Summary
Root apices from in vitro cultured garlic (Allium sativum) cloves of cvs. ABEN and GT96-1 were used as axenic explants for organogenic callus production and plant regeneration experiments. Explants cultured in media based on those of Chu and co-workers (N6) or Murashige and Skoog (MS) could induce organogenic callus after 8 wk culture in darkness. Both media were supplemented with 2,4-dichlorophenoxyacetic acid (2.2–4.5 μM), alone or combined with 6-furfurylaminopurine (kinetin, 2.3–4.6 μM). Shoots started to grow 3 wk after culturing in the presence of light and the addition to culture media of 4.4 μM N6-benzyladenine. Plants capable of producing microbulbs regenerated 6 wk later. Up to 170 plants g−1 FW callus were obtained when culturing was initiated in MS medium supplemented with 4.6 μM kinetin and 4.5 μM 2,4-dichlorophenoxyacetic acid.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abo El-Nil, M. M. Organogenesis and embryogenesis in callus cultures of garlic (Allium sativum L.). Plant Sci. Lett. 9:259–264; 1977.
Al-Zahim, M. A.; Ford-Lloyd, B. V.; Newbury, H. J. Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis. Plant Cell Rep. 18:473–477; 1999.
Armstrong, C. L.; Green, C. E. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164:207–214; 1985.
Ayabe, M.; Sumi, S. Establishment of a novel tissue culture method, stemdisc culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Rep. 17:773–779; 1998.
Barandiaran, X.; Martín, N.; Rodríguez-Conde, M. F.; Di Pietro, A.; Martín, J. An efficient method for callus culture and shoot regeneration of garlic (Allium sativum L.). HortSci 34:348–349; 1999a.
Barandiaran, X.; Martín, N.; Rodríguez-Conde, M. F.; Di Pietro, A.; Martín, J. Genetic variability in callus formation and regeneration of garlic (Allium sativum L.). Plant Cell Rep. 18:434–437; 1999b.
Bhojwani, S. S.; Cohen, D.; Fry, P. R. Production of virus-free garlic and field performance of micropropagated plants. Scientia Hortic. 18:39–43; 1982.
Chu, C. C.; Wang, C. C.; Sun, C. S.; Hsu, C.; Yin, K. C.; Chu, C. Y.; Bi F. Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sinica 18:659–668; 1975.
Davies, J. L. M. Chemical control of Allium white rot: a review. In: Entwistle, A. R.; Melero-Vara, J. M., eds. Proceedings of the Fifth International Workshop of Allium white rot. Cordoba, Spain: Instituto de Agricultura Sostenible; 1994:7–8.
Dustan, D. I.; Short, K. C. Shoot production from onion callus tissue culture. Scientia Hortic. 9:99–110; 1978.
Eriksson, T. Studies on the growth requirements and growth measurements of cell cultures of Haplopappus gracilis. Physiol. Plant. 18:976–993; 1965.
Haque, M. S.; Wada, T.; Hattori, K. High frequency shoot regeneration and plantlet formation from root tip of garlic. Plant Cell Tiss. Organ Cult. 50:83–89; 1997.
Havel, L.; Novák, F. J. Regulation of somatic embryogenesis and organogenesis in Allium carinatum L. Plant Physiol. 132:373–377; 1988.
Havránek, P.; Novák, F. J. The bud formation in callus cultures of Allium sativum L. Z. Pflanzenphysiol. 68:308–318; 1973.
Kehr, A. E.; Schaeffer, G. W. Tissue culture and differentiation of garlic. HortSci. 11:422–423; 1976.
Laborde, C. J. A. Coexistence of garlic white rot with commercial production in Central Mexico. In: Entwistle, A. R., ed. Proceedings of the Third International Workshop on Allium white rot, Wellesbourne, UK, 1986. Leamington Spa: Printing Associates; 1987:24–40.
Ma, Y.; Wang, H. L.; Zhang, C. J.; Kang, Y. Q. High rate of virus free plantlet regeneration via garlic scape tip culture. Plant Cell Rep. 14:65–68; 1994.
Mohamed-Yassen, Y.; Splittstoesser, W. E.; Litz, R. E. In vitro shoot proliferation and production of sets from garlic and shallot. Plant Cell Tiss. Organ Cult. 36:243–247; 1994.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Myers, J. M.; Simon, P. W. Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets. Plant Cell Rep. 17:726–730; 1998.
Nagakubo, T.; Nagasawa, A.; Ohkawa, H. Micropropagation of garlic through in vitro bulblet formation. Plant Cell Tiss. Organ Cult. 32:175–183; 1993.
Novák, F. J. Allium tissue culture. In: Rabinowitch, H. D.; Brewster, J. L., eds. Onions and allied crops, Vol. 1. Boca Raton, FL: CRC Press; 1990:233–250.
Novák, F. J.; Havel, L.; Dolezél, J. Onion, garlic and leek (Allium species). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, Vol. 2, Crops, I. Berlin: Springer-Verlag; 1986:387–404.
Shahin, E. A.; Kaneko, K. Somatic embryogenesis and plant regeneration from callus cultures of nonbulbing onion. HortSci. 21:294–295; 1986.
Shavemaker, C. M.; Jacobsen, E. Development of cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos. Plant Cell Rep. 14:227–231; 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Robledo-Paz, A., Villalobos-Arámbula, V.M. & Jofre-Garfias, A.E. Efficient plant regeneration of garlic (Allium Sativum L.) by root-tip culture. In Vitro Cell.Dev.Biol.-Plant 36, 416–419 (2000). https://doi.org/10.1007/s11627-000-0075-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-000-0075-6