Abstract
Purpose
Nutrient release, soil wettability, water binding, and matrix rigidity of soil organic matter (SOM) can be affected by cross-links between segments of SOM, cations, and water molecule bridges (WaMB). Not all cation effects on SOM can be explained with the currently accepted idea that multivalent cations cross-link organic matter segments via direct cation bridges (CaB). The objective was to understand these interactions and their effect on SOM matrix rigidity and wettability.
Materials and methods
We modified cation composition of two peats and an organic surface layer (OSL) using cation exchange resin to remove cations and solutions of Na+, Ca2+, or Al3+ to enrich samples with cations. SOM matrix rigidity was determined at 4 and >8 weeks after treatment via the WaMB transition temperature T*, using differential scanning calorimetry. Wettability was measured via sessile drop contact angle (CA).
Results and discussion
The effect of cation removal on T* depended on cation exchange capacity and initial cation content. Cation addition to OSL increased T*. This effect increased with increasing cation loading and valency, and T* correlated with CA. Classical cross-linking can neither explain the higher heterogeneous matrix of Ca-treated than Al-treated samples nor the aging-induced convergence of T* for different cations and concentrations. The latter is likely due to interaction between CaB and WaMB in SOM.
Conclusions
Associations of CaB and WaMB evolve slowly and form a supramolecular network in SOM. Those dynamic associations can fix molecular arrangements inducing water repellency and increase kinetic barriers for the release and uptake of water and nutrients from aged soil.
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1 Introduction
Controlling chemical activity and release rates, solid soil organic matter (SOM) plays a central role for soil wettability and for availability of nutrients, water, and organic chemicals (Doerr et al. 2000; Pignatello 2012). Release and degradation of compounds can be controlled by physical entrapment in soil aggregates (Virto et al. 2011), molecular aggregates of humic substances (Li et al. 2013), or by kinetic barriers in the solid SOM matrix (Schaumann et al. 2000; Schaumann 2006). Rigid matrixes generally slow down transport of ions and molecules (Suvorova et al. 1999), e.g., glassy phases in natural organic matter (NOM) are made responsible for sequestration, sorption hysteresis, and increased desorption resistance of organic chemicals (LeBoeuf and Weber 2000a; Schaumann 2006). They can be softened by changes in temperature or moisture (LeBoeuf and Weber 2000a; Schaumann 2006), which can suddenly increase kinetic availability of any compound. Knowledge of the partly unknown mechanisms modifying matrix rigidity is essential for a profound understanding and prediction of pollutant and nutrient retention in soil (Schaumann and Bertmer 2008).
Up to now, two mechanisms of matrix softening have been demonstrated for NOM. Classical glass transitions (LeBoeuf and Weber 2000b) are induced by heating above the glass transition temperature, T g, in natural and model humic substances (LeBoeuf and Weber 2000b). Glass transitions are reversible transformations in amorphous regions between a hard and brittle state and a rubbery state (Seyler 1994). More relevant in SOM are water molecule bridge (WaMB) transitions, which are induced by the disruption of WaMB between molecular segments of SOM (Hurrass and Schaumann 2005; Schaumann and LeBoeuf 2005; Schaumann and Bertmer 2008; Aquino et al. 2009). WaMB resemble small water clusters pinned up between two functional groups and reduce mobility of molecular segments (Aquino et al. 2009). WaMB transitions are induced by heating above the WaMB transition temperature (T*) (Hurrass and Schaumann 2005; Schaumann and LeBoeuf 2005) or by changes in moisture conditions (Schaumann 2005; Schaumann and LeBoeuf 2005; Hurraß and Schaumann 2007). T* characterizes thermal stability of WaMB, which determines matrix rigidity in their surroundings (Schaumann and LeBoeuf 2005; Schaumann and Thiele-Bruhn 2011). Matrixes stiffened by WaMB undergo slow physicochemical aging, in which redistribution of water molecules increases the number and strength of WaMB and thus T* (Schaumann 2005; Hurraß and Schaumann 2007; Schaumann and Bertmer 2008). WaMB transitions in SOM have been observed and quantified by differential scanning calorimetry (DSC) and thermomechanical analysis (Schaumann et al. 2005; Schaumann and LeBoeuf 2005; Hurraß and Schaumann 2007) and demonstrated by 1H NMR wideline spectroscopy (Schaumann and Bertmer 2008; Jaeger et al. 2011). Exploring the currently available literature on WaMB transitions shows that up to now, the only available method to quantify T* is DSC.
