Abstract
Cytochrome c553 of Heliobacterium modesticaldum is the donor to P800 +, the primary electron donor of the heliobacterial reaction center (HbRC). It is a membrane-anchored 14-kDa cytochrome that accomplishes electron transfer from the cytochrome bc complex to the HbRC. The petJ gene encoding cyt c 553 was cloned and expressed in Escherichia coli with a hexahistidine tag replacing the lipid attachment site to create a soluble donor that could be made in a preparative scale. The recombinant cytochrome had spectral characteristics typical of a c-type cytochrome, including an asymmetric α-band, and a slightly red-shifted Soret band when reduced. The EPR spectrum of the oxidized protein was characteristic of a low-spin cytochrome. The midpoint potential of the recombinant cytochrome was +217 ± 10 mV. The interaction between soluble recombinant cytochrome c 553 and the HbRC was also studied. Re-reduction of photooxidized P800 + was accelerated by addition of reduced cytochrome c 553. The kinetics were characteristic of a bimolecular reaction with a second order rate of 1.53 × 104 M−1 s−1 at room temperature. The rate manifested a steep temperature dependence, with a calculated activation energy of 91 kJ mol−1, similar to that of the native protein in Heliobacillus gestii cells. These data demonstrate that the recombinant soluble cytochrome is comparable to the native protein, and likely lacks a discrete electrostatic binding site on the HbRC.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Heliobacteria are phototrophic Firmicutes with a homodimeric type 1 (FeS-type) reaction center (RC). They do not have a peripheral antennae system, and are unique in that they use bacteriochlorophyll g as the major pigment in their RC. The heliobacterial RC (HbRC) shares similarities with the RC of green sulfur bacteria and photosystem I of higher plants. The cofactor arrangement in these RCs are thought to be similar, although the core polypeptides do not share high levels of sequence identity beyond their topological arrangement in the membrane (Hauska et al. 2001; Oh-oka 2007). Heliobacteria are strict anaerobes (Madigan and Ormerod 1995), like many of the bacteria in the order Clostridiales, and can grow in the dark fermentatively. They are not autotrophic and use simple organic acids like pyruvate as carbon and electron sources.
Cytochromes are a diverse family of proteins involved in electron transfer, and are made by all eukaryotes and most prokaryotes. Typically, these proteins are associated with protein complexes bound to membranes. Among the Heliobacteria, the major membrane-anchored cytochromes have similar primary structures with high homology in the functional domain. These membrane-anchored cytochromes are monoheme c-type cytochromes, and are fully functional in isolated membranes. In the reduced form, they have an α-band centered at 553 nm, as is typical for c-type cytochromes. Although it is attached to the membrane, the primary structure of cyt c 553 gave no indications of transmembrane helices. Instead, mass spectroscopy data indicated an N-terminal modification at Cys23, where a diglyceride is attached via a thioether linkage (Albert et al. 1998). The heme-binding motif is on the C-terminus (Albert et al. 1998), separated from the N-terminus by a ~40-residue Pro/Ala-rich sequence likely to be unstructured. The stoichiometry of cyt c 553 to HbRC was measured to be around 5–6 per RC in Heliobacillus mobilis (Nitschke et al. 1995). The isolated RC from Heliobacterium modesticaldum contains no bound cytochrome (Sarrou et al. 2012).
The cyt c 553 serves as the direct reductant of P800 + in whole cells and membranes of Heliobacterium gestii, with a redox potential of +215 mV (Albert et al. 1998), only slightly more negative than the calculated redox potential of +240 mV in P800 + in H. mobilis (Nitschke et al. 1995). In whole cells of Heliobacterium chlorum, the electron transfer rate from cyt c 553 to P800 + is about 300 μs, while in membranes it is considerably slower at ~3 ms (Prince et al. 1985). Electron transfer from cyt c 553 to P800 + in membranes showed a strong dependence on both temperature and divalent cations. This is consistent with the anchoring of cyt c 553 in the membrane and collisional mode of action.
