Abstract
Biological soil crusts are common in many arid and semi-arid regions and they can alter microenvironments which are likely to directly and indirectly influence vascular plant establishment. The effect of biological soil crusts on germination is also influenced by the biological characteristics of the seeds themselves but rarely have the effects of both crust type and seed morphology on germination been examined in the same study. In this study, seed of five semi-arid woodland species with contrasting seed morphology were sown on top of patch types that commonly occur in natural woodlands (foliose lichen, short-turf moss, tree leaf litter, disturbed crust) and their emergence was followed. Percent germination varied between patch types and, for the largest-seeded species (Maireana excavata), final germination was significantly lower on the biological soil crust and litter patch types because they strongly acted as a physical barrier to seed penetration into the soil. The remaining four species showed no significant differences in final percent germination with patch type because most seeds either completely or partially penetrated the surface layer. Germination time courses, however, showed that biological soil crusts delayed the timing of germination of these species. Hence, soil crusts might differentially affect the spatial patterning of species in semi-arid woodlands by their subtle influence on seedling emergence that is determined by differences in seed morphology and subsequent positioning within crusts.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
Biological soil crusts consisting of lichens, bryophytes, algae, cyanobacteria and fungi are a common and widespread feature of arid and semi-arid landscapes worldwide. In many areas, they are considered to be essential components of healthy ecosystems and several studies have addressed aspects of the influence of crusts on vascular plants (West 1990; Eldridge and Greene 1994; Belnap 2002, Escudero et al. 2007; Su et al. 2007). The available data, however, indicate that biological soil crusts can have either positive (Hawkes 2004; Rivera-Aguilar et al. 2005), inhibitory (Deines et al. 2007; Serpe et al. 2006) or no effect on vascular plant establishment and cover (Prasse and Bornkamm 2000; Belnap et al. 2001).
Positive effects of biological soil crusts on vascular plant establishment are supported in a variety of environments by observational correlations between vascular plant cover and the area occupied by crusts (Lesica and Shelly 1992; Eldridge 1993; Bliss and Gold 1999). Experiments under laboratory conditions have also shown positive effects of crusts on germination (e.g. St Clair et al. 1984; Hawkes 2004; Rivera-Aguilar et al. 2005). Hawkes (2004) noted that germination was several fold higher on crusted than on bare soils. Positive effects of the crust on vascular plants may be the result of moisture conditions created by the crust, improved nutrient availability, decreased predation or enhanced seed lodgement (Van Tooren 1988; Van Tooren 1990; Belnap and Harper 1995; Belnap et al. 2001; Su et al. 2007).
Negative correlations between vascular plant cover and the presence of biological soil crusts have also been reported. These correlations have occurred on different types of crust including lichen-dominated, moss-dominated and cyanobacterial crusts (Keizer et al. 1985; Van Tooren 1990; Eldridge et al. 2000; Prasse and Bornkamm 2000; Sedia and Ehrenfeld 2003). Experiments in controlled environments have also shown that the presence of biological soil crusts can inhibit the germination of some species (Zaady et al. 1997; Zamfir 2000; Serpe et al. 2006; Escudero et al. 2007; Deines et al. 2007). Negative effects of the crust on germination could be attributed to competition for water and nitrate, changes in the red/far-red ratio, presence of a physical barrier to root penetration or the presence of inhibitory compounds such as metabolites with allelopathic properties (Van Tooren et al. 1985; Johansen 1993; Belnap et al. 2001; Deines et al. 2007).
The various effects of biological soil crusts on germination are likely to reflect differences in crust composition, seed characteristics and environmental conditions (Prasse and Bornkamm 2000; Zamfir 2000; Serpe et al. 2006; Deines et al. 2007; Su et al. 2007). Differences in crust composition can result in crusts with various morphological, physical and chemical characteristics resulting in different seedbed environments (Eckert et al. 1986; Eldridge and Rosentreter 1999; Belnap et al. 2001; Serpe et al. 2006; Escudero et al. 2007). Furthermore, seeds vary in their requirements for germination and consequently, may respond differently to the conditions created by the crust (Zaady et al. 1997). Evidence is accumulating to suggest that the interaction between crusts and germination of vascular plants is influenced by essential biological characteristics of seeds, such as size, shape and structure (Johansen 1993; Eldridge and Greene 1994; Zaady et al. 1997; Zamfir 2000; Sedia and Ehrenfeld 2003; Morgan 2006; Deines et al. 2007). For example, biological soil crusts may inhibit germination of seeds that lack burial mechanisms or structures that facilitate soil–seed contact (Van Tooren 1990; Belnap et al. 2001). In addition, the crust may only affect germination under certain environmental conditions such as when germination is limited by water deficits (Keizer et al. 1985; Rokich and Bell 1995; Zamfir 2000; Hawkes and Menges 2003; Hawkes 2004; Li et al. 2005).
