Summary
This study observed the protective effect of hypercapnic acidosis preconditioning on rabbit heart suffered from ischemia-reperfusion injury. Hypercapnic acidosis was established in animals with mechanical hypoventilation before ischemia-reperfusion. Thirty-two rabbits were randomly divided into 4 groups, with each having 8 aminals in term of the degree of acidification: hypercapnic acidosis group A (group A), hypercapnic acidosis group B (group B), hypercapnic acidosis group C (group C), ischemia and reperfusion group (group IR). Animals in group IR were ventilated normally (tidal volume: 15 mL/kg, breathing rate 35 bpm). The PETCO2 was maintained at the level of 40–50 mmHg for 30 min. Animals in groups A, B, C received low-frequency, low-volume ventilation to achieve hypercarbonic acidosis and the target levels of PETCO2 were 75–85,65–75, 55–65 mmHg, respectively, with levels being maintained for 5 min. The animals then were ventilated normally to lower PETCO2 to 40–50 mmHg. The left anterior branch artery of all the animals was ligated for 30 min and reperfused for 180 min. Then the infarct size was calculated. The cardiomyocytes were morphologically observed and ECG and hemodynamics were monitored on continuous basis. Acid-base balance was measured during procedure. Our results showed that the infarct size was (48.5±11.5)% of the risk area in the control group and (42.4±7.9)% in group C (P>0.05). Mean infarct size was significantly smaller in group B (34.5%±9.4%) (P<0.05 vs control group) and group A (31.0%±9.1%) (P<0.01 vs control group). It is concluded that HA-preconditioning can effectively protect the myocardium.
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Murry C E, Jennins R B, Reimer K A. Preconditioning of with ischemia:a delay of lethal cell injury in ischemic myocardium. Circulation, 1986,74(5):1124–1136
Marinovic J, Bosnjak Z J, Stadnicka A. Preconditioning by isoflurane induces lasting sensitization of the cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel by a protein kinase C-delta-mediated mechanism. Anesthesiology, 2005,103(3):540–547
Wu J, Xu H, Yang Z et al. Protons activate homomeric Kir6.2 channels by selective suppression of the long and intermediate closures Direct activation of cloned KATP channels by intracellular acidosis. J Membr Biol, 2002,190(2):105–116
Wang X, Wu J, Li L et al. Hypercapnic acidosis activates KATP channels in vascular smooth muscles. Circ Res, 2003,92(11):1225–1232
Simkhovich B Z, Whittakerp R, Przyklenk K et al. Transient pre-ischemia acidosis protects the isolated rabbit heart subjected to 30 minutes, but not 60 minutes of global ischemia. Basic Res Canliol, 1995,90(5):397–403
Lundmark J A, Trueblood N, Wang L F et al. Repetitive acidosis protects the ischemia heart: implications for mechanisms in preconditioned hearts. J Mol Cell Cardiol, 1999,31(4):907–917
Laffey J G, Honan D, Hopkins N et al. Hypercapnic acidosis attenuates endotoxin-induced acute lung injury. Am J Respir Crit Care Med, 2004,169(1):46–56
Takeshita K, Suzuki Y, Nishio K et al. Hypercapnic acidosis attenuates endotoxin -induced nuclear factor-[kappa]B activation. Am J Respir Cell Mol Biol, 2003,29(1):124–32
Nomura F, Aoki M, Forbess J M et al. Effects of hypercarbic acidotic reperfusion on recovery of myocardial function after cardioplegic ischemia in neonatal lambs. Circulation, 1994,90(5 pt2):II321–II327
Preckel B, Schlack W, Obal D et al. Effect of acidotic blood reperfusion on reperfusion injury after coronary artery occlusion in the dog heart. J Cardiovasc Pharmacol, 1998,31(2):179–186
Morisaki H, Serita R, Innami Y et al. Permissive hypercapnia during thoracic anaesthesia. Acta Anaesthesiol Scand, 1999,43(8):845–849
Feihl F, Perret C. Permissive hypercapnia: How permissive should we be? Am J Res Criti Care Med, 1994,150(6 pt1):1722–1733
Pfeiffer B, Hachenberg T, Wendt M et al. Mechanical ventilation with permissive hypercapnia increases intrapulmonary shunt in septic and nonseptic patients with acute respiratory distress syndrome. Crit Care Med, 2002,30(2):285–289
Gillette M A, Hess D R. Ventilator-induced lung injury and the evolution of lung-protective strategies in acute respiratory distress syndrome. Respir Care, 2001,46(2): 130–148
Mullenheim J, Frassdorf J, Preckel B et al. Ketamine, but not S(+)-ketamine, blocks ischemic preconditioning in rabbit hearts in vivo. Anesthesiology, 2001,94(4):630–636
Schultz J E, Hsu A K, Gross G J. Morphine mimics the cardioprotective effect of ischemic preconditioning via a glibenclamide-sensitive mechanism in the rat heart. Circ Res, 1996,78(6):1100–1104
Baines C P, Pass J M, Ping P. Protein kinases and kinase-modulated effectors in the late phase of ischemic preconditioning. Basic Res Cardiol, 2001,96(3):207–218
Quayle J M, Nelson, M T et al. ATP-sensitive and inwardly rectifying potassium channels in smooth muscle. Physiol Rev, 1997,77(4):1165–1232
Aschcroft F M, Gribble F M. Correlating structure and function in ATP-sensitive K+ channels. Trends Neurosci, 1998,21(7):288–294
Yokoshiki H, Sunagawa M, Seki T et al. ATP-sensitive K+ channels in pancreatic, cardiac, and vascular smooth muscle cells. Am J Physiol, 1998(1 pt1),274:C25–C37
Inagaki N, Gonoi T, Clement J P 4th et al. Reconstitution of IKATP: an inward rectifier subunit plus the sulfonylurea receptor. Science, 1995,270(5239):1166–1170
Xu H, Wu J, Cui N et al. Distinct histidine residues control the acid-induced activation and inhibition of the cloned K(ATP) channel. J Biol Chem, 2001,276(42): 38690–63869
Fan Z, Tokuyama Y, Makielski J C. Modulation of ATP-sensitive K+ channels by internal acidification in insulin-secreting cells. Am J Physiol, 1994,267(4 Pt1): C1036–C1044
Khuri S. Myocardial protection during reoperative valve surgery. In: Englemen RM, Levitsky S, eds. A Textbook of Cardioplegia for Difficult Clinical Problems. NY: Futura Publishing, 1992.221–235
Lange R, Kloner R A, Zierler M et al. Time course of ischemic alterations during normothermic and hypothermic arrest and its reflection by on-line monitoring of tissue pH. J Thorac Cardiovasc Surg, 1983,86(3):418–434
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Heguo LUO, male, born in 1975, Associate Professor
This project was supported by a grant from the Special Foundation for Doctoral Program of the Ministry of Education of People’s Republic of China (No. 20050533022).
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Luo, H., Chang, Y., Cai, H. et al. The effect of hypercapnic acidosis preconditioning on rabbit myocardium. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 706–710 (2008). https://doi.org/10.1007/s11596-008-0621-8
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DOI: https://doi.org/10.1007/s11596-008-0621-8