A system was developed to express cyt c 553 from H. modesticaldum in E. coli cells. The gene was restructured to remove the lipid attachment site and replace it with a hexahistidine tag to create a fully soluble electron donor to P800 +. The recombinant product was characterized in detail to ensure it retained the properties of the native protein. These data show that the expressed cyt c 553 is on par with the native cytochrome and is functional for future studies.
Experimental procedures
Construction of recombinant His6-tagged cyt c 553
The recombinant cytochrome c 553 was designed by taking the predicted mature gene product and replacing the diacylglycerol attachment site (Cys23) with a hexahistidine tag. The OmpA signal-sequence (MKKTAIAIAVALAGFATVAQA) was placed upstream of the hexahistidine tag. Cleavage by signal peptidase would result in a polypeptide with an amino terminus of AHHHHHHSSSS rather than (diacylglycerol)-CSSSS in the native protein (see Fig. 1). The synthesized sequence (Genscript) was cloned into pET30a (Novagen) using the Nde1 and Xho1 restriction sites, which were introduced at the 5′ and 3′ ends of the synthesized DNA, respectively. The resulting plasmid, called pET30a-petJ, replaces the multicloning site of pET30a with the synthetic H6-petJ gene, putting it under control of the T7 promoter.
Strain construction and protein expression
The pET30a-petJ and pRGK333 (Feissner et al. 2006) plasmids were co-transformed into BL21(DE3) cells (Invitrogen) and grown on Luria–Bertani (LB) plates supplemented with 100 mg L−1 ampicillin (Sigma-Aldrich) and 75 mg L−1 kanamycin (Fisher Scientific). A single colony was picked to inoculate a 50-mL starter culture. Cells were grown aerobically in LB media (Difco) containing 100 mg L−1 ampicillin and 75 mg L−1 kanamycin by shaking at 250 rpm at 37 °C. The overnight culture was diluted 1:100 into 4 L of LB the following morning. Cells were grown to an OD600 of 0.5. At this point, δ-aminolevulinic acid (δ-ALA; Goldbio) was added to 1 mM to support heme synthesis, and isopropyl β-D-1-thiogalactopyranoside (IPTG) was added at 300 μM to induce synthesis of both H6-PetJ and the cytochrome maturation machinery. After 3 h, cells were harvested by centrifugation for 10 min at 5,000×g, and the cell pellet was stored at −20 °C.
Purification of cyt c 553 and HbRC
Cells were thawed on ice and resuspended with 2 pellet volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl, pH 7.4) and sonicated with a Microson XL 2000 on ice for 5 rounds of 1 min at 50 % power with 30 s between each round. The cell lysate was centrifuged at 25,000×g for 30 min at 4 °C, and the supernatant was re-spun under the same conditions. The resulting supernatant was passed through a 0.22-μm filter. Clarified extract from 4 L of culture was loaded onto a column containing 1.5 mL His60 Ni Superflow resin (Clontech), which was then washed with 20 volumes of wash buffer, followed by 20 volumes of wash buffer + 3 mM imidazole. The cytochrome was eluted with 10 column volumes of wash buffer + 300 mM imidazole. The red fraction was collected and concentrated with VivaSpin20 5-kDa molecular weight cut-off centrifugal filters (Sartorius) followed by buffer exchange in the centrifugal filters with 50 mM MOPS (pH 7.0).
The HbRC from H. modesticaldum was purified as described (Sarrou et al. 2012). The native cytochrome c 553 was collected as the flow-through fraction from the carboxymethyl-Sepharose ion-exchange column during purification of the HbRC from H. modesticaldum, followed by concentration in 5-kDa molecular weight cut-off centrifugal filters.
Mass spectrometry
Native and recombinant cytochrome c 553 (both 200 μm) were diluted 1:10 in saturated sinapinic acid. Mass spectra were recorded on an Applied Biosystems DE-STR MALDI-TOF operating on electron reflector mode at 25,000 V and a laser rep rate of 20 Hz.
