Abstract
Porcine epidemic diarrhea virus (PEDV) LJB/03 was isolated from the fece of piglets infected with PEDV on a pig farm, Heilongjiang province, China. The M gene of LJB/03 was amplified from the RNA extracted directly from the fece samples by RT-PCR and cloned into pMD18-T vector. The M gene cDNA was sequenced and encompasses an open reading frame of 681 nucleotides, encoding a 226-amino acid protein. The LJB/03 M gene has a base composition of 152 adenines (22%), 153 cytosines (23%), 161 guanines (24%), and 214 thymines (31%). Sequence comparison with other PEDV strains selected from GenBank revealed that the LJB/03 M gene has a high sequence homology to those of other PEDV isolates, 97.80% with JMe2, 96.92% with KPEDV-9 (Korean field isolate), 97.36% with KPEDV-9 (Korean), 97.80% with Br1/87, and 97.94% with CV777. The encoded protein shared 97.79% amino acid identities compared with CV777, 97.35% with Br1/87, 97.79% with JMe2, 96.90% with KPEDV-9 (Korean field isolate), 96.46% with KPEDV-9 (Korean). Sequence analysis of the M gene, including genetic distance measurement, phylogenetic tree analysis, and residue substitution analysis, showed that all other PED viruses analyzed fell into three groups, and the LJB/03 itself branched in an independent group. These data revealed that the M gene nucleotide sequence of LJB/03 has some mutations in comparison with the other PED viruses.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Wu Liangxian (2000) Chinese FuJian J Anim Vet Sci 22 IssueID5 7
Wu Weizhong J Chinese Qinghai (2001) Anim Vet Sci 31 IssueID4 43–44
Ni Yanxiu Lin Jihuang He Kongwang Huan Honghua (2001) Anim Husbandry Vet Med 33 IssueID1 38–40
Barbara E. Straw., Sylvie D’llaire, William L. Mengeling, and David J. Taylor. Disease of Swine. 8th edn. Translated by Zhao Deming, Zhang Zhongqiu, Shen Jianzhong. 2000, pp. 181–187.
J.F. Timoney J.H. Gillespie F.W. Scott J.E. Barlough (1988) Hagan and Bruner’s Microbiology and Infectious Diease of Domestic Animals Comstock Publishing Associates Ithaca 897–898
A. Bridgen M. Duarte K. Tobler H. Laude M. Ackermann (1993) J Gen Husbandry Virol 74 1798–1804
M.B. Pensaert B.E. Straw S. D’Aallaire W.L. Mengeling D.I. Taylor (1999) Diease of Swine The Lowa University Press Ames, IA 179–185
Oldham, J., Pig Farming (Oct Suppl), 1972, pp. 72–73.
P. DeBouck P. Callebaut M. Pensaert (1982) Proc Int Congr Pig Vet Soc 7 53
M. Hofmann R. Wyler (1987) Schweiz Arch Tierheilkd 129 437–442 Occurrence Handle1:STN:280:DyaL1c%2Fhsl2htQ%3D%3D Occurrence Handle2821613
K. Mostl E. Horvath F. Burki (1990) Wien Tierarztl Monatsschr 77 10–18
Qinghua Cheng Niu Xiaoying Ye Chengyu (1992) Chinese Qinghai J Anim Vet Sci 22 IssueID3 22–23
Callebaut P. and DeBouck P., in Proc 5th. Int Congr Virol, Strasbonrg, p. 420.
P. DeBouck M. Pensaert (1980) Am J Vet Res 41 219–223 Occurrence Handle1:STN:280:DyaL3c7ntlCrtw%3D%3D Occurrence Handle6245603
A. Pijpers A.P. Van Nieuwstadt C. Terpstra J.H.M. Verheijden (1993) Vet Rec 132 129–131 Occurrence Handle1:STN:280:DyaK3s7ptVCmsQ%3D%3D Occurrence Handle8383370 Occurrence Handle10.1136/vr.132.6.129
E.N. Wood (1977) Vet Res 100 243–244 Occurrence Handle1:STN:280:DyaE2s3jsleksQ%3D%3D
F.A. Murphy E.P.J. Gibbs M.C. HorZinek M.J. Studdert (Eds) (1999) Veterinary Virology Academic Press San Diego 496–501
H.F. Egberink J. Ederveen P. Callebant M.C. Horzinek J Am (1988) Vet Res 49 1320–1324 Occurrence Handle1:CAS:528:DyaL1cXlsVGqsrk%3D
M. Knuchel M. Ackermann H.K. Muller U. Kihm (1992) Vet Microbiol 32 117–134 Occurrence Handle1:CAS:528:DyaK38XmtFShtLc%3D Occurrence Handle1441196 Occurrence Handle10.1016/0378-1135(92)90100-8
Yeo Sang-Geon Mercedes Hernandez Peter J. Krell Eva Nagy (2003) Virus Genes 26 239–246 Occurrence Handle10.1023/A:1024443112717
Sambrook, J., David W., and Russell, Molecular Cloning: A Laboratory Manual, 3rd edn. Translated by Huang Peitang. 2002, pp. 27–30.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jinghui, F., Yijing, L. Cloning and Sequence Analysis of the M gene of Porcine Epidemic Diarrhea Virus LJB/03. Virus Genes 30, 69–73 (2005). https://doi.org/10.1007/s11262-004-4583-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-004-4583-z