Because biological soil crusts may favour the establishment of some plants over others, their presence is likely to influence the distribution of vascular plants (Belnap 2003). Biological soil crusts, though important, do not completely dominate the ground layer in all crust associated ecosystems (Martinez et al. 2006; Thompson et al. 2006). Litter can also be an important ground cover component (Facelli and Pickett 1991), as are localised animal diggings that disrupt soil crusts and litter (Pyrke 1994). Therefore, whilst it is useful to study the interaction of biological soil crusts and vascular plants alone, it is likely to be more beneficial to also consider the role of litter and disturbance in generating soil patch-type heterogeneity and how plants, via seed germination, respond to such patchiness.
In semi-arid woodlands, crusts are common components (Eldridge 1996; Belnap 2003) but their ecological role in Australia has rarely been examined. Previously, we identified the diversity and distribution of crust components across a topography/community gradient in a semi-arid environment (Briggs and Morgan 2008) and we now ask: do these crusts affect the potential distribution of vascular plant species by affecting germination? In this study, the effects of biological soil crusts (intact and disturbed) and litter on germination of five species (covering a range of seed characteristics) from semi-arid woodlands in southern Australia were examined in a laboratory experiment.
Methods
Study area
Soil samples for this experiment were collected from Terrick Terrick National Park (TTNP), 225 km northwest of Melbourne, Victoria, Australia. TTNP is situated within the Patho Plains, a sub-section of the Victorian Northern Plains Bioregion, and includes the Terrick Terrick Range and adjacent plain. Average annual precipitation for the Patho plains is ~400 mm, with winter months weakly dominating the rainfall patterns. Seasonal average maximum temperatures range from 30°C in summer to 15°C in winter. Seasonal average minimums range from 15°C in summer to 3°C in winter. The vegetation is a semi-arid Callitris grassy woodland typical of the rocky outcrops and outwash slopes at TTNP.
Study species
Five species common in semi-arid woodlands at TTNP and covering a range of seed sizes, shapes and germinability were selected for study: a Salvia verbenaca (Lamiaceae), Austrodanthonia sp. (Poaceae), Maireana excavata (Chenopodiaceae), Leptorhynchos scaber (Asteraceae) and Vittadinia gracilis (Asteraceae). Table 1 and Fig. 1 provide a description of the habit, seed morphology and mass and germinability of these species. [a denotes exotic species].
Effect of patch type on germination
This experiment was designed to compare germination on different patch types amongst species. Seeds were collected from TTNP as they matured (September–October 2006) and stored dry at room temperature. At the beginning of November 2006, 48 naturally occurring soil ‘patch type’ samples were excavated from Callitris-dominated woodlands. Twelve patches (5 cm deep × 17 cm wide × 25 cm long) each of foliose lichen-dominated (Xanthoparmelia sp.), mixed-species short turf moss-dominated, Callitris leaf litter-dominated (average depth 7 mm) and disturbed soil crust (raked with a hoe to break up crust and surface soil) were removed whilst the soil was moist, placed in plastic trays and transported to La Trobe University where they were allowed to dry at room temperature for 7 weeks prior to commencement of the experiment. The minimum cover of foliose lichen and moss on a sample was approximately 50% and 60%, respectively. A thin cyanobacterial crust was present underneath the moss and lichen dominated patch samples.
In late December 2006, 15 seeds each of S. verbenaca, Austrodanthonia sp., L. scaber, V. gracilis and, due to their larger size, 10 seeds of M. excavata, were placed onto the surface of patch types, giving 12 replicates per treatment for each species. For foliose lichen and moss samples not covering 100% of the surface, seeds were specifically placed on top of the particular soil crust component. All seeds were placed on the surface rather than being buried, with the aim of mimicking initial positions of seeds after wind dispersal. The trays were then placed into two Zankel germination chambers (six replicates of each treatment in each chamber) set at 12 h light/12 h dark and 20/10°C diurnal light and temperature cycles, respectively. On day one of the experiment, each soil surface replicate was sprayed with 200 ml of distilled water reflecting a 12 mm rainfall event. Replicates were then watered to soil saturation (approximately 300 ml) on day five and this 5-day-watering regime was maintained for the duration of the experiment (60 days). This regime allowed drying out of soil surface between watering. Every five days, treatments were sprayed with distilled water, trays re-randomized within the germination chambers, and seeds checked for germination. Seeds were considered germinated on emergence of the radicle, or appearance of the cotyledon.
