanding and Controlling Protein, Cell, and Tissue Responses. New York: Springer US, 2009
Kadler K E, Holmes D F, Trotter J A, et al. Collagen fibril formation. The Biochemical Journal, 1996, 316(Pt 1): 1–11
Zeeman R, Dijkstra P J, van Wachem P B, et al. Successive epoxy and carbodiimide cross-linking of dermal sheep collagen. Biomaterials, 1999, 20(10): 921–931
Alghamdi H S, Bosco R, van den Beucken J J J P, et al. Osteogenicity of titanium implants coated with calcium phosphate or collagen type-I in osteoporotic rats. Biomaterials, 2013, 34(15): 3747–3757
Ben-David D, Srouji S, Shapira-Schweitzer K, et al. Low dose BMP-2 treatment for bone repair using a PEGylated fibrinogen hydrogel matrix. Biomaterials, 2013, 34(12): 2902–2910
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Du, YW., Zhang, LN., Ye, X. et al. In vitro and in vivo evaluation of bone morphogenetic protein-2 (BMP-2) immobilized collagen-coated polyetheretherketone (PEEK). Front. Mater. Sci. 9, 38–50 (2015). https://doi.org/10.1007/s11706-015-0276-x
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DOI: https://doi.org/10.1007/s11706-015-0276-x