Coordinative cross-links of molecular segments of organic matter (OM) via cation bridges (CaB) can also increase matrix rigidity (Lewis and Miller 1993). CaB have been suggested to explain cation effects on aggregate stability (Gaiffe et al. 1984), stability of SOM against degradation (Scheel et al. 2008), deceleration of release of dissolved organic carbon (DOC) in the presence of Ca2+ (Schaumann 2000), and increased mobilization of NOM in the presence of chelating agents (Yang et al. 2001). They can generate supramolecular associations of NOM molecules in aqueous environments (Piccolo 2002; Simpson et al. 2002; Wershaw 2004) and reduce molecular mobility in humic acids (HA) (Nebbioso and Piccolo 2009). Furthermore, aggregation of humic acid upon cation addition was found to be very sensitive to pH and the cation concentration (Tan et al. 2009). CaB were further used to explain sorption anomalies of organic chemicals in HA (Yuan and Xing 2001; Lu and Pignatello 2004) and SOM (Yang et al. 2001).
If CaB increase matrix rigidity, they can also alter SOM surface properties, as they can fix orientation of hydrophilic OM groups towards the cations in the interior, leading to partial charge compensation (Tan et al. 2011) and leaving the hydrophobic parts at the surface. Reorientation of the hydrophilic groups upon contact with water (Doerr et al. 2007; Diehl et al. 2010) would then be impeded by CaB with the consequence of increased wetting resistance.
The nature of CaB in SOM is not fully understood (Kunhi Mouvenchery et al. 2012). Cross-linking via direct CaB requires that functional groups are close enough to interact with one cation (Kunhi Mouvenchery et al. 2012). In SOM, not all functional groups fulfill this prerequisite. Larger distances could be bridged by a combination of CaB and WaMB (Aquino et al. 2011; Schaumann and Thiele-Bruhn 2011; Schaumann et al. 2013). The relevance of direct CaB, WaMB, and their interactions for matrix rigidity is unknown. Involvement of WaMB in CaB is highly relevant as it will lead to higher dynamics than direct CaB and may even overbalance individual cation effects. It is, therefore, essential to know to which extent classical cross-links, WaMB and CaB control matrix rigidity, and surface properties and how they interact with each other.
The objective of our study was to understand the role of WaMB in cation–SOM interactions and their effect on matrix and surface properties of SOM. In this first study, we exemplarily investigated the organic layer of a Haplic Podzol and two peat samples in interaction with Na+, Ca2+, and Al3+. Transfer to further soils and more cations requires additional studies. Ca2+ and Al3+ are known for their cross-linking characteristics, are typically present in soils with high SOM content like Podzols or Rendzinas, and we expect differences in their cross-linking efficiency due to their different valency. We tested the following hypotheses for cation effects: (1) Multivalent cations form cross-links between OM molecule segments and increase matrix rigidity. This increase is stronger for higher cation valency and for higher cation concentration (classical cross-linking), (2) cation-treated SOM reveals WaMB transitions with all typical characteristics including restricted reversibility and physicochemical aging, (3) the effect of WaMB overbalances the effect of CaB, and (4) CaB block reorientation of hydrophilic functional groups towards the surface of SOM and thus increase water repellency.