Seed fate
To determine if differences in germination were related to seed position within the ground cover layer, at the conclusion of the experiment the positional fate of every seed (germinated and un-germinated) on each patch type was assessed and placed into one of three categories: (1) no penetration (NP)—no part of the seed was below the surface layer of the patch type in question; (2) partial penetration (PP)—some part of the seed was below the surface layer, and; (3) complete penetration (CP)—the whole seed was below the surface layer.
Data analysis
Germination lag time (LAG) was recorded as the time from start of experiment until germination commenced. Germination speed (T 50) was calculated as the time taken from commencement of germination until 50% of the final percent germination was reached. The influence of patch type treatment and germination chamber effects on final percent germination were tested for each species using a Nested ANOVA, with germination chambers nested within treatments. Bonferroni post-hoc comparisons were used when significant treatment effects (P < 0.05) were detected. Normality was visually assessed using histograms and Levene’s test was used to check homogeneity of variances. If the Levene’s test proved significant, data were transformed (arcsine, square root and fourth root). If the test remained significant after transformation, the non-parametric Kruskal–Wallis test was utilised with post-hoc comparisons made using Mann–Whitney U-tests.
The Kaplan–Meier method was used to assess differences in germination rates (Pyke and Thompson 1986). This method (traditionally used in survival analysis) allows the use of germination data consisting of elapsed time from the beginning of the experiment to seed germination (non-censored data), but also right-censored data (i.e. seeds that did not germinate before the end of the experiment). Pairwise shape comparisons for modelled germination curves were tested using Mantels Log Rank test (Pyke and Thompson 1986).
Seed fate was analysed using one way analysis of variance and Bonferroni post-hoc comparisons, using the NP (no penetration) data which, intuitively, represents evidence for soil crusts acting as a physical barrier to germination. All three seed fate categories were not subject to statistical testing because they experience an inverse relationship. Normality and homogeneity of variances were assessed as above for final percent germination, and the Kruskal–Wallis test was applied to appropriate data.
Effect sizes of moss, foliose lichen and litter treatments were calculated relative to the disturbed (bare soil) treatment which acted as the control for this analysis (Quinn and Keough 2002). Effect sizes can be interpreted by comparing percent of non-overlap of the treated group’s scores with those of the untreated group (Cohen 1988). An effect size of 0.0 indicates that the distribution of scores for the treated group overlaps completely with the distribution of scores for the untreated group, i.e. there is 0% of non-overlap. An effect size of 0.8 indicates a non-overlap of 47.4%, and an effect size of 1.7 specifies a non-overlap of 75.4% in the two distributions (Cohen 1988).
The Kaplan–Meier method was performed using the software package Systat version 10. All other statistical analysis was undertaken in SPSS version 14.0
Results
Effect of patch type on germination
Germination chamber effects
No significant effect of germination chamber or germination chamber × treatment interaction was detected in any of the analyses (P > 0.05 for all species). Therefore, differences observed were attributed to the patch type treatment and those effects are reported below.
Germination success
Final percent germination varied both amongst and within species across patch types (Table 1). a Salvia verbenaca had the highest percent germination on all patch types (except disturbed, on which M. excavata exhibited the highest germination) (Table 1). Germination of L. scaber was generally low on all substrates (<3%) (Table 1) and hence, was excluded from all statistical analysis. Final percent germination of M. excavata was significantly higher, by an order of magnitude, on disturbed soil than on litter, moss and foliose lichen patches (Table 1). V. gracilis germination was not significantly affected by patch type (P > 0.05), and small effect sizes support this result (Fig. 2d). Final percent germination of a S. verbenaca and Austrodanthonia sp. was not significantly different amongst patch types (Table 1). However, moss inhibited germination of Austrodanthonia sp. by an effect size of −1.14 (relative to disturbed control, Fig. 2b) and facilitated germination of S. verbenaca by an effect size of 0.58 (Fig. 2a).
