Summary
Desiccation tolerance can be induced by culturing somatic embryos of loblolly pine (Pinus taeda L.) on medium supplemented with 50 μM abscisic acid (ABA) and/or 8.5% polyethylene glycol (PEG6000). Scanning electron microscopy of desiccated somatic embryos showed that the size and external morphology of the desiccation-tolerant somatic embryos recovered to the pre-desiccation state within 24–36 h, whereas the non-desiccation-tolerant somatic embryos did not recover and remained shriveled, after rehydration. Peroxidase activity of desiccated somatic embryos increased sharply after 1 d of desiccation treatment at 87% relative humidity (RH), and desiccation-tolerant somatic embryos had higher peroxidase activity compared to sensitive somatic embryos. Higher peroxidase activity of desiccation-tolerant somatic embryos may have allowed them to catalyze the reduction of H2O2 produced by drought stress, and protected them from oxidative damage.
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Tang, W. Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos. In Vitro Cell.Dev.Biol.-Plant 36, 488–491 (2000). https://doi.org/10.1007/s11627-000-0087-2
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DOI: https://doi.org/10.1007/s11627-000-0087-2