2 Material and methods
2.1 Samples, sample preparation, and soil analysis
We used two Histosols and a sample from an organic surface layer (OSL) of a Haplic Podzol. The OSL was sampled in a forest stand dominated by 80-year-old Norway spruces (Picea abies L. [Karst.]) in Southern Germany (thickness 0.15 m). The Histosol samples were taken from a Sapric Histosol from “Totes Moor” near Hannover (Jaeger et al. 2010) (peat 1; sampling depth 1.0–1.1 m) and from a Eutric Histosol from Heudorfer Ried, a meliorated fen in southwest Germany (Schneckenburger et al. 2012) (peat 2; sampling depth 0.0–0.2 m). The peat samples were air dried prior to use. OSL samples were shock frozen and stored at −18 °C. Shortly before experiments, the OSL samples were gently unfrozen in a fridge, air dried, and sieved to ≤1 mm. The effective cation exchange capacity (CECeff) was determined using barium chloride according to DIN ISO 11260 and DIN ISO 13536 (Gustafsson et al. 2003). pH was measured in 0.01 M CaCl2 at a soil/solution ratio of 1:2.5. Basic characteristics of the samples are summarized in Table 1.
2.2 Removal of cations via cation exchange resin treatment
In order to analyze the effect of cations naturally present in the samples, they were treated with cation exchange resin in order to extract large part of exchangeable cations (Kaupenjohann and Wilcke 1995; Lang and Kaupenjohann 2004). For the peats, one sample was extracted, and for the OSL, experiments were conducted in duplicates. Demineralization experiments were conducted with an acidic cation exchange resin (ion resin Amberlite® IR120, H+-Form, exchange capacity2.3 mmolc g−1, Merck, Darmstadt). Of the resin, 2.5 g was packed in a polypropylene net (mesh size 72 μm). Each resin bag was washed in 10% HNO3 for 30 min and rinsed with deionized water to remove DOC and free acid prior to use.
Two grams of sample and 50 ml of water were mixed for 15 min in a horizontal shaker to assure complete wetting. Resin bags were added and the suspension was treated in a horizontal shaker for 24 h. pH was monitored after 30 min and after 24 h. In contrast to Kaupenjohann and Wilcke (1995), pH adjustment was not necessary as it was close to the required pH 3. After 24 h, the resin bag was removed and rinsed with deionized water. The suspension was filtered (0.45 μm cellulose nitrate filters, Sartorius®) for DOC measurements. In order to extract the cations removed from the soil, resin bags were extracted in 50 ml HNO3 for 2 h. Extracts were analyzed for Ca2+ and Mg2+ via AAS (PerkinElmer 4100). Samples were stored for 4 weeks (Section 2.4) prior to DSC measurement.
2.3 Batch experiments for cation addition
Cation (Ca2+, Al3+, and Na+) addition experiments were conducted with the OSL sample. The peat samples had to be excluded due to reasons discussed as a first result of this study in Section 3. Ten grams of OSL was treated at pH 4 with 250 ml solutions of calcium nitrate, aluminum nitrate, and sodium nitrate. At this pH, Al3+ occurs as hydrated cation and hydroxide precipitates are not expected (Johnson 2002; Nierop et al. 2002). Seven different concentrations were prepared for Ca2+ (1, 2, 4, 8, 10, 16, and 20 mmolc l−1) and for Al3+ (0.15, 0.75, 1.5, 6, 12, 18, and 24 mmolc l−1) and three different concentrations for Na+ (10, 50, and 100 mmolc l−1). In preliminary experiments, these concentrations gave the best distribution between the original cation content and CECeff. Samples were prepared at least in duplicates. Suspensions were shaken for 3 h in a horizontal shaker. pH was monitored after 0.5 and 1.5 h and adjusted with 1 M HNO3 or 0.5 M NaOH to pH 4, if necessary. Then, the suspensions were filtered (589/2 ashless filter papers, Whatman). A subsample was treated in batch with deionized water as described above, to obtain a control sample without salt addition (named “control”).
2.4 Sample storage and physicochemical aging
After batch experiments, all samples were dried for 4 days at 25 °C in an oven and stored at 20 °C and 76% relative humidity (RH) over saturated sodium chloride solution in a desiccator. Aging effects were studied by DSC in all samples following isothermal storage at 20 °C and 76% RH for at least 8 weeks.
