Summary
Glycerol has been known as an important by-product of wine fermentations improving the sensory quality of wine. This study was carried out with an endogenic wine yeast strain Saccharomyces cerevisiae Kalecik 1. The kinetics of growth and glycerol biosynthesis were analysed at various initial concentrations of glucose, fructose, and sucrose in a batch system. Depending on the determined values of Monod constants, glucose (K s = 28.09 g/l) was found as the most suitable substrate for the yeast growth. Initial glucose, fructose and sucrose concentrations necessary for maximum specific yeast growth rate were determined as 175 g, 100 l, and 200 g/l, respectively. The yeast produced glycerol at very high concentrations in fructose medium. Fructose was determined as the most suitable substrate for glycerol production while the strain showed low tendency to use it for growth. S. cerevisiae Kalecik 1 could not produce glycerol below 200 g/l initial sucrose concentration. When natural white grape juice was used as fermentation medium, maximum glycerol concentration and dry weight of the yeast were determined as 9.3 g/l and 11.8 g/l, respectively.
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References
G.P. Agarwal (1990) Microbial Bioproducts A. Fiechter (Eds) Advances in Biochemical Engineering Biotechnology Springer Germany 96–128
T. Deak L.R. Beuchat (1996) Handbook of Food Spoilage Yeasts CRC Press USA 209
S. Dequin (2001) ArticleTitleThe potential of genetic engineering for improving brewing, wine-making and baking yeasts Applied Microbiology and Biotechnology 56 577–588 Occurrence Handle10.1007/s002530100700 Occurrence Handle1:CAS:528:DC%2BD3MXnslGgs74%3D
R. Eustace R.J. Thornton (1987) ArticleTitleSelective hybridization of wine yeasts for higher yields of glycerol Canadian Journal of Microbiology 33 112–117 Occurrence Handle1:CAS:528:DyaL2sXhsFCks7s%3D Occurrence Handle10.1139/m87-019
R.E. Kirk D.F. Othmer (1999) Glycerol J. Kroschwitz (Eds) Concise Encyclopedia of Chemical Technology Interscience New York 1003–1004
M. Lambert A.C. Neish (1950) ArticleTitleRapid method for estimation of glycerol in fermentation solution Canadian Journal of Research 28 83–89
T. Omori K. Ogawa Y. Umemoto K. Yuki Y. Kajihara M. Shimoda H. Wada (1996) ArticleTitleEnhancement of glycerol production by brewing yeast (Saccharomyces cerevisiae) with heat shock treatment Journal of Fermentation and Bioengineering 82 187–190 Occurrence Handle10.1016/0922-338X(96)85048-3 Occurrence Handle1:CAS:528:DyaK28Xls1ekur8%3D
K. Özdamar (1999) Paket programlar ve istatistiksel veri analizi 1 Kaan Kitabevi Turkey 535
F. Remize Sablayrolles M. J. S. Dequin (2000) ArticleTitleRe-assessment of the influence of yeast strain and environmental factors on glycerol production in wine Journal of Applied Microbiology 88 371–378 Occurrence Handle10.1046/j.1365-2672.2000.00964.x Occurrence Handle1:CAS:528:DC%2BD3cXis1ektr8%3D
K.T. Scanes S. Hohmann B.A. Prior (1998) ArticleTitleGlycerol production by the yeast Saccharomyces cerevisiae and its relevance to wine: a review South African Journal of Enology and Viticulture 19 17–24 Occurrence Handle1:CAS:528:DyaK1cXntlSqsb8%3D
J.F.T. Spencer (1968) Production of polyhydric alcohols by yeasts D.J.D. Hockenhull (Eds) Progress in Industrial Microbiology Churchill Livingstone Edinburgh 2–42
M.J. Taherzadeh L. Adler G. Lidén (2002) ArticleTitleStrategies for enhancing fermentative production of glycerol – a review Enzyme and Microbial Technology 31 53–66 Occurrence Handle10.1016/S0141-0229(02)00069-8 Occurrence Handle1:CAS:528:DC%2BD38XksVansb4%3D
B.J. Viljoen G.M. Heard (2000) Saccharomyces cerevisiae R.K. Robinson C. A. Batt P.D. Patel (Eds) Encyclopedia of Food Microbiology Academic Press USA 1918–1924
Z.X. Wang J. Zhuge H. Fang B.A. Prior (2001) ArticleTitleGlycerol production by microbial fermentation: a review Biotechnology Advances 19 201–223 Occurrence Handle10.1016/S0734-9750(01)00060-X Occurrence Handle1:CAS:528:DC%2BD3MXlt1Smsb0%3D
J. Zhuge H.Y. Fang Z.X. Wang D.Z. Chen H.R. Jin H.L. Gu (2001) ArticleTitleGlycerol production by a novel osmotolerant yeast Candida glycerinogenes Applied Microbiology and Biotechnology 55 686–692 Occurrence Handle10.1007/s002530100596 Occurrence Handle1:CAS:528:DC%2BD3MXlt1KqsL8%3D
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Yalçin, S.K., Özbaş, Z.Y. Determination of Growth and Glycerol Production Kinetics of a Wine Yeast Strain Saccharomyces cerevisiae Kalecik 1 in Different Substrate Media. World J Microbiol Biotechnol 21, 1303–1310 (2005). https://doi.org/10.1007/s11274-005-2634-9
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DOI: https://doi.org/10.1007/s11274-005-2634-9