UV–Vis spectroscopy
The UV–Vis reduced-minus-oxidized difference spectrum of recombinant cyt c 553 was collected by subtracting the spectrum of fully oxidized protein (purified and stored in air) from that of a fully reduced sample (after addition of excess sodium dithionite). Spectra were collected on a Perkin Elmer Lamba35 spectrometer.
Electrophoresis and staining for protein or heme
A 20 % BisTris-SDS–polyacrylamide gel was loaded with solubilized native and recombinant cyt c 553 samples corresponding to 125 ng of heme (calculated from spectra of reduced samples). The gels were run at 175 V and rinsed in de-ionized water for 5 min. For heme staining, the gel was soaked in 12.5 % tricholoroacetic acid for 30 min, washed in de-ionized water for 30 min, and then soaked in a solution of 4 mM o-dianisidine, 10 % acetic acid, 10 % 0.5 M sodium citrate (pH 4.4), and 0.1 % hydrogen peroxide. After development of heme-containing polypeptides, the gel was washed in de-ionized water and photographed. For silver staining of polypeptides, the protocol of (Morrissey 1981) was used.
Redox titration of recombinant cyt c 553
Recombinant cytochrome c 553 at 200 μM in a buffer containing 50 mM MOPS (pH 7.0) and 150 mM NaCl was allowed to become fully reduced by exposure to an atmosphere of ~3 % H2 in N2 for several days at room temperature. The redox potential of the sample was monitored with a digital auto-range multi-meter (VWR) with flexible platinum and silver electrodes as the anode and cathode, respectively. The solution was slowly rendered more oxidizing by addition of small amounts of potassium ferricyanide while monitoring the potential and taking the spectrum after the potential had stabilized following each addition (The use of mediators led to technical difficulties with our voltage measurements. Extra care was taken with the addition of potassium ferricyanide). The amount of reduced cytochrome c 553 was estimated by the absorption of the α-band at 553 nm (Each spectrum was fitted as a weighted sum of the spectra of fully oxidized and fully reduced cyt c 553 in order to calculate the reduced/oxidized ratio).
Transient absorption spectroscopy
Transient absorbance spectroscopy was performed with a JTS-10 transient spectrometer (Bio-Logic). Purified HbRC samples were diluted to 0.5 μM in a buffer containing 50 mM MOPS (pH 7.0), 5 mM MgSO4, 10 mM ascorbate, 5 mM benzyl viologen (BV), and 0.03 % n-dodecyl β-D-maltoside, and were loaded into a sealed anaerobic cuvette (1-cm pathlength). A baseline was taken for 1.2 s before excitation and linear regression was used to subtract any baseline shift. A frequency-doubled Nd:YAG laser (Surlite Mini-Lite, Continuum) delivered a 6-ns saturating flash (20 mJ at 532 nm) to generate the P800 + cation. The redox state of the special pair was probed with 10-μs LED flashes centered at 803 nm, commencing 350 μs after the laser flash. Placing a 532-nm notch filter and a 780-nm high-pass filter in front of both the reference and measurement detectors minimized effects of the actinic flash. Recombinant cyt c 553 was added up to 108 μM. For the temperature-dependence experiments, the cuvette was equilibrated at temperatures varying from 10–40 °C for 5 min before absorption traces were collected. For each transient experiment, the decay of P800 + was fit to the sum of 3 exponential decay components, with the equation of y = A (x/t1)1 + A (x/t2)2 + A (x/t3)3 .
EPR spectroscopy
Continuous wave electron paramagnetic resonance (EPR) spectra were recorded at the EPR facility of University of Arizona. The magnetic field modulation frequency was 100 kHz with a field modulation of 2 mT peak-to-peak. The microwave power was 0.2 mW, the microwave frequency was 9.337 GHz, and the temperature was 6 K.