2.5 Chemical analysis of treated samples
To quantify the total amount of cations in the treated soil samples, two aliquots of each sample (1.5 g) were extracted with 40 ml 0.025 M ammonium ethylenediaminetetraacetate solution (99%, p.a., Carl Roth GmbH) at pH 4.6 for 3 h, followed by extraction using 1 M ammonium tetra acetate solution (10 min). The resulting extracts were analyzed for Ca2+, Al3+, Mg2+, Fe3+, and Mn2+ concentrations. Ca2+ and Mg2+ in extracts and supernatants were analyzed using flame AAS (PerkinElmer 4100), Fe3+ and Mn2+ were analyzed using graphite tube AAS (PerkinElmer 4100), and Al3+ was analyzed using a Varian AAS (AA 240 FS, N2O/acetylene flame). In addition, we analyzed the supernatants of all batch experiments (Section 2.3) to evaluate the removal of cations and dissolved organic matter (DOM) from the sample. Supernatants were analyzed for pH, Al3+, and Ca2+ and selected supernatants were additionally analyzed for Mg2+, Fe3+, and Mn2+, as described above. DOC was determined after filtration (0.45 μm Sartorius® cellulose nitrate filters pre-flushed with warm deionized water) using a TOC Analyzer Multi C/N 2100 (Analytik Jena) after acidification and outgassing of inorganic carbon.
2.6 Differential scanning calorimetry
DSC experiments were performed in order to quantify T* (Schaumann 2005; Schaumann and LeBoeuf 2005) with a DSC Q1000 (TA Instruments, Germany) using a refrigerated cooling system and nitrogen as purge gas. One- to three-milligram samples were placed in aluminum pans, which were hermetically sealed. Three to seven replicates per sample were measured depending on the thermogram quality. The temperature program involved abrupt cooling to −50 °C, heating with 10 K min−1 from −50 to 110 °C, followed by a second abrupt cooling and subsequent heating cycle. Baseline was corrected with the TZero technology® by TA Instruments. Data were analyzed using Universal Analysis 4.1 (TA Instruments).
DSC thermograms were checked for step transitions and for their reoccurrence in a second heating run. Step transitions occurring only in the first heating run were interpreted as WaMB transitions (Schaumann and LeBoeuf 2005). T* is indicated by the inflection point, which was determined by the application of three tangent lines, and the change of heat capacity (ΔC p) was calculated from the height of the central tangent line.
2.7 Sessile drop contact angle
The sessile drop CA was determined according to Diehl and Schaumann (2007). The sample was fixed on a glass slide (76 × 26 × 1 mm, Roth, Germany). Therefore, one side of the slide was covered by a double-sided adhesive tape (tesafix® 4934, tesa, Germany) and loaded with a layer of sample grains which was slightly pressed onto the tape with a spatula. Excess sample material was removed by carefully knocking the glass slide. The loading and removal of excess material was repeated three times to obtain a closed coverage by a one grain layer. At 20 °C, three to six drops of 100 μL demineralized water were placed on the sample layer. Drop shape was recorded from a side view of the drops by a digital camera (PowerShot A 300, Canon, Japan) posed exactly at the height of the sample layer. CA was determined after a contact time of 1 min fitting an ellipse to the drop contour, a baseline to the sample surface, and calculating the CA using the software SCA 20 (V3.10.7) from Dataphysics (Filderstadt, Germany).
2.8 Statistical analysis
Statistical analysis was performed using the software Origin 7.5 (OriginLab Corporation). Where linear trends were tested, we used linear regression. The probability given in the respective figures corresponds to that of the hypothesis that the slope is 0. Box plots of datasets of T* display their 25 and 75% percentile, their average, and their median. Differences between datasets were analyzed using one-way ANOVA.
3 Results and discussion
3.1 Soil properties
The OSL sample was acidic (pH 3.7) with CECeff of 303 mmolc kg−1 and contained 430 mg g−1 organic carbon (see Table 1). Peat 1 was more acidic (pH 2.7), contained considerably more organic carbon (539 mg g−1), but had a lower CECeff (123 mmolc kg−1), while peat 2 revealed low acidity (pH 5.8), contained less organic carbon (250 mg g−1), and had the highest CECeff (680 mmolc kg−1). The samples thus span a wide range of acidity, CECeff, and OM content and, therefore, likely contain OM differing in quality.
