Abstract
In this study, the genomic types of canine parvovirus (CPV) circulating in the State of Rio de Janeiro, Brazil, from 1995 to 2001, were investigated using the polymerase chain reaction assay (PCR). A total of 78 faecal samples from gastroenteritic puppies, confirmed as positive for canine parvovirus by haemagglutination/haemagglutination inhibition tests or virus isolation in cell culture (MDCK), were examined. The viral DNA was extracted from faecal samples using a combination of phenol– chloroform and silica–guanidine thiocyanate methods. PCR was carried out with differential pairs of primers to distinguish the old (CPV-2) and new types of virus (CPv-2a or CPV-2b). Specific amplicons were observed for all samples using the primer pair P2ab, which detects CPV-2a and CPV-2b. Seventy-six from a total of 78 samples (97%) were considered as CPV-2b because of their reaction with the primer pair P2b. Thirty samples (30/78) were from previously vaccinated puppies and in 15 of them the enteritis symptoms began from 1 to 12 days after vaccination. PCR confirmed the infection by wild virus (CPV-2b) in 5 of these 15 puppies who had received old-type vaccines. Our results show that CPV-2b was the prevalent type circulating in the State of Rio de Janeiro from 1995 to 2001.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CPV:
-
canine parvovirus
- FPLV:
-
feline panleukopenia virus
- HA:
-
haemagglutination
- MDCK:
-
Madin–Darby canine kidney cells
- PCR:
-
polymerase chain reaction
References
Alfieri, A.F., Alfieri, A.A., Barreiros, M.A.B., Leite, J.P.G. and Richtzenhain, L.J., 2004. G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996–1999. Veterinary Microbiology, 99, 167–173
Appel, M., Scott, F. and Carmichael, L.E., 1979. Isolation and immunization studies of a canine parvo-like virus fromdogs with haemorrhagic enteritis. Veterinary Record, 105, 156–159
Buonavoglia, C., Martella, V., Pratelli, A., Tempesta, M., Cavalli, A., Buonavoglia, D., Bozzo, G., Elia, G., Decaro, N. and Carmichael, L., 2000. Evidence for evolution of canine parvovirus type 2 in Italy. Journal of General Virology, 82, 3021–3025
Carmichael, L.E., Joubert, J.C. and Pollock, R.V., 1981. A modified-live canine parvovirus strain with novel plaque characteristics I: viral attenuation and dog response. Cornell Veterinary, 71, 784–791
Cubel Garcia, R.C.N., Pinto, A.M., Costa, A.P., Maciel, B.M., Oliveira, L.H.S., Nascimento, J.P., Santos, A.O., Castro, M.C.N., Willi, L.M.V. and Labarthe, N.W., 2000. Canine parvovirus infection in puppies with gastroenteritis in Niterói, Rio de Janeiro, from 1995 to 1997. Brazilian Journal of Veterinary Research in Animal Science, 37, 132–135
Cubel Garcia, R.C.N., Leite, J.P., Xavier, M.T.P., Castro, T., Mertens, R., Lemos, M. and Labarthe, N., 2002. Infecoãpelo parvovírus canino no Rio de Janeiro: um estudo de 5 anos. Revista Brasileira de Cieência Veterinária, 9, 42–46
Ganiere, J.P., Ruvoen, N., Gueguen, S., Hege, T., Chazel, M. and Aubert, A., 2000. Identification des types de parvovirus canin circulant en France. Revue Médicine Véterinaire, 151, 43–46
De Ybañez, R.R., Vela, C., Cortés, E., Simarro, I. and Casal, J.I., 1995. Identification of types of canine parvovirus circulating in Spain. The Veterinary Record, 136, 174–175
Greenwood, N.M., Chalmers, W.S.K., Baxendale, W. and Thompson, H., 1996. Comparison of isolates of canine parvovirus by monoclonal antibody and restriction enzyme analysis. Veterinary Record, 138, 495–496
Hirasawa, T., Yono, K. and Mikazuki, K., 1996. Detection and genomic analysis of canine parvovirus by the polymerase chain reaction. Journal of Veterinary Medicine, 43, 545–554
Ikeda, Y., Mochizuki, M., Naito, R., Nakamura, K., Miyazawa, T., Mikami, T. and Takahashi, E., 2000. Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virology, 278, 13–19
Kwok, S. and R. Higuchi. 1989. Avoiding false positives with PCR. Nature, 339, 237–238
Mochizuki, M., San Gabriel, M.C., Nakatani, H. and Yoshida, M., 1993. Comparison of polymerase chain reaction with virus isolation and haemagglutination assays for the detection of canine parvoviruses in faecal specimens. Research in Veterinary Science, 55, 60–63
Mochizuki, M., Horiuchi, M., Hiragi, H., San Gabriel, M.C., Yasuda, N. and Uno, T., 1996. Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia. Journal of Clinical Microbiology, 34, 2101–2105
Parrish, C.R., O'Connell, P.H., Evermann, J.F. and Carmichael, L.E., 1985. Natural variation of canine parvovirus. Science, 230, 1046–1048
Parrish, C.R., Have, P.D., Foreyt, W.J., Evermann, J.F., Senda, M. and Carmichael, L.E., 1988. The global spread and replacement of canine parvovirus strains. Journal of General Virology, 69, 1111–1116
Parrish, C.R., Aquadro, C.F., Strassheim, M.L., Evermann, J.F., Sgro, J.Y. and Mohammed, H.O., 1991. Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. Journal of Virology, 65, 6544–6552
Pereira, C.A.D., Monezi, T.A., Mehnert, D.U., D'angelo, M. and Durigon, E.L., 2000. Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay. Veterinary Microbiology, 75, 127–133
Sagazio, P., Tempesta, M., Buonavoglia, D., Cirone, F. and Buonavoglia, C., 1998. Antigenic characterization of canine parvovirus strains isolated in Italy. Journal of Virological Methods, 73, 197–200
Senda, M., Parrish, C.R., Harasawa, R., Gamoh, K., Muramatsu, M., Hirayama, N. and Itoh, O., 1995. Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines. Journal of Clinical Microbiology, 33, 110–113
Steinel, A., Venter, E.H., Van Vuuren, M., Parrish, C.R. and Truyen, U., 1998. Antigenic and genetic analysis of canine parvoviruses in Southern Africa. Onderstepoort Journal of Veterinary Research, 65, 239–242
Steinel, A., Munson, L., Van Vuuren, M. and Truyen, U., 2000. Genetic characterization of feline parvovirus sequences from various carnivores. Journal of General Virology, 81, 345–350
Truyen, U., Steinel, A., Bruckner, L., Lutz, H. and Möstl, K., 2000. Die verteilung der antigenen typen des caninen parvovirus in der Schweiz, Österreich und Deutschland. Schweizer Archiv für Tierheilkunde, 142, 115–119
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Costa, A.P., Leite, J.P.G., Labarthe, N.V. et al. Genomic Typing of Canine Parvovirus Circulating in the State of Rio de Janeiro, Brazil from 1995 to 2001 Using Polymerase Chain Reaction Assay. Vet Res Commun 29, 735–743 (2005). https://doi.org/10.1007/s11259-005-3865-9
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11259-005-3865-9