Abstract
We investigated the possibility of producing the therapeutic recombinant cytokine, Interferon-gamma (IFN-γ), in transgenic rice cell (Oryza sativa, cultivar TNG67) suspension cultures. We tested expression of two vector constructs, each harboring an αAmy3 leader peptide and a C-terminus His 6 tag fused to a human IFN-γ cDNA, one driven by a sucrose-starvation inducible promoter (rice αAmy3 promoter) and the other by a constitutive maize ubiquitin promoter, in rice cell suspensions, introduced via Agrobacterium tumefaciens. There was a significant difference in the amounts of recombinant IFN-γ protein produced by the Ups and Amy cell lines, as cytosolic and secretory proteins respectively. Immunological analysis of IFN-γ recombinant protein conferred a dose-dependent anti-dengue virus activity in human A549 cells, similar to the commercial product. We discuss the attractive attributes of using rice cell suspension system for the expression of therapeutic recombinant IFN-γ.
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Chen, TL., Lin, YL., Lee, YL. et al. Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures. Transgenic Res 13, 499–510 (2004). https://doi.org/10.1007/s11248-004-2376-8
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DOI: https://doi.org/10.1007/s11248-004-2376-8