Abstract
This paper was the first report on pathogenic bacteria isolated from golden pompano (Trachinotus blochii) with the antibiogram, as well as the heavy metal resistance pattern. Golden pompano becomes popular among Malaysian fish farmers due to its high value and demand from local and oversea markets. However, the baseline information on antibiogram of pathogenic bacteria associated with golden pompano is not well established. Therefore, the information from this study may be useful to fish farmers in selecting appropriate antibiotic for treatment and prophylactive purpose in golden pompano culture. Isolation of bacterial isolates was carried out using 5% of Horse Blood agar, Tryptic Soy Agar (TSA), Mac Conkey, Thiosulphate Citrate Bile Salt (TCBS), Eosin Methylene Blue (EMB), Glutamate Starch Pseudomonas (GSP), Xylose Lysine Deoxycholate (XLD), and Baird Parker media. The bacterial isolates were then identified using conventional biochemical tests and confirmed by commercial bacterial identification kit. Antibiotic susceptibility test of bacterial isolates against 21 antibiotics (oxolinic acid 2 μg, ampicillin 10 μg, erythromycin 15 μg, furazolidone 15 μg, lincomycin 15 μg, oleandomycin 15 μg, amoxicillin 25 μg, colistin sulphate 25 μg, sulpha-methoxazole 25 μg, chloramphenicol 30 μg, doxycycline 30 μg, florfenicol 30 μg, flumequine 30 μg, kanamycin 30 μg, nalidixic acid 30 μg, novobiocin 30 μg, oxytetracycline 30 μg, tetracycline 30 μg, nitrofurantoin 50 μg, fosfomycin 50 μg, and spiramycin 100 μg) was determined using disk diffusion method, whereas the heavy metal resistance pattern (mercury Hg2+, cadmium Cd2+, chromium Cr6 +, and copper Cu2+) of the bacterial isolates was characterized using two-fold agar dilution method. Five bacterial species were successfully found from 50 diseased golden pompano. They were Streptococcus spp. (n = 12), Escherichia coli (n = 30), Salmonella spp. (n = 20), Pseudomonas spp. (n = 36), and Vibrio spp. (n = 50). More than 80% of the bacterial isolates were found sensitive to 11 out of 21 antibiotics (tetracycline, nitrofurantoin, florfenicol, chloramphenicol, oxytetracycline, nalidixic acid, doxycycline, furazolidone, flumequine, fosfomycin, and oxolinic acid. However, all bacterial isolates were found resistant to all tested heavy metals except for copper and cadmium. The multiple antibiotic resistance (MAR) index of the present study indicated that the fish samples were under high risk exposure to the tested antibiotics. Florfenicol is suggested to be used as antimicrobial agent for golden pompano culture since all bacterial isolates were sensitive to it.
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References
Allen D A, Austin B, Colwell R R (1977). Antibiotic resistance patternsof metal-tolerant bacteria isolated from an estuary. Antimicrobial Agents and Chemotherapy, 12(4): 545–547
Bauer A W, Kirby W M, Sherris J C, Turck M (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4): 493–496
CLSI (Clinical and Laboratory Standards Institute) (2006). Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. CLSI document M100-S16 Vol. 26 No.3. USA: Clinical and Laboratory Standards Institute.
Garza-Gil M D, Varela-Lafuente M, Caballero-Miguez G (2009). Price and production trends in the marine fish aquaculture in Spain. Aquaculture Research, 40: 274–281
Gaunt P S, McGinnis A L, Santucci T D, Cao J, Waeger P, Endrid R G (2006). Field efficacy of florfenicol for control of mortality in channel catfish, Ictalurus punctatus (Rafinesque), Caused by infection with Edwardsiella ictaluri. Journal of the World Aquaculture Society, 37(1): 1–11
Holt G, Krieg N R, Sneath P H A, Staley J T, Williams S T (1994). Bergey’s Manual of Determinative Bacteriology. 9th edition. USA: Lippincott Williams and Wilkins
Inglis V, Richards R H, Varma K J, Sutherland I, Brokken E S (1991). Florfenicol in Atlantic salmon, Salmo salar L., parr: tolerance and assessment of efficacy against furunculosis. Journal of Fish Disease, 14: 343–351
Lazo J P, Davis D A, Arnold C R (1998). The effects of dietary protein level on growth, feed efficiency and survival of juvenile Florida pompano (Trachinotus carolinus). Aquaculture, 168: 225–232
Matsuyama T, Kamashi T, Ooseko N, Kurohara K, Iida T (2005). Pathogenic of motile and non-motile Edwardsiella tarda to some marine fish. Fish Pathology, 40(3): 133–135
Miranda C D, Castillo G (1998). Resistance to antibiotic and heavy metals of motile aeromonads from Chilean freshwater. The Science of the Total Environment, 224: 167–176
Miranda C D, Zemelman R (2001). Antibiotic resistant bacteria in fish from the Concepcion Bay, Chile. Marine Pollution Bulletin, 42(11): 1096–1102
Nordmo R, Riseth J M H, Varma K J, Sutherland I H, Brokken E S (1998). Evaluation of florfenicol in Atlantic salmon, Salmo salar L.: efficacy against furunculosis due to Aeromonas salmonicida and cold water vibriosis due to Vibrio salmonicida. Journal of Fish Diseases, 21: 289–297
Sampaio L A, Tesser M B, Burkert D (2003). Acute salinity tolerance of Juvenile pompano Trachinotus marginatus (Teleostei, Carangidae) under laboratory conditions. Ciência Rural, 33(4): 757–761
Costa L D F, Miranda-Filho K C, Severo M P, Sampaio L A (2008). Tolerance of juvenile pompano Trachinotus marginatus to acute ammonia and nitrite exposure at different salinity levels. Aquaculture, 285: 270–272
Samuelsen O B, Bergh Ø (2004). Efficacy of orally administered florfenicol and oxolinic acid for the treatment of vibriosis in cod (Gadus morhua). Aquaculture, 235: 27–35
Samuelsen O B, Hjeltnes B, Glette J (1998). Efficacy of orally administered florfenicol in the treatment of furunculosis in Atlantic salmon. Journal of Aquatic Animal Health, 10: 56–61
Sarter S, Nguyen H N K, Hung L T, Lazard J, Montet D (2007). Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish. Food Control, 18: 1391–1396
Toranzo A E, Magarinos B, Romalde J L (2005). A review of the main bacterial fish diseases in mariculture systems. Aquaculture, 246: 37–61
Yanong R P E, Curtis EW, Simmons R, Bhattaram V A, Gopalakrishnan M, Ketabi N, Nagaraja N V, Derendorf H (2005). Pharmacokinetic studies of florfenicol in koi carp and Threespot gourami Trichogaster trichopterus after oral and intramuscular treatment. Journal of Aquatic Animal Health, 17: 129–137
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Lee, S.W., Najiah, M. & Wendy, W. Bacteria associated with golden pompano (Trachinotus blochii) broodstock from commercial hatchery in Malaysia with emphasis on their antibiotic and heavy metal resistances. Front. Agric. China 4, 251–256 (2010). https://doi.org/10.1007/s11703-010-0106-y
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DOI: https://doi.org/10.1007/s11703-010-0106-y