Summary
Minimal bactericidal concentrations (MBCs) were determined for 16 bacterial strains isolated from strawberry runners. Bacteria were treated with single antibiotics: Timentin, streptomycin sulfate, gentamicin, and dihydrostreptomycin; and with combinations of two or three antibiotics: Timentin, streptomycin sulfate, and gentamicin. Combinations of the three antibiotics (12) were effective with all bacteria tested and were then used to treat contaminated plantlets. Fragaria × ananassa Duch. cv. Jucunda inoculated with Xanthomonas campestris pv. vesicatoria or Pseudomonas corrugata were grown for 1 wk, then treated with combinations of Timentin, streptomycin, and gentamicin. Antibiotic treatments were 100% effective in eliminating P. corrugata from ‘Jucunda,’ but only 23% of the plants inoculated with X. campestris pv. vesicatoria were freed of the bacteria. Phytotoxicity was observed only at high antibiotic concentrations. Detection of bacteria from treated plants was most effective after one subculture, as antibiotics continued to inhibit bacterial growth on detection medium immediately after treatment.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Barrett, C.; Cassells, A. C. An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium × domesticum cv. ‘Grand Slam’ Plant Cell Tissue Organ Cult. 36:169–175; 1994.
Bastiaens, L. Endogenous bacteria in plants and their implications in tissue culture—a review. Med. Fac. Landbouww. Rijksuniv. Gent. 48:1–11; 1983.
Boxus, P.; Terzi, J. M. Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Hort. 212:91–93; 1987.
Buckley, P. M.; DeWilde, T. N.; Reed, B. M. Characterization and identification of bacteria isolated from micropropagated mint plants. In Vitro Cell. Dev. Biol. 31P:58–64; 1995.
Cassells, A. C. Problems in tissue culture: culture contamination. In: Debergh, P. C.; Zimmerman, R. H., ed. Micropropagation technology and application. Dordrecht, Netherlands: Kluwer Academic Publishers; 1991:31–44.
Falkiner, F. R. Strategy for the selection of antibiotics for use against common bacterial pathogens and endophytes of plants. Acta Hort. 225:53–56; 1988.
Falkiner, F. R. The criteria for choosing an antibiotic for control of bacteria in plant tissue culture. Newsletter, International Association for Plant Tissue Culture 60:13–23; 1990.
Kneifel, W.; Leonhardt, W. Testing of different antibiotics against gram positive and gram negative bacteria isolated from plant tissue cultures. Plant Cell Tissue Organ Cult. 29:139–144; 1992.
Leifert, C.; Camotta, H.; Wright, S. M., et al. Elimination of Lactobacillus plantarum, Corynebacterium spp., Staphylococcus saprophyticus and Pseudomonas paucimobilis from micropropagated Hemerocallis, Choisya and Delphinium cultures using antibiotics. J. Appl. Bacteriol. 71:307–330; 1991.
Leifert, C.; Waites, W. M. Bacterial growth in plant tissue culture media. J. Appl. Bacteriol. 72:460–466; 1992.
Leifert, C.; Waites, W. M. Dealing with microbial contaminants in plant tissue and cell culture: hazard analysis and critical control points. In: Lumsden, P. J.; Nicholar, J. R.; Davies, W. J., ed. Physiology, growth and development of plants in culture. Dordrecht, Netherlands: Kluwer Academic Publishers; 1994:363–378.
Mathias, P. J.; Alderson, P. G.; Leakey, R. R. B. Bacterial contamination in tropical hardwood cultures. Acta Hort. 212:43–48; 1987.
Reed, B. M.; Tanprasert, P. Detection and control of bacterial contaminants of plants tissue cultures. A review of recent literature. Plant Tissue Culture Biotech. 1:137–142; 1995.
Tanprasert, P. Detection, identification, and antibiotic treatment of bacterial contaminants from micropropagated strawberries. M.S. Thesis, Oregon State University, 1996.
Viss, P. R.; Brooks, E. M.; Driver, J. A. A simplified method for the control of bacterial contamination in woody plant tissue culture. In Vitro Cell. Dev. Biol. 27P:42; 1991.
Author information
Authors and Affiliations
Additional information
Parts of this manuscript are included in a summary of a poster presented at the Second International Symposium on Bacterial and Bacteria-Like Contaminants of Plant Tissue Cultures, Cork, Ireland, September 1996.
Rights and permissions
About this article
Cite this article
Tanprasert, P., Reed, B.M. Determination of minimal bactericidal and effective antibiotic treatment concentrations for bacterial contaminants from micropropagated strawberries. In Vitro Cell.Dev.Biol.-Plant 33, 227–230 (1997). https://doi.org/10.1007/s11627-997-0027-5
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-997-0027-5