3.2 DSC thermograms
Representative DSC thermograms are shown in Fig. 1 for the OSL. The thermograms of the first heating cycle showed a step between 56 and 64 °C with ΔC p of 0.1-0.3 J g−1 K−1. The step disappeared in the second cycle and was thus interpreted as WaMB transition. T* was similar to that for samples of other peats and OSLs (Schaumann and LeBoeuf 2005; Schaumann et al. 2005).
Thermograms of peat 1 were more complex than those of peat 2 and OSL, consisting of an overlap of two processes in the same temperature region (data not shown), the nature of which is currently under investigation. One of these processes is non-reversing and fulfills the prerequisites for a WaMB transition. Due to the overlap, determination of T* leads for peat 1 to an uncertainty (ΔT*) of ±1 °C.
3.3 Effects of cation removal
Table 2 shows the effects of the resin treatment on the removal of cations with respect to the untreated samples. Ninety to 100% of Mg and Ca was removed from all soil samples. In peat 2, exchangeable Ca and Mg and their removal considerably exceeded the CECeff. Part of the Ca might have been present as soluble salts, or part of the Ca and Mg could have occupied single functional groups, with the consequence of local charge reversal neutralized by negatively charged counter ions. The idea that cation charge is not completely neutralized by functional groups in peat 2 is further supported by the strong pH decrease from 6.2 to 2.6 during resin treatment, corresponding to an increase in H+ concentration by a factor of 242. pH remains constant only if H+ released from the exchange resin can bind to exchange sites formerly occupied by cations (Lang and Kaupenjohann 2004).
In peat 1 and OSL, H+ concentration increased only by a factor of 15 (pH 4.0 → 2.8) and 45 (pH 4.4 → 2.8), respectively, and the amount of released cations did not exceed CECeff. Thus, large parts of the released cations were bound to two functional groups of OM in these samples.
Also, the effect of cation removal on T* differed between the samples (Fig. 2). While T* decreased for OSL and peat 1 by 2.5 and 1.5 °C, respectively, an increase by nearly 3 °C was observed for peat 2. Thus, removal of multivalent cations can either increase or decrease matrix rigidity, depending on the soil sample. The unexpected T* increase in peat 2 coincides with its initial cation supersaturation.
Thus, high Ca2+ contents destabilize the supramolecular OM matrix structure, while intermediate and low Ca2+ contents stabilize it. The T* cation content relation may thus have a maximum at a cation content indicating optimal cross-linking. In this view, the stronger decrease of T* upon demineralization of OSL than of peat 1 suggests that OSL was initially closer at its rigidity maximum. Alternatively, cross-linking potential in peat 1 could be lower than in OSL due its low CECeff.
Choice of samples for the cation addition approach
In the cation-overloaded peat 2, further cation addition will not change the OM cation saturation. Therefore, it was excluded from following experiments. As changes in T* of peat 1 are small and in the range of their large uncertainty due to the overlap of two processes, peat 1 was also excluded, but will be discussed in a separate study (Kunhi Mouvenchery et al. 2013).
3.4 Effect of cation addition on cation content and DOC loss of OSL
Cation content
In the Al- and in the Ca-treated OSL, cation content increased with increasing concentration of the salt solution and leveled out at a maximum (Fig. 3). The maximum Ca2+ content ranged between 270 and 300 mmolc kg−1 and was in the range of CECeff. Thus, all exchange sites accessible for Ca2+ were saturated with Ca2+. The content of exchangeable Mg2+ decreased from 40 mmolc kg−1 (control) to almost 0 mmolc kg−1 with increasing concentrations of Ca2+ in the solution, which shows that Ca2+ mainly replaces Mg2+ from its exchange sites. This observation is in line with studies reporting preference of SOM for the sorption of Ca2+ over Mg2+ due to the strongly held hydration shell of Mg2+ and the tendency of Ca2+ to form inner sphere complexes (Kalinichev and Kirkpatrick 2007). Besides Mg2+, some Mn2+ was replaced by Ca2+, indicated by a decrease of the Mn2+ content from 10 to 4 mmolc kg−1. Na+ preferentially replaced Ca2+ (decrease from 160 to 100 mmolc kg−1) and Mg2+ (decrease from 40 to 5 mmolc kg−1), but not Al3+ and Fe3+. In total, 95 mmolc kg−1 of Ca2+ plus Mg2+ ions was exchanged by Na+. Al3+ and Fe3+ contents remained unchanged irrespective the concentration of Ca2+ and Na+ in treatment solutions (data not shown).
