Abstract
Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S 5 n, revealing that 18 accessions carried the S 5 n allele with a heterozygous genotype (S 5 nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S 5 n allele of O. rufipogon confirmed that this allele is identical with that of 02428, a wide-compatibility variety of domesticated rice. The segregation ratio of the 3 genotypes (S 5 iS 5 i/S 5 jS 5 j:S 5 nS 5 i/j:S 5 nS 5 n) among 15 self-bred progenies of O. rufipogon significantly deviated from the Mendelian expectation (1:2:1) in that the proportion of plants exhibiting S 5 n heterozygous and homozygous genotypes was much lower in the progeny. Sequence analysis of full-length S 5 n alleles indicated that a few base pairs were different between O. rufipogon and O. sativa, and it was presumed that S 5 n may be an ancient allele. The fertility of the embryo sac in plants with an S 5 n heterozygous genotype was low, suggesting the existence of other genes controlling embryo sac fertility in the wild rice.
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References
Lu C G, Zou J S. Research progress in the compatibility of inter-subspecific hybrid rice (Oryza sativa L.) (in Chinese). Jiangsu J Agric Sci, 2000, 16: 50–56
Song X, Qiu S Q. Xu C G, et al. Genetic dissection of embryo sac fertility, pollen fertility, and their contributions to spikelet fertility of intersubspecific hybrids in rice. Theor Appl Genet, 2005, 110: 205–211
Ikehashi H, Araki H. Genetics of F1 sterility in remote crosses of rice. In: IRRI, ed. Rice Genetics. Manila, Philippines: IRRI, 1986. 119–130
Qiu S Q, Liu K D, Jiang J X, et al. Delimitation of the rice wide compatibility gene S 5 n to a 40-kb DNA fragment. Theor Appl Genet, 2005, 111: 1080–1086
Ji Q, Lu J F, Chao Q, et al. Delimiting a rice wide-compatibility gene S 5 n superscript stop to a 50-kb region. Theor Appl Genet, 2005, 111: 1495–1503
Chen J J, Ding J H, Ouyang Y D, et al. A triallelic system of S 5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105: 11436–11441
Yang Y X, Wu J W, Chen Z X, et al. Mining rice new germplasm containing S 5 n gene by functional molecular marker and sequencing. Chinese Sci Bull, 2009, 54: 3258–3264
Wang M X, Zhang H L, Zhang D L, et al. Geographical genetic diversity and divergence of common wild rice (O. rufipogon Griff.) in China. Chinese Sci Bull, 2008, 53: 3559–3566
Lu C, Pan X J. Studies on the wild compatibility inheritance of wild rice Dong-Ye and three cultivars 02428, 8504 and CPSL017 (in Chinese). Acta Agric Jiangxi, 1991, 3: 93–98
Liu J F. Rice widely affinity status of genetic research. Hybrid Rice, 1988, 3: 32–34
Zheng K L, Huang N, Bennett J, et al. RFLP-based phylogenetic analysis of wide compatibility varieties in Oryza sativa L. Theor Appl Genet, 1994, 88: 65–69
Zeng Y X, Hu C Y, Lu Y G, et al. Diversity of abnormal embryo sacs in indica/japonica hybrids in rice demonstrated by confocal microscopy of whole ovary. Plant Breed, 2007, 126: 574–580
Shi L G, Liu X D, Liu B, et al. Identifying neutral allele Sb at pollensterility loci in cultivated rice with Oryza rufipogon origin. Chinese Sci Bull, 2009, 54: 3813–3821
Jin J, Huang W, Gao J P, et al. Genetic control of rice plant architecture under domestication. Nat Genet, 40: 1365–1369
Yangihara S, Mccouch S R, Ishikawa K, et al. Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in Indica/Japonica hybrids of rice (Oryza sativa L.). Theor Appl Genet, 1995, 90: 182–188
Wan J, Yanagihara S, Kato H, et al. Multiple alleles at a new locus causing hybrid sterility between a Korean indica variety and a Javanica variety in rice (Oryza sativa L.). Jpn J Breed, 1993, 43: 507–516
Wan J, Ikehashi H. Identification of a new locus S-16 causing hybrid sterility in native rice varieties (Oryza sativa L.) from Tai-hu lake region and Yunnan Province, China. Breed Sci, 1995, 45: 461–470
Wan J, Yamaguchi Y Kato H, et al. Two new loci for hybrid sterility in cultivated rice (Oryza sativa L). Theor Appl Genet, 1996, 92: 183–190
Ikehashi H, Wan J. Differentiation of alleles at six loci for hybrid sterility in cultivated rice. In: IRRI, ed. Rice Genetics III. Manila, Philippines: IRRI, 1995. 409–417
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Wei, C., Wang, L., Yang, Y. et al. Identification of an S 5 n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility. Chin. Sci. Bull. 55, 1255–1262 (2010). https://doi.org/10.1007/s11434-010-0154-y
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DOI: https://doi.org/10.1007/s11434-010-0154-y