Abstract
Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Birren, B. & Lai, E. 1993 Pulsed Field Gel Electrophoresis a Practical Approach. USA: Academic Press Inc., ISBN 0-12-101290-5.
Bouton, Y., Guyot, P. & Grappin, R. 1998 Preliminary characterization of microflora of Comte cheese. Journal of Applied Microbiology 85, 123–131.
Chef-DR II pulsed field electrophoresis systems, instruction manual and applications guide. Catalog numbers 170–3612 through 170– 3729, BioRad.
Carlson, C.R. & Kolsto, A.B. 1993 A complete physical map of a Bacillus thuringiensis chromosome. Journal of Bacteriology 175, 1053–1060.
Moschetti, G., Blaiotta, G., Villani, F. & Coppolo, S. 2001 Nisin producing organisms during traditional 'Fior di latte' cheese making monitored by multiplex PCR and PFGE analysis. International Journal of Food Microbiology 63, 109–116.
Olive, D.M. & Bean, P. 1999 Principles and application methods for DNA-based typing of microbial organisms. Journal of Clinical Microbiology 37, 1661–1669.
Rivera, A.M.G. & Priest, F.G. 2003 Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that relates in general to crystal protein gene content. FEMS Microbiology Letters 223, 61–66.
Tanskanen, E.I., Tulloch, D.L., Hillier, A.J. & Davidson, B. 1990 Pulsed field gel electrophoresis of Sma I digests of Lactococcal genomic DNA, a novel method of strain identification. Applied and Environmental Microbiology 56, 3105–3111.
Tenover, F.C., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H. & Swaminathan, B. 1995 Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of Clinical Microbiology 33, 2233–2239.
Vela, A.I., Vazquez, J., Gibello, A., Blanco, M.M., Moreno, M.A., Liebana, P., Albendea, C., Alcala, B., Mendez, A., Dominguez, L. & Fernandez-Garayzabal, J.F. 2000 Phenotypic and genetic characterization of Lactococcus garvieae isolated in Spain from Lactococcosis outbreaks and comparison with isolates of other countries and sources. Journal of Clinical Microbiology 38, 3791–3795.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yavuz, E., Gunes, H., Harsa, S. et al. Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli . World Journal of Microbiology and Biotechnology 20, 871–874 (2004). https://doi.org/10.1007/s11274-004-1004-3
Issue Date:
DOI: https://doi.org/10.1007/s11274-004-1004-3