In contrast, Al3+ uptake even exceeded CECeff for high Al concentrations. Inorganic Al precipitates are expected to be negligible below pH 4 (Johnson 2002; Nierop et al. 2002), but organic DOM-Al precipitates may have been formed or Al3+ might have exchanged cations not exchangeable with Ba2+. This assumption is supported by the exchange with Fe3+ observed only for the Al-treated samples, underlining the larger exchange capability of Al3+ than of any bivalent cation. Alternatively, single cation exchange sites without neighbors in sufficiently close proximity may be occupied by Al3+ accompanied by counterions. Furthermore, competition for exchange sites may additionally lead to occupation of formerly cross-linked exchange sites by two individual cations and therefore to a disruption of cross-links.
Loss of dissolved organic carbon
All treated samples exhibited considerably smaller DOC concentrations in the supernatant (1.5 ± 0.2 mg kg−1) than the control samples (2.0 ± 0.1 mg kg−1). DOC concentration tended to decrease with increasing initial cation concentration with slopes of −0.019 mg DOC (mmolc)−1 for Al3+ and -0.012 mg DOC (mmolc)−1 for Ca2+. Although not significant (P ≥ 0.1), this trend cannot be disregarded and is in line with other studies (Römkens and Dolfing 1998; Schaumann 2000). The strong efficiency of Al3+ to reduce DOC and thus to bind DOC fits well to the observation that Al3+ can decrease mineralization of DOC to up to 50% (Schwesig et al. 2003). In this view, the Al3+ contents in the OSL above CECeff could also be due to precipitation or flocculation of additional DOM from the treatment solution, resulting in a significant amount of Al–OM associates. In the Ca treatment, this DOM remained in the liquid phase.
3.5 Effect of cation addition on T*
After 4 weeks of storage, all controls revealed an average T* of 57.8 ± 0.4 °C. T* of the Na-treated samples averaged at 59.2 ± 0.6 °C independently of Na content. Ca and Al treatment resulted in T* of up to 60.0 and 61.6 °C, respectively. All T* values were in the range of T* normally found for organic soil samples (Hurrass and Schaumann 2005). The standard error of T* averaged 0.4 °C for the Al-treated samples and resembled that for other studies (Hurrass and Schaumann 2005). Ca-treated samples and Na-treated samples revealed larger standard errors (0.6–0.7 °C), which suggests higher heterogeneity of the matrix. T* of the Ca-treated samples and the Al-treated samples increased with increasing cation content (Fig. 4). This trend was significant for cation loadings up to 200 mmolc kg−1 (P < 0.05). Higher cation contents did not further increase T* significantly.
In order to compare the effects of all cation treatments although different in concentration dependence, we subdivided the data of the Ca-treated and Al-treated OSL into two groups: Concentration range 1 (“low”) refers to cation loadings below 200 mmolc kg−1, and concentration range 2 (“high”) refers to cation loadings above 200 mmolc kg−1. The resulting datasets are shown in box plots in Fig. 5a. Average T* was lowest in the demineralized sample (56.7 °C), followed by the control (57.8 °C), the Na- and Ca-treated samples (58.6–59.2 °C), and the Al-treated samples (low, 59.5 °C; high, 61.4 °C). Except for the Na-treated samples, most differences between the different treatments were significant (see Fig. 5a).
Figure 5b shows the cation effects on T*, calculated as difference between average T* of the treatment and that of the control. Demineralization significantly reduced T* by 1.1 °C, Na and Ca treatment resulted in a comparable increase of T* (increase of 0.8–1.5 °C), and Al treatment caused the strongest increase in T* (1.7–3.6 °C), especially for Al loadings above 200 mmolc kg−1.