Germination lag and speed
Patch type affected lag time and germination speed (T 50) of all species (Table 1). All species experienced the shortest lag time on the disturbed soil crust, with a S. verbenaca, Austrodanthonia sp. and V. gracilis commencing germination within 10–15 days, and M. excavata commencing within 20–25 days (Table 1). a Salvia verbenaca and V. gracilis exhibited delayed germination on the foliose lichen patch type (20–25 and 30–35 days, respectively), whilst moss patches incurred the longest lag time of Austrodanthonia sp. and M. excavata (45–50 and 50–55 days, respectively) (Table 1). a Salvia verbenaca and V. gracilis exhibited the shortest T 50 on disturbed soil crust and longest on moss and foliose lichen (Table 1). T 50 was shortest on moss for Austrodanthonia sp. and M. excavata.
Germination time courses
Germination time courses varied between patch types for each species (Fig. 3). Germination curves obtained via the Kaplan–Meier method were significantly different amongst patch types for a S. verbenaca (Mantel χ2 = 16.22, df = 3, P = 0.001), Austrodanthonia sp. (Mantel χ2 = 11.08, df = 3, P = 0.011) and M. excavata (Mantel χ2 = 129.10, df = 3, P < 0.001). The a S. verbenaca germination curve obtained on the moss patch type differed significantly from curves obtained on the disturbed (Mantel χ2 = 4.70, df = 1, P = 0.030), litter (Mantel χ2 = 10.95, df = 1, P = 0.001) and foliose lichen patch types (Mantel χ2 = 12.67, df = 1, P < 0.001). This was also the case for Austrodanthonia sp. with the curve from moss differing significantly to the curves from disturbed (Mantel χ2 = 9.72, df = 1, P = 0.002), litter (Mantel χ2 = 8.64, df = 1, P = 0.003) and foliose lichen (Mantel χ2 = 4.49, df = 1, P = 0.034). The disturbed patch type significantly altered the germination curve of M. excavata relative to litter (Mantel χ2 = 46.38, df = 1, P < 0.001), moss (Mantel χ2 = 43.85, df = 1, P < 0.001) and foliose lichen (Mantel χ2 = 54.83, df = 1, P < 0.001). Maireana excavata germination curves from the moss and foliose lichen patch types also differed significantly (Mantel χ2 = 4.06, df = 1, P = 0.044), though the effect was weak. Vittadinia gracilis showed no significant difference between germination curves from different patch types (Mantel χ2 = 1.39, df = 3, P = 0.709).
Seed fate
Seed fates on each patch type varied amongst species (Fig. 4). The percent of seeds not penetrating the surface differed significantly between patch types for all species (a S. verbenaca H = 31.64, P < 0.001; Austrodanthonia sp. H = 21.80, P < 0.001; M. excavata F = 27.93, P < 0.001; L. scaber H = 8.57, P = 0.036; V. gracilis F = 6.05, P = 0.002) (df = 3 for all species). Moss, litter and foliose lichen inhibited penetration of M. excavata. Significantly more seeds did not penetrate these patch types than did not penetrate disturbed soil (Fig. 4c). In other words, a greater percentage of M. excavata seeds penetrated (partially or completely) disturbed soil. For Austrodanthonia sp., complete penetration was greatest on disturbed soil and litter (64% and 75%, respectively), whilst the percentage of seeds not penetrating moss was significantly greater than the percentage of seeds not penetrating the other patch types (Fig. 4b). a Salvia verbenaca and L. scaber seeds all penetrated disturbed soil to some extent (Fig. 4a, d), and in the case of the latter species, this resulted in a significant difference between the percent of seeds not penetrating disturbed soil and the three other patch types. However, overall percentages were low (<7%) in that seed fate category. The majority of V. gracilis seeds partially penetrated all patch type surfaces (Fig. 4e), but the percentage of seeds not penetrating the moss surface was significantly greater (P < 0.05) compared to the litter surface.
Discussion
The results of this study indicate that patch type can differentially influence germination of plants found in semi-arid woodlands. However, outcomes of patch type vary and may reflect differences in seed size and structure, and the germinability of the species. Overall germination was highest for a S. verbenaca and Austrodanthonia sp., the two species with mechanisms for enhancing seed–soil (moisture) contact. The round seeds of a S. verbenaca develop a thick mucilage which increases seed–water contact, whilst Austrodanthonia sp. possess a hygroscopic awn which enhances the seed’s ability to penetrate the surface layer. Moreover, biological soil crusts may have more influence on germination time courses than on final percent germination.
