Abstract
Calcium and protein kinase serve as the common mediators to regulate plant responses to multiple stresses including salt and ABA stimulus. Here we reported a novel protein kinase (CIPK14) that regulated the responses to ABA treatment and salt stress in Arabidopsis. CIPK14 transcripts, capable been checked in roots, stems, leaves and flowers, were highly expressed in flowers and roots. CIPK14 was induced by ABA and salt treatments. The disruption of CIPK14 altered the transcriptional pattern of a gene marker line related to ABA and salt responses, and the results suggested that CIPK14 probably was responsible to the control of the salt and ABA responses. Comparing with wild types, the lines inserted with the T-DNA in which CIPK14 gene expression was knocked out were also more sensitive to ABA and salt stimulus, showing low germination rate and the less root elongation. While, when these conditioned seeds were treated with norflurazon, their germination percentages could recover to a certain extent. We also found that exogenous calcium could have an effect on the transcription of CIPK14 under ABA and salt treatments, and it seemed that calcium ion might work upstream CIPK14 to regulate the plant response to ABA and salt response.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Shi J R, Kim K N, Ritz O, et al. Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell, 1999, 11: 2393–2405 10590166, 10.1105/tpc.11.12.2393, 1:CAS:528:DC%2BD3cXkt1Cnsw%3D%3D
Harmon A C, Gribskov M, Harper J F. CDPKs: A kinase for every Ca2+ signal? Trends Plant Sci, 2000, 5(4): 154–159 10740296, 10.1016/S1360-1385(00)01577-6, 1:STN:280:DC%2BD3c3gslWntg%3D%3D
Zielinski R E. Calmodulin and calmodulin-binding proteins in plants. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49(NaN): 697–725 15012251, 10.1146/annurev.arplant.49.1.697, 1:CAS:528:DyaK1cXjvVSgs7Y%3D
Albrecht V, Ritz O, Linder S, et al. The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. EMBO J, 2001, 20: 1051–1063 11230129, 10.1093/emboj/20.5.1051, 1:CAS:528:DC%2BD3MXhvVemtb4%3D
Luan S, Kudla J, Rodriguez C M, et al. Calmodulins and calcineurin B-like proteins: Calcium sensors for specific signal response coupling in plants. Plant Cell, 2002, 14(suppl): S389–S400 12045290, 1:CAS:528:DC%2BD38XksV2gsr8%3D
Hrabak E M, Chan C W M, Gribskov M, et al. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol, 2003, 132(2): 666–680 12805596, 10.1104/pp.102.011999, 1:CAS:528:DC%2BD3sXkslertr8%3D
Cheong Y H, Kim K N, Pandey G K, et al. CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis. Plant Cell, 2003, 15(8): 1833–1845 12897256, 10.1105/tpc.012393, 1:CAS:528:DC%2BD3sXms1Wlsbw%3D
Pandey G K, Cheong Y H, Kim K H, et al. The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis. Plant Cell, 2004, July; 16: 1912–1924 15208400, 10.1105/tpc.021311, 1:CAS:528:DC%2BD2cXmtFSqtb4%3D
Kim K-N, Cheong Y H, Gupta R, et al. Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases. Plant Physiol, 2000, 124: 1844–1853 11115898, 10.1104/pp.124.4.1844, 1:CAS:528:DC%2BD3MXitVWqsg%3D%3D
Ishitani M, Liu J, Halfter U, et al. SOS3 Function in plant salt tolerance requires N-myristoylation and calcium binding. Plant Cell, 2000, 12: 1667–1678 11006339, 10.1105/tpc.12.9.1667, 1:CAS:528:DC%2BD3cXnsVyltrk%3D
Zhu J K. Salt and drought stress signal transduction in plants. Annu Rev Plant Biol, 2002, 53: 247–273 12221975, 10.1146/annurev.arplant.53.091401.143329, 1:CAS:528:DC%2BD38XlsVWhtbc%3D
Kim K N, Cheong Y H, Grant J J, et al. CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell, 2003, 15: 411–423 12566581, 10.1105/tpc.006858, 1:CAS:528:DC%2BD3sXhtlOmtbo%3D
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 1962, 15: 473–497 10.1111/j.1399-3054.1962.tb08052.x, 1:CAS:528:DyaF3sXksFKm
Yamaguchi-Shinozaki K, Shinozaki K. A novel cisacting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 1994, 6: 251–264 8148648, 10.1105/tpc.6.2.251, 1:CAS:528:DyaK2cXlslWqu70%3D
Kurkela S, Borg-Franck M. Structure and expression of kin2, one of two cold-and ABA-induced genes of Arabidopsis thaliana. Plant Mol Biol, 1992, 19: 689–692 1627780, 10.1007/BF00026794, 1:CAS:528:DyaK3sXktVCqurg%3D