These results demonstrate that (a) T* increases with cation loading for loadings below CECeff, (b) no further increase in T* occurs when the cation loading reaches CECeff, although cation loading can exceed CECeff, and (c) Al treatment results in a more homogeneous matrix than Ca2+ and Na+ treatment. In the light of the hypothesized CaB, this implies that cation addition increases the amount of CaB for low cation loadings. This effect increases with increasing cation loading and valency and is in line with the reduction of molecular mobility by Ca and Al observed with NMR for a HA (Nebbioso and Piccolo 2009). At higher loadings, cation addition can result in the reverse effect: CaB can be disrupted due to competition of numerous cations for exchange sites. This may oversaturate the exchanger and additionally bind anions into the matrix to maintain charge neutrality. In this situation, T* remains unaffected or even decreases with increasing cation saturation.
Up to this point, our data largely support the idea of direct CaB effective in the solid, air-dried organic matter, with a stiffening effect increasing with cation loading and valency. However, the higher heterogeneity of Na- and Ca-treated samples than of Al-treated samples needs further explanation. Al3+ can be more selective for certain cross-linking sites and immediately form inner sphere complexes with OM. For Ca2+ and Na+, the balance between energy gain due to formation of inner sphere complexes and energy required to release the hydration shell is not that clearly pronounced. Therefore, a larger variety of complexes is expected in the beginning, especially during cation treatment, ranging from outer sphere complexes (Aquino et al. 2011) to stable inner sphere complexes (Kalinichev and Kirkpatrick 2007) before aging and drying successively increases the percentage of inner sphere complexes and the relevance of short-range interactions.
3.6 Effect of aging on T*
To obtain further evidence for or against the classical cross-linking hypothesis, we investigated aging effects on T* of the solid OSL. Assuming direct CaB, aging should overcome initial sample heterogeneity and increase both T* and valency- and concentration-dependent differences in T*.
Sample storage resulted in a significant increase of T* for all samples compared to the nonaged samples (P < 0.05, Fig. 6). T* reached values of 60.5 °C for the control, 60.0 °C for the Na-treated OSL, 61.1–61.4 °C for the Ca-treated OSL and the Al-treated OSL (low), and 62.0 °C for Al-treated sample (high; see Fig. 6a). The standard error of T* after aging was 0.4 °C for all treatments, indicating homogenization of Na- and Ca-treated OSL. However, differences between the treatments decreased upon aging: Only for the aged Na-treated OSL and Al-treated OSL above 200 mmolc kg−1, T* still differed significantly from that of the aged control (T*Na − T*control = −0.5 °C and T*Al − T*control = +1.5 °C), indicating a flexibilization by Na and stiffening by Al in the aged sample. It cannot be excluded that initially observed cation and concentration dependence are successively canceled out with aging.
The aging effect on T* (T*i, aged − T*i, nonaged) ranged between +0.6 and +2.7 °C and was strongest for the control and for Ca treatment (see Fig. 6b). It was significantly smaller for Ca- and Al-treated samples with high cation loading (1.8 and 0.6 °C, respectively) than for low cation loadings (2.5 and 0.9 °C, respectively). It further increased with decreasing initial T* (P = 0.05; see Fig. 6c). Thus, initially least stable WaMB have the strongest aging effect.
Thus, some observations support classical cross-linking: T* increased upon aging, aged samples were more homogeneous, Na destabilized the aged matrix; and Al stabilized it. However, the differences between sample treatments decreased with aging time and the aging effect increased with decreasing initial T*. These observations contradict the classical cross-linking hypothesis.
The convergence of T* suggests that the aging process is due to a similar mechanism in all samples, independently of the cation. We assume that aging involves redistribution of water molecules leading to a successively increasing number and strength of WaMB (Schaumann and Bertmer 2008; Schaumann 2005). The decrease in the relevance of cation type and concentration with aging time suggests that the resulting supramolecular network in the OM is dominated by WaMB interacting with cations. The explanation that direct Al CaB form faster and more selectively than Ca CaB implies that further aging processes would be less effective for Al bridges because the favorable sites had already been occupied in the beginning. This is in line with the small aging effect in Al-treated samples.