Germination of all species occurred first on disturbed patches, probably because seeds in close contact with the soil typically have a greater and more reliable supply of moisture than seeds on the soil surface (Rokich and Bell 1995). Hence, water uptake and germination are faster (Oomes and Elberse 1976). The effects were especially pronounced for the large disc-shaped seeds of M. excavata (Fig. 1), which showed significantly higher final percent germination on disturbed patches compared to biological soil crust and litter patches. M. excavata has a high germinability and is likely limited by moisture availability. Although moss patches can enhance moisture availability (Van Tooren et al. 1985), seeds of M. excavata did not benefit because their large size prevented them from penetrating the moss layer. By contrast, the smaller seeds of a S. verbenaca were able to penetrate the surface and germination was promoted, most likely as a result of increased moisture. This suggests that due to the seed size and structure of M. excavata, foliose lichen, litter and moss patch types indirectly prevented germination by acting as a physical barrier to the soil or to microsites where conditions may be more favourable. Morgan (2006) also showed that large-seeded species did not germinate on top of soil crusts whereas small-seeded species germinated in small quantities. The regeneration niche for species such as M. excavata may be promoted by the soil disturbances created by native digging animals (Pyrke 1994).
Short-turf moss delayed and decreased germination of the grass Austrodanthonia sp. relative to the disturbed treatment (though the difference was not significant, the effect size was great). Serpe et al. (2006) found similar results on short moss-dominated crust relative to bare soil for four grass species in the Great Basin, USA. Seeds of Austrodanthonia sp. possess a hygroscopic awn and, upon wetting can essentially ‘drill’ their way into cracks and depressions. Although germination on foliose lichen and litter patch types was initially reduced, once seeds were positioned within or beneath the surface, they were more likely in a moister microenvironment, and this process may explain the delayed germination. This theory is supported by seed fate data which show similar percentages of seeds penetrated litter, foliose lichen and disturbed soil patches, whilst the percent penetrating short-turf moss patches was significantly lower than these patch types.
aSalviaverbenaca germination on disturbed soil crust peaked at around 20% by day 30, with little germination after this time. Almost 100% of aS. verbenaca seeds completely penetrated the surface of disturbed soil and observations suggest burial into the soil occurred quite rapidly (i.e. in the first 25 days of the experiment). Seeds generally have optimal burial depths, and increased or decreased burial can prevent germination (Baskin and Baskin 1998). Therefore, it is possible further germination was not seen on this patch type because seeds eventually became buried too deeply, hampering gas exchange and blocking light. The percentage of aS. verbenaca seeds which partially penetrated the surface was highest on moss patches, suggesting this position is most favourable for germination. In chalk grasslands, the presence of a bryophyte layer enhances water availability at the soil surface (Van Tooren et al. 1985); similarly, mosses in the present study are likely to create a moister microenvironment than that of disturbed soil and aS. verbenaca seeds can further optimise conditions through their mucilage layer. Partial penetration of moss combines advantages of increased moisture availability with maintenance of full (or close to full) exposure to light which may be a requirement for germination of this species.
Vittadinia gracilis is abundant across the study area. This species germinated equally well under all patch type treatments and germination time courses were not significantly different. Seed fate of this species was similar across patch types with only moss and litter differing significantly in the number of seeds not penetrating the surface. The majority of seeds partially penetrated all surfaces and this result was likely to be influenced by the shape and size of the seed. The seeds are narrow, linear-wedge shaped and 4–7 mm long with a crown of numerous bristles as long as or longer than the seed. It was observed that the narrow, heavier end of the seed penetrated surfaces whilst the crown of bristles stopped at the surface and prevented the rest of the seed from penetrating further. This may indicate that for V. gracilis partial penetration is the most beneficial position for germination. Combined with its high germinability, all the above mentioned factors probably facilitate its broad distribution and suggest it is a generalist species with few small scale habitat preferences. Hawkes (2004) reported similar findings in which germination of an abundant herb species from xeric Florida shrublands was not influenced by different crust treatments (intact cyanobacterial crust, disturbed cyanobacterial crust, no crust).
