Summary
A lipase from Candida rugosa immobilized on styrene-divinylbenzene copolymer was used to catalyse the direct esterification of butanol and butyric acid. A factorial design was employed to evaluate the effects of temperature (37–50 °C), substrate molar ratio of butyric acid to butanol (0.6 to 2.0) and enzyme amount (0.2–0.4 g) on the ester yield. The main effects were fitted by multiple regression analysis to a linear model and maximum ester yield could be obtained working at 41 °C with 0.4 g of lipase. The mathematical model obtained, representing the ester yield has been found to describe adequately the experimental results. Under optimal conditions, concentration of 32.4 g butyl butyrate/l that corresponds to a yield of 75% was obtained.
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Santos, J.C., de Castro, H.F. Optimization of Lipase-catalysed Synthesis of Butyl Butyrate Using a Factorial Design. World J Microbiol Biotechnol 22, 1007–1011 (2006). https://doi.org/10.1007/s11274-005-2818-3
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DOI: https://doi.org/10.1007/s11274-005-2818-3