If predominantly the WaMB network determines matrix rigidity, this would also explain the observation that aged Ca-treated samples exhibit T* values comparable to that of aged Al-treated samples and that heterogeneity of Ca-treated samples decreases with storage. During aging, redistribution of weakly bound Ca2+ ions and water molecules could increase the degree and strength of cross-linkage. Moreover, the cations can successively occupy sites which had been linked by water molecule bridges before cation treatment, and could undergo CaB associated with WaMB (Schaumann et al. 2013).
3.7 Effect of cation addition on contact angle
The CA of treated and untreated samples behaved in similar manner as T*. It increased with increasing Ca2+ and Al3+ content of the sample below 200 mmolc kg−1, flattened for higher Al3+ contents, and even decreased again for Ca-treated samples (Fig. 7a). As for T*, Al treatment was more effective than Ca treatment. Combining all CA data resulted in a clear linear correlation between CA and T* (P < 0.001; see Fig. 7b), although data points are grouped in three regions. This indicates that rigidity of the OM matrix is closely linked to wettability. Diehl and Schaumann (2007) identified a physical and chemical process responsible for changes in hydrophobicity of soil samples. The chemical process involves reorientation of hydrophilic OM groups on the surface towards the SOM interior where they are fixed by H bonds or ester linkages, resulting in higher surface hydrophobicity. In the same way, it can be assumed that cations fix functional groups and block their reorientation towards the outer surface.
4 Conclusions
Our results support the idea that multivalent cations form CaB and increase matrix rigidity in solid soil organic matter. However, only the short-term effects (4 weeks) are in line with the currently accepted classical cross-linking hypothesis. The convergence of T* upon aging for most cations and concentrations and the increase in aging effect with decreasing valency point to the relevance of WaMB interacting with CaB and increasing in strength. CaB–WaMB associations have been suggested only recently from NMR investigations of a peat (Schaumann et al. 2013). Considering that matrix rigidity acts as kinetic barrier for diffusion of ions and molecules, CaB and WaMB directly control kinetic availability of nutrients and organic chemicals in soil. As they are subjected to continuous dynamics, release of ions and molecules from soils will be slower under constant environmental conditions than after changes in cation or moisture status.
A second important outcome of this study is the observation that not only drying (Doerr et al. 2007), but also CaB alter hydrophobicity soils. Both effects arise from reorientation of hydrophilic functional OM groups towards the interior. CaB in the interior will block reorientation of functional groups towards the surface, with the consequence of higher wetting resistance and persistence of water repellency than due to sole drying-induced reorientation mechanisms.
Still, the transferability of the results of this study to other soils needs to be tested. The sample-dependent reaction of the WaMB transition temperature on cation removal suggests that other soils may give apparently conflictive results. The results of the cation addition study of peat 1 (Kunhi Mouvenchery et al. 2013) even suggest low relevance of CaB. This and our current findings suggest that the main difference in reaction of WaMB stability on cation treatment is determined by cation exchange capacity, which determines the amount and distances of potential cross-linking sites and the current cation loading of the sample. The knowledge of this interplay is highly relevant for the mechanistic understanding of functioning of biogeochemical interfaces under environmental conditions in the field.
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Acknowledgments
We acknowledge the financial support of the CROSSLINK project and the priority program SPP 1315 by the German Research Foundation (DFG, SCHA 849/6, and SCHA 849/8). Furthermore, we thank Susanne Woche and Jörg Bachmann for sampling of peat 1, Friederike Lang for providing the OSL sample, as well as for many helpful discussions, Sören Thiele-Bruhn and Tatjana Schneckenburger for providing the peat 2 sample and analyzing the Al samples, and Sabina Hens for her help in the laboratory.
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Schaumann, G.E., Gildemeister, D., Kunhi Mouvenchery, Y. et al. Interactions between cations and water molecule bridges in soil organic matter. J Soils Sediments 13, 1579–1588 (2013). https://doi.org/10.1007/s11368-013-0746-7
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DOI: https://doi.org/10.1007/s11368-013-0746-7