Leptorhynchosscaber seeds (the smallest in the study) had high penetration on all patch types (>90%) which may be expected to enhance germination due to positioning in moister microsites. However, germination was extremely low in all treatments suggesting light and temperature conditions were not optimal, or perhaps this species has a more complex germination ecology. If germination of a species is triggered by fire or extreme temperature events, small differentiations in moisture and light availabilities on different patch types are not likely to influence germination in the absence of such triggers. Therefore, the influence of biological soil crusts in these cases is likely to be of secondary importance. Conversely, it is likely that species with high germinability but which are limited by moisture requirements will show stronger differentiation amongst patch types (due to differences in micro-environments) than those species with complex germination ecologies.
Lag times were increased on crust patches for all species, and germination time courses varied amongst patch types. Germination speed and lag, as well as the uniformity of germination, are often of more ecological significance than final percent germination (Oomes and Elberse 1976; Fowler 1988; Jurado and Westoby 1992; Kader 2005). Species that can germinate quickly in water-limited environments are likely to benefit through a competitive advantage over slower germinating seeds (Ross 1976) by taking advantage of infrequent rainfall events. However, a site suitable for seed germination is not always suitable for successful seedling establishment and growth (Schupp 1995; Li et al. 2005). Although moss and foliose lichen crusts may delay or slow rates of germination (seen in four of the five species in the present study), they could enhance survival of established seedlings relative to disturbed soil. This is particularly true for late-successional species and where abiotic stress is high (Bowker 2007). Therefore, the benefits of germinating early in the season on disturbed soil could be equalled or outweighed by enhancement of seedling survival on soil crusts. In other areas, researchers have found that once plants become established, the effect of biological soil crusts on survival and biomass appears universally positive (Li et al. 2005).
In the present study, the effect of short-turf moss, foliose lichen, litter and disturbed soil crust on germination was studied and related to seed position within the surface layer because it likely influences moisture and light conditions (Van Tooren 1990; Facelli and Pickett 1991; Serpe et al. 2006). However, effects of biological soil crusts can also be mediated by chemical factors (Zaady et al. 1997; Sedia and Ehrenfeld 2003; Hawkes 2004). Lichens in particular, have been shown to reduce germination through the synthesis of secondary compounds with allelopathic properties (Rundel 1978, although see Deines et al. 2007), and the litter of some plant species can do the same (Facelli and Pickett 1991). With respect to mosses, the effects of chemical factors on seed germination are poorly understood (van Tooren 1990). Some experiments suggest, however, that mosses do not affect germination through chemical compounds. Sedia and Ehrenfeld (2003) applied moss extracts to seeds, but this treatment did not have any effect on germination. It is also likely that variations in chemical characteristics exist between different moss species. Whether chemical factors were partly responsible for the reduction of Austrodanthonia sp. germination on short-turf moss remains unknown. The reduced penetration on this patch type suggests, however, a physical effect relating to seed water status. The fact that moss, litter and foliose lichen patches generally only delayed germination, rather than prevented it all together, suggests differences are a result of physical factors and subsequent changes to moisture and light conditions in the microsites surrounding seeds as they move through the surface layers, rather than inhibitory chemical compounds. Moreover, final percent germination and germination time courses were not significantly different between litter and foliose lichen patches for any species, suggesting they have similar ecological roles and act merely as a physical barrier to large seeded species.
In summary, this study showed that patch types differentially influence germination, and that initially, biological soil crusts may act as physical barrier to microsites more suitable for germination. Distinct differences in surface morphology of the patch types result in different seedbed environments, explaining why differences in germination exist within species. In addition, the result also appears to be influenced by biological characteristics of the seeds such as size, structure and germinability. The present study investigated effects in an artificial environment with consistent water applications. Under natural conditions, germination on the different patch types is likely to be effected by factors not considered here. In the study area, evaporation exceeds rainfall in all months except June and July (Skene and Harford 1964). As a result, moisture availability for plant growth is frequently low, particularly in warmer months. Consequently, germination is almost certainly lower than under the germination chamber conditions. Moreover, this reduction in germination in the field may be more pronounced in certain patch types. Similarly, seed predation rates may be different on bare soil than on litter or crusts (Van Tooren 1988) which could affect seed density, distribution and germination. We show, however, that the outcomes of biological soil crusts on plant germination depend on both seed characteristics and soil patch type and it is likely that these interactions have important effects on patterns of local species coexistence.
