Abstract
The N-geneous method is a recently developed method for determination of low-density lipoprotein cholesterol (LDL-C) in human serum. In the present study, we attempted to adapt this method to bovine serum. The values of LDL-C obtained using the N-geneous method were highly correlated with those from the method using ultracentrifugation and heparin sepharose affinity chromatography (r = 0.934, p < 0.001). The reproducibility of this method was acceptable (intra-assay CV 4.2%, inter-assay CV 7.6%) for clinical use. Using the N-geneous method, serum LDL-C was evaluated in cows around parturition, and in cows with fatty liver induced by fasting. The concentration of LDL-C decreased significantly in cows close to parturition. A reduced concentration of LDL-C was also observed in cows with fatty liver. In both cases, the changes of LDL-C were similar to those of apolipoprotein B (apoB)-100, and the values of LDL-C were highly correlated (r = 0.876, p < 0.001) with those of apoB-100. These results suggest that the concentration of LDL-C reflects the level of apoB-100. The N-geneous method is simple and rapid, and might to be a useful tool to elucidate the clinical significance of LDL-C in bovine serum.
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Abbreviations
- apoB:
-
apolipoprotein B
- HDL:
-
high-density lipoprotein
- HDL-C:
-
high-density lipoprotein cholesterol
- LDL:
-
low-density lipoprotein
- LDL-C:
-
low-density lipoprotein cholesterol
- NEFA:
-
non-esterified fatty acid
- SRID:
-
single radial immunodiffusion
- TC:
-
total cholesterol
- TG:
-
triglyceride
- VLDL:
-
very lowdensity lipoprotein
References
Bauchart, D., 1993. Lipid absorption and transport in ruminants. Journal of Dairy Science, 76, 3864–3881
Bauchart, D., Durand, D., Laplaud, P.M., Forgez, P., Goulinet, S. and Chapman M.J., 1989. Plasma lipoproteins and apolipoproteins in the preruminant calf, Bos spp: density distribution, physicochemical properties, and the in vivo evaluation of the contribution of the liver to lipoprotein homeostasis. Journal of Lipid Research, 30, 1499–1514
Bland, J.M. and Altman D.G., 1986. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet, 1, 307–310
Burstein, M. and Scholnick, H.R., 1973. Lipoprotein–polyanion–metal interactions. Advances in Lipid Research, 11, 67–108
Carrol, D.J., Grummer, R.R. and Mao, F.C., 1992. Progesterone production by cultured luteal cells in the presence of bovine low- and high-density lipoproteins purified by heparin affinity chromatography. Journal of Animal Science, 70, 2516–2526
Cordle, S.R., Clegg, R.A. and Yeaman, S.J., 1985. Purification and characterization of bovine lipoproteins: resolution of high density and low density lipoproteins using heparin-Sepharose chromatography. Journal of Lipid Research, 26, 721–725
Folch, J., Lees, M. and Sloane Stanley, G.H., 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509
Friedewald, W.T., Levy, R.I. and Fredrickson, D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499–502
Ha, Y.C. and Barter, P.J., 1982. Differences in plasma cholesteryl ester transfer activity in sixteen vertebrate species. Comparative Biochemistry and Physiology B, 71, 265–269
Hatch, F.T. and Lees, R.S., 1968. Practical methods for plasma lipoprotein analysis. Advances in Lipid Research, 6, 1–68
Marcos, E., Mazur, A., Cardot, P. and Rayssiguier, Y., 1989. Quantitative determination of apolipoprotein B in bovine serum by radial immunodiffusion. Comparative Biochemistry and Physiology B, 94, 171–173
Marcos, E., Mazur, A., Cardot, P. and Rayssiguier, Y., 1990a. Serum apolipoprotein B and A-I and naturally occurring fatty liver in dairy cows. Lipids, 25, 575–577
Marcos, E., Mazur, A., Cardot, P. and Rayssiguier, Y., 1990b. The effect of pregnancy and lactation on serum lipid and apolipoprotein B and A-I. Journal of Animal Physiology and Animal Nutrition, 64, 133–138
Morrow , D.A., Hillman, D., Dade, A.W. and Kitchen, H., 1979. Clinical investigation of a dairy herd with the fat cow syndrome. Journal of the American Veterinary Medical Association, 174, 161–167
Oikawa, S. and Katoh, N., 1997. Reduced concentrations of apolipoproteins B-100 and A-I in serum from cows with retained placenta. Canadian Journal of Veterinary Research, 61, 312–314
Oikawa, S. and Katoh, N., 2002. Decreases in serum apolipoprotein B-100 and A-I concentrations in cows with milk fever and downer cows. Canadian Journal of Veterinary Reseach, 66, 31–34
Oikawa, S., Katoh, N., Kawawa. F. and Ono, Y., 1995. Decreased serum apolipoprotein B-100 and A-I concentrations in cows with ketosis and left displacement of the abomasum. American Journal of Veterinary Research, 58, 121–125
Rifai, N., Iannotti, E., DeAngelis, K. and Terence, L., 1998. Analytical and clinical performance of a homogeneous enzymatic LDL-cholesterol assay compared with the ultracentrifugation–dextran sulfate–Mg2+ method. Clinical Chemistry, 44, 1242–1250
Rudling, M.J. and Peterson, C.O., 1985. A simple binding assay for the determination of low-density lipoprotein receptors in cell homogenates. Biochimica et Biophysica Acta, 833, 359–365
Watson, E.D. and Williams, L.A., 1987. Influence of liver fat on postpartum hormone profiles in dairy cows. Animal Production, 45, 9–14
Yamamoto, O., Oikawa, S. and Katoh, N., 1995. Enzyme-linked immunosorbent assay for serum apolipoprotein B-100, a major triglyceride-transport protein in dairy cows. American Journal of Veterinary Research, 56, 1413–1417
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Miyamoto, T., Sugiyama, Y., Suzuki, J. et al. Determination of Bovine Serum Low-density Lipoprotein Cholesterol using the N-geneous Method. Vet Res Commun 30, 467–474 (2006). https://doi.org/10.1007/s11259-006-3276-6
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DOI: https://doi.org/10.1007/s11259-006-3276-6