Abstract
Using in vitro-grown needles of Sequoia sempervirens (D. Don.) Endl., direct shoot organogenesis was induced. The effects of three genotypes and two cytokinins, N6-benzyladenine (BA) and N-benzyl-9 (2-tetrahydropyranl) adenine (BPA), in combination with 2,4-D were investigated. Among tested cytokinins, BPA produced the highest frequency of shoot organogenesis from all three genotypes tested. Adventitious shoots were induced directly from explants without intervening callus within 5 weeks following incubation. Shoots were elongated on a 1/2 Wolter and Skoog (WS) medium supplemented with activated charcoal but without growth regulators. Later, elongated shoots were transferred to a 1/4 WS medium, but without activated charcoal and free of plant growth regulators to promote continued shoot growth. These shoots rooted spontaneously.
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Abbreviations
- BA:
-
N6-benzyladenine
- BPA:
-
N-benzyl-9 (2-tetrahydropyranl) adenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- WS:
-
Wolter and Skoog (1966) medium
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Sul, IW., Korban, S.S. Direct shoot organogenesis from needles of three genotypes of Sequoia sempervirens. Plant Cell Tiss Organ Cult 80, 353–358 (2005). https://doi.org/10.1007/s11240-004-1365-1
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DOI: https://doi.org/10.1007/s11240-004-1365-1