References
Baskin CC, Baskin JM (eds) (1998) Seeds. Ecology, biogeography, and evolution of dormancy and germination. Academic Press, New York
Belnap J (2002) Nitrogen fixation in biological soil crusts from southeast Utah, USA. Biol Fertility Soils 35:128–135
Belnap J (2003) The world at your feet: desert biological soil crusts. Frontiers Ecol Environ 1:181–189
Belnap J, Harper KT (1995) Influence of cryptobiotic soil crusts on elemental content of tissue of two desert seed plants. Arid Soil Res Rehabil 9:107–115
Belnap J, Prasse R, Harper KT (2001) Influence of biological soil crusts on soil environments and vascular plants. In: Belnap J, Lange OL (eds) Biological soil crusts: structure, function and management. Springer, Berlin, pp 315–326
Bliss LC, Gold WG (1999) Vascular plant reproduction, establishment, and growth and the effects of cryptogamic crusts within a polar desert ecosystem, Devon Island, N.W.T., Canada. Can J Bot 77:623–636
Bowker MA (2007) Biological soil crust rehabilitation in theory and practice: an underexploited opportunity. Restor Ecol 15:13–23
Briggs A, Morgan JW (2008) Morphological diversity and abundance of biological soil crusts differ in relation to landscape setting and vegetation type. Aust J Bot 56:246–253
Cohen J (1988) In: Statistical power analysis for the behavioural sciences NJ Hillsdale (ed). Lawrence Earlbaum Associates, Hillsdale, NJ
Deines L, Rosentreter R, Eldridge DJ, Serpe MD (2007) Germination and seedling establishment of two annual grasses on lichen-dominated biological soil crusts. Plant Soil 295:23–35
Eckert REJ, Peterson FF, Meurisse MS, Stephens JL (1986) Effects of soil-surface morphology on emergence and survival of seedlings in Big Sagebrush communities. J Range Manage 39:414–420
Eldridge DJ (1993) Cryptogams, vascular plants and soil hydrological relations: some preliminary results from the semi-arid woodlands of eastern Australia. Great Basin Nat 53:48–58
Eldridge DJ (1996) Distribution and floristics of terricolous lichens in soil crusts in arid and semi-arid New South Wales, Australia. Aust J Bot 44:581–599
Eldridge DJ, Greene RSB (1994) Microbiotic soil crusts: a review of their roles in soil and ecological processes in the rangelands of Australia. Aust J Soil Res 32:389–415
Eldridge DJ, Rosentreter R (1999) Morphological groups: a framework for monitoring microphytic crusts in arid landscapes. J Arid Environ 41:11–25
Eldridge DJ, Semple WS, Koen TB (2000) Dynamics of cryptogamic soil crusts in a derived grassland in south-eastern Australia. Aust Ecol 25:232–240
Escudero A, Martinez I, Cruz A, Otalora MAG, Maestre FT (2007) Soil lichens have species-specific effects on the seedling emergence of three gypsophile plant species. J Arid Environ 70:18–28
Facelli JM, Pickett STA (1991) Plant litter: its dynamics and effects on plant community structure. Bot Rev 57:1–28
Fowler NL (1988) What is a safe site?: neighbour, litter, germination date, and patch effects. Ecology 69:947–961
Hawkes CV (2004) Effects of biological soil crusts on seed germination of four endangered herbs in a xeric Florida shrubland during drought. Plant Ecol 170:121–134
Hawkes CV, Menges ES (2003) Effects of lichens on seedling emergence in a xeric Florida shrubland. Southeastern Nat 2:223–234
Johansen JR (1993) Cryptogamic crusts of semiarid and arid lands of North America. J Phycol 29:140–147
Jurado E, Westoby M (1992) Germination biology of selected central Australian plants. Aust J Ecol 17:341–348
Kader MA (2005) A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Proc R Soc NSW 138:65–75
Keizer PJ, van Tooren BF, During HJ (1985) Effects of bryophytes on seedling emergence and establishment of short-lived forbs in chalk grassland. J Ecol 73:493–504
Lesica P, Shelly JS (1992) Effects of cryptogamic soil crust on the population dynamics of Arabis fecunda (Brassicaceae). Am Midl Nat 128:53–60
Li X-R, Jia X-H, Long L-Q, Zerbe S (2005) Effects of biological soil crusts on seed bank, germination and establishment of two annual plant species in the Tengger Desert (N China). Plant Soil 277:375–385
Martinez I, Escudero A, Maestre FT, de la Cruz A, Guerrero C, Rubio A (2006) Small-scale patterns of abundance of mosses and lichens forming biological soil crusts in two semi-arid gypsum environments. Aust J Bot 54:339–348
