Abstract
Objective: To study the effect of active compound 6F and A from Pteris semipinnata L.(PsL) on the activities of DNA topoisomerase (TOPO) I and II, activities of cytosolic and membrane TPK, and expression of oncogene c-myc in lung adenocarcinoma cells. Methods: The effect of compound 6F and A on activities of cytosolic and membrane TPK was measured by scintillation counting; the effect of compound A on expression of oncogene c-myc was determined by flow cytometry indirect fluorimetry. Results: compound 6F and A could inhibit the activities of TOPO I, and they strongly inhibited the TOPO II in 0.01 mg/L and 10.0 mg/L respectively. Compound A slightly inhibited the activities of membrane TPK, but not the cytosolic one. Compound A could inhibit the expression of oncogene c-myc. Conclusion: Topoisomerases are target of compound 6F and A. Compound A could slightly inhibit the activities of TPK, and showed an inhibitory effect on the expression of oncogene c-myc.
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Foundation item: This work was supported by the National Natural Science Foundation of China (No. 39870900) and the key project grant from Guangdong Province Science and Technology Commission (No. 9622007-002).
Biography: LI Jin-hua, (1969–), lecturer, master of medicine, Guangdong Medical College, majors in biochemistry.
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Li, Jh., Liang, Nc., Mo, Le. et al. Effect of active compounds isolated from Pteris semipinnata L on DNA topoisomerases and tyrosine protein kinase and expression of C-MYC in lung adenocarcinoma cells. Chin. J. Cancer Res. 13, 105–109 (2001). https://doi.org/10.1007/s11670-001-0025-9
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DOI: https://doi.org/10.1007/s11670-001-0025-9