Tahtiharju S, Sangwan V, Monroy A F, et al. The induction of kin genes in cold-acclimating Arabidopsis thaliana: Evidence of a role for calcium. Planta, 1997, 203: 442–447 9421928, 10.1007/s004250050212, 1:STN:280:DyaK1c%2FotlOmuw%3D%3D
Liu Q, Kasuga M, Sakuma Y, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and lowtemperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998, 10: 1391–1406 9707537, 10.1105/tpc.10.8.1391, 1:CAS:528:DyaK1cXls1SisLs%3D
Thomashow M F. Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol — Plant Mol Biol, 1999, 50: 571–599 15012220, 10.1146/annurev.arplant.50.1.571, 1:CAS:528:DyaK1MXkt1yktrw%3D
Zeevaart J A D, Creelman R A. Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol-Plant Mol Biol, 1988, 39: 439–473 10.1146/annurev.pp.39.060188.002255, 1:CAS:528:DyaL1cXltVOltrc%3D
Allen G J, Chu S P, Harrington C L, et al. Schroeder JI: A defined range of guard cell calcium oscillation parameters encodes stomatal movements. Nature, 2001, 411: 1053–1057 11429606, 10.1038/35082575, 1:CAS:528:DC%2BD3MXltVCru7w%3D
Kizis D, Pages M. Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through the droughtresponsive element in an ABA-dependent pathway. Plant J, 2002, 30: 679–689 12061899, 10.1046/j.1365-313X.2002.01325.x, 1:CAS:528:DC%2BD38Xls1ChtL0%3D
Niu X, Helentjaris T, Bate N J. Maize ABI4 binds coupling element1 in abscisic acid and sugar response genes. Plant Cell, 2002, 14: 2565–2575 12368505, 10.1105/tpc.003400, 1:CAS:528:DC%2BD38XotFelsLY%3D
Trewavas A J, Knight M R. Mechanical signalling, calcium and plant form. Plant Mol Biol, 1994, 26: 1329–1341 7858194, 10.1007/BF00016478, 1:CAS:528:DyaK2MXjvFelu7g%3D
McAinsh M R, Brownlee C, Hetherington A M. Calcium ions as second messengers in guard cell signal transduction. Physiol Plant, 1997, 100: 16–29 10.1111/j.1399-3054.1997.tb03451.x, 1:CAS:528:DyaK2sXkvFejsr8%3D
Rudd J J, Franklin T V E. Unravelling responsespecificity in Ca2+ signaling pathways in plant cells. New Phytol, 2001, 151:7–33 10.1046/j.1469-8137.2001.00173.x, 1:CAS:528:DC%2BD3MXltFGkurY%3D
Knight H, Knight M R. Abiotic stress signalling pathways: Specificity and cross-talk. Trends Plant Sci, 2001, 6: 262–267 11378468, 10.1016/S1360-1385(01)01946-X, 1:CAS:528:DC%2BD3MXlsFyjs7Y%3D
Xiong L, Zhu J K. Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell Environ, 2002, 25: 131–139 11841658, 10.1046/j.1365-3040.2002.00782.x, 1:CAS:528:DC%2BD38Xhslaktb0%3D
Klüsener B, Young J J, Murata Y, et al. Convergence of calcium signaling pathways of pathogenic elicitors and abscisic acid in Arabidopsis guard cells. Plant Physiol, 2002, 130: 2152–2163 12481099, 10.1104/pp.012187, 1:CAS:528:DC%2BD3sXktlyk
Lang V, Palva E T. The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh. Plant Mol Biol, 1992, 20: 951–962 1463831, 10.1007/BF00027165, 1:STN:280:ByyD1MbisVI%3D
Nordin K, Vahala T, Palva E T. Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol, 1993, 21: 641–653 8448363, 10.1007/BF00014547, 1:CAS:528:DyaK3sXisV2jsr0%3D
Ishitani M, Xiong L, Lee H, et al. HOS1, a genetic locus involved in cold-responsive gene expression in Arabidopsis. Plant Cell, 1998, 10: 1151–1161 9668134, 10.1105/tpc.10.7.1151, 1:CAS:528:DyaK1cXkvFejt7o%3D
Abe H, Yamaguchi-Shinozaki K, Urao T, et al. Role of Arabidopsis MYC and MYB homologs in drought-and abscisic acid-regulated gene expression. Plant Cell, 1997, 9: 1859–1868 9368419, 10.1105/tpc.9.10.1859, 1:CAS:528:DyaK2sXntFaks7s%3D
Shinozaki K, Yamaguchi-Shinozaki K. Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol, 2000, 3(3): 217–223 10837265, 1:CAS:528:DC%2BD3cXktVOnsrk%3D
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the “985” Program (China) for the higher education enhancement fund to Hunan University, Hunan Natural Science Foundation (Grant No. 05JJ30038) and National Natural Science Foundation of China (Grant No. 30600368)
Rights and permissions
About this article
Cite this article
Qin, Y., Li, X., Guo, M. et al. Regulation of salt and ABA responses by CIPK14, a calcium sensor interacting protein kinase in Arabidopsis. SCI CHINA SER C 51, 391–401 (2008). https://doi.org/10.1007/s11427-008-0059-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-008-0059-z