Morgan JW (2006) Bryophyte mats inhibit germination of non-native species in burnt temperate native grassland remnants. Biol Invasions 8:159–168
Oomes MJM, Elberse WT (1976) Germination of six grassland herbs in microsites with different water contents. J Ecol 64:745–755
Prasse R, Bornkamm R (2000) Effect of microbiotic soil surface crusts on emergence of vascular plants. Plant Ecol 150:65–75
Pyke DA, Thompson JN (1986) Statistical analysis of survival and removal rate experiments. Ecology 67:240–245
Pyrke A (1994) Soil disturbance by native mammals and the germination and establishment of plant species. PhD dissertation, University of Tasmania
Quinn GP, Keough MJ (2002) Experimental design and data analysis for biologists. Cambridge University Press, Cambridge
Rivera-Aguilar V, Godinez-Alvarez H, Manuell-Cacheux I, Rodriguez-Zaragoza S (2005) Physical effects of biological soil crusts on seed germination of two desert plants under laboratory conditions. J Arid Environ 63:344–352
Rokich DP, Bell DT (1995) Light quality and intensity effects on the germination of species from the Jarrah (Eucalyptus marginata) forest of Western Australia. Aust J Bot 43:169–179
Ross MA (1976) Effects of temperature on germination and early growth of three plant species indigenous to central Australia. Aust J Ecol 1:259–263
Rundel PW (1978) The ecological role of secondary lichen substances. Biochem Syst Ecol 6:157–170
Schupp EW (1995) Seed-seedling conflicts, habitat choice, and patterns of plant recruitment. Am J Bot 82:399–409
Sedia EG, Ehrenfeld JG (2003) Lichens and mosses promote alternate stable plant communities in the New Jersey Pinelands. Oikos 100:447–458
Serpe M, Orm J, Barkes T, Rosentreter R (2006) Germination and seed water status of four grasses on moss-dominated biological soil crusts from arid lands. Plant Ecol 185:163–178
Skene JKM, Harford LB (1964) Soils and land use in the Rochester and Echuca districts, Victoria, including the Rochester irrigation area, Campaspe irrigation district, and Echuca east irrigation settlement. Department of Agriculture, Melbourne
St Clair LL, Webb BL, Johansen JR, Nebeker JT (1984) Cryptogamic soil crusts: enhancement of seedling establishment in disturbed and undisturbed areas. Reclam Reveg Res 3:129–136
Su YG, Li XR, Cheng YW, Tan HG, Jia RL (2007) Effects of biological soil crusts on emergence of desert vascular plants in North China. Plant Ecol 191:11–19
Thompson WA, Eldridge DJ, Bonser SP (2006) Structure of biological soil crust communities in Callitris glaucophylla woodlands of New South Wales, Australia. J Veg Sci 17:271–280
Van Tooren BF (1988) The fate of seeds after dispersal in chalk grassland: the role of the bryophyte layer. Oikos 53:41–48
Van Tooren BF (1990) Effects of a bryophyte layer on the emergence of seedlings of chalk grassland species. Acta Oecol 11:155–163
Van Tooren JB, During HJ, Lensink MJG (1985) The influence of the bryophyte layer on the microclimate in chalk grasslands. Abstr Bot 9:219–230
West NE (1990) Structure and function of microphytic soil crusts in wildland ecosystems of arid to semi-arid regions. Adv Ecol Res 20:179–223
Zaady E, Gutterman Y, Boeken B (1997) The germination of mucilaginous seeds of Plantago coronopus, Reboudia pinnata and Carrichtera annua on cyanobacterial soil crust from the Negev Desert. Plant Soil 190:247–252
Zamfir M (2000) Effects of bryophytes and lichens on seedling emergence of alvar plants: evidence from greenhouse experiments. Oikos 88:603–611
Acknowledgements
Bryan Roberts, Jane Currie, Paula Kalinowski, Katie Corbett, Ben Fallon, Brad McPhee, Jess Reifschneider and Emily Box assisted with experiment preparation and watering treatments. Pete Green, Bob Parsons, Matthew Bowker and David Eldridge improved the manuscript with helpful comments. Biological soil crust samples were collected under a research permit issued from the Department of Sustainability and Environment.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Briggs, A.L., Morgan, J.W. Seed characteristics and soil surface patch type interact to affect germination of semi-arid woodland species. Plant Ecol 212, 91–103 (2011). https://doi.org/10.1007/s11258-010-9806-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11258-010-9806-x