Abstract
Certain cost-effective carbohydrate sources in crude as well as after purification were utilized as the sole sources of carbon for gluconic acid production using Aspergillus niger ORS-4.410 under submerged fermentation. Crude grape must (GM) and banana-must (BM) resulted into significant levels of gluconic acid production i.e. 62.6 and 54.6 g/l, respectively. The purification of grape and banana-must led to a 20–21% increase in gluconic acid yield. Molasses as such did not favour gluconate production (12.0 g/l) but a significant increase in production (60.3 g/l) was observed following hexacyanoferrate (HCF) treatment of the molasses. Rectified grape must (RGM) appeared to be best suitable substrate which after 144 h resulted in 73.2 g of gluconic acid/l with 80.6% yield followed by the yield obtained from the rectified banana must (RBM) (72.4%) and treated cane molasses (TM) (61.3%). Abundant growth of mould A. niger ORS-4.410 was observed with crude grape (0.131 g/l/h) and banana must (0.132 g/l/h).
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
S. Anastassiadis A. Aivasidis C. Wandrey (2003) ArticleTitleContinuous gluconic acid production by isolated yeast-like mould strains of Aureobasidium pullulans Applied Microbiology Biotechnology 61 110–117
M. Bekers A. Vigants J. Laukevics M. Toma A. Rapoports P. Zikmanis (2000) ArticleTitleThe effect of osmo-induced stress on product formation by Zymomonas mobilis on sucrose International Journal of Food Microbiology 55 147–150 Occurrence Handle10.1016/S0168-1605(00)00186-0 Occurrence Handle10791734
P. Buzzini M. Gobbetti J. Rossi M. Ribaldi (1993) ArticleTitleUtilization of grape-must and concentrated rectified grape-must to produce gluconic acid by Aspergillus niger, in batch fermentation Biotechnology Letters 15 151–156 Occurrence Handle10.1007/BF00133015
C. Chen B.Y. Liu (2000) ArticleTitleChanges in major components of tea fungus metabolites during prolonged fermentation Journal of Applied Microbiology 89 834–839 Occurrence Handle10.1046/j.1365-2672.2000.01188.x Occurrence Handle11119158
C. Grassim P. Fauquembergue (1996) Fruit Juices T. Godfrey S.T. West (Eds) Industrial Enzymology Macmillan Press London 227–260
Holland, B., Welch, A.A., Unwin, I.D., Buss, D.H., Paul, A.A. & Southgate, D.A.T. 1997 In The Composition of foods, 5th edn, pp. 852–971. ed. McCane & Widdowson’s, Royal Society of Chemistry, Cambridge, ISBN 0–854-04428–0.
P. Kundu A. Das (1984) ArticleTitleUtilization of cheap carbohydrate sources for calcium gluconate production by Penicillium funiculosum mutant MN 238 Indian Journal of Experimental Biology 22 279–281 Occurrence Handle6480059
J.K. Lehman (1985) Comparative tests for fermentation H.J. Rehm G. Reed (Eds) Biotechnology. VCH Publishers Weinheimm 620–624
O.G. Lien (1959) ArticleTitleDetermination of gluconolactone, galactonolactone and their free acids by the hydroxamate method Analytical Chemistry 31 1363–1366 Occurrence Handle10.1021/ac60152a035
J.Z. Liu Y.Y. Huang J. Liu L.P. Weng L.N. Ji (2001) ArticleTitleEffects of metal ions on simultaneous production of glucose oxidase and catalase by Aspergillus niger Letters of Applied Microbiology 32 16–19 Occurrence Handle10.1046/j.1472-765x.2001.00851.x
F.G. Mann B.C. Saunders (1960) Practical Organic Chemistry Longman London 458–461
G. L. Miller (1959) ArticleTitleUse of dinitrosalicylic acid reagent for determination of reducing sugar Analytical Chemistry 31 426–428 Occurrence Handle10.1021/ac60147a030
D. Moonmangmee O. Adachi Y. Ano E. Shinagawa H. Toyama G. Theeragool N. Lotong K. Matsushita (2000) ArticleTitleIsolation and characterization of thermotolerant Gluconobacter strains catalyzing oxidative fermentation at higher temperatures Bioscience Biotechnology and Biochemistry 64 2306–2315 Occurrence Handle10.1271/bbb.64.2306
M. Petruccioli P. Piccioni M. Fenice F. Federici (1994) ArticleTitleGlucose oxidase, catalase and gluconic acid production by immobilized mycelium of Penicillium variabile P16 Biotechnology Letters 16 939–942 Occurrence Handle10.1007/BF00128629
S. Ray A.K. Banik (1999) ArticleTitleEffect of ammonium and nitrate ratio on glucose oxidase activity during gluconic acid fermentation by a mutant strain of Aspergillus niger Indian Journal of Experimental Biology 37 391–395 Occurrence Handle10641175
M. Roehr C.P. Kubicek J. Kominek (1996) Gluconic acid H.J. Rehm G. Reed (Eds) Biotechnology, Products of Primary Metabolism Weinheim VCH Publishers 347–362
T. Roukas (2000) ArticleTitleCitric acid gluconic acid production from fig by Aspergillus niger using solid-state fermentation Journal of Industrial Microbiology and Biotechnology 25 298–304 Occurrence Handle11320416
M.M. Silveira E. Wisbeck C. Lemmel G. Erzinger J.P. da Costa M. Bertasso R. Jonas (1999) ArticleTitleBioconversion of glucose and fructose to sorbitol and gluconic acid by untreated cells of Zymomonas mobilis Journal of Biotechnology 75 99–103 Occurrence Handle10553651
O.V. Singh B.M.J. Pereira R.P. Singh (1999) ArticleTitleIsolation and characterization of a potent fungal strain Aspergillus niger ORS-4 for gluconic acid production Journal of Scientific and Industrial Research 58 594–600
O.V. Singh A. Sharma R.P. Singh (2001a) ArticleTitleMutagenesis and production of gluconic acid by Aspergillus niger mutant ORS-4.410 in submerged and solid state surface cultivation Indian Journal of Experimental Biology 39 691–696
O.V. Singh A. Sharma R.P. Singh (2001) ArticleTitleOptimization of fermentation conditions for gluconic acid production by mutant Aspergillus niger Indian Journal of Experimental Biology 39 1136–1143 Occurrence Handle11906107
O.V. Singh R.K. Jain R.P. Singh (2003) ArticleTitleGluconic acid production under varying fermentation conditions by Aspergillus niger Journal of Chemical Technology and Biotechnology 78 IssueID2–3 208–212
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Singh, O.V., Kapur, N. & Singh, R.P. Evaluation of agro-food byproducts for gluconic acid production by Aspergillus niger ORS-4.410. World J Microbiol Biotechnol 21, 519–524 (2005). https://doi.org/10.1007/s11274-004-2395-x
Issue Date:
DOI: https://doi.org/10.1007/s11274-004-2395-x