EPR spectra were simulated using EasySpin (v 4.5.0), a computational package developed by Stoll and Schweiger (2006) and based on Matlab (The MathWorks, Natick, MA, USA). The model used for the EPR simulations considered a single low-spin Fe3+ ion (S = 1/2). The fitting parameters were the g values (g x , g y , and g z ) and the line widths (ΔB x , ΔB y , and ΔB z ). The fitting procedure was similar to the one previously described by (Flores et al. 2007).
Results
Expression and purification of recombinant cyt c 553
The native cyt c 553 from H. gestii was found to be attached to a diacylglycerol molecule via a thioether linkage to Cys23, which becomes the N-terminal residue after translocation and cleavage of the signal peptide (Albert et al. 1998). Mass spectroscopic analysis of the cyt c 553 from H. modesticaldum was consistent with the same type of N-terminal modification (Sarrou et al. 2012). We replaced the native signal peptide and Cys23 with the signal peptide of OmpA followed by the sequence AHHHHHH. This would result in replacement of Cys23 with AH6 after signal peptide cleavage. We did not expect this change to have a large influence upon the structure of the cyt c 553, beyond rendering it soluble, as the first ~40 residues of these polypeptides are not well conserved between H. gestii and H. modesticaldum, beyond a propensity for Pro, small (Ala and Ser) and charged (Lys, Asp, Glu) residues (Fig. 1). This is consistent with the primary role of this sequence being an unstructured link between the membrane integration site at the N-terminus and the C-terminal cytochrome domain.
The synthetic gene, codon-optimized for E. coli, was expressed using the T7 promoter in BL21(DE3) cells, in which T7 RNA polymerase can be induced by addition of IPTG. In order to maximize expression of the recombinant cyt c, we co-expressed the ccmA-H genes (cytochrome biogenesis system I); (Feissner et al. 2006). H6-tagged cytochrome was purified from the clarified lysate by immobilized metal affinity chromatography (IMAC; see “Experimental procedures” section). Purified protein exhibited a 280/410 ratio of 1.5, and the calculated yield of the purification was 4.6 mg protein per liter, with an overall yield of 37 %.
UV–Vis spectroscopy
The spectrum of cyt c 553 reduced by dithionite is characterized by the appearance of an α-band peaking at 553 nm and a broad β-band centered at 524 nm (Fig. 2). The Soret band peaks at 412 nm in the oxidized state and at 417 nm in the reduced state (Online Resource 1). These properties are identical to those of cyt c 553 from H. gestii (Albert et al. 1998), and are typical for c-type cytochromes. The extinction coefficient of reduced protein at 417 nm was calculated to be 23.4 mM−1 cm−1 using the pyridine hemochrome method essentially as described by (Berry and Trumpower 1987).
Electrophoretic and mass spectrometric analyses
Recombinant and native cytochromes were loaded on an SDS-PAGE gel on the basis of equal heme (as estimated by the spectrum of reduced protein). The native and recombinant proteins had apparent molecular masses of 13 and 14 kDa, respectively. The slightly higher mobility of the native protein is probably due to its slightly shorter sequence and binding of SDS to the attached lipid moiety. The gels were stained for protein (Fig. 3a) or for heme (Fig. 3b). Their equivalent staining intensity indicates that the heme is covalently attached in the recombinant cyt c 553 with identical stoichiometry to that of the native protein (i.e., 1:1). The lack of other silver-staining bands in the recombinant protein preparation demonstrates that IMAC was sufficient to purify it to homogeneity.
The native cyt c 553 prep contained 4 additional bands in the 30–42 kDa range, which are resolved poorly in this gel; none of them stain for heme (Fig. 3b), as seen before (Sarrou et al. 2012). The prep in H. gestii had a similar cohort of additional polypeptides, and it was suggested that these formed a complex with the cytochrome (Albert et al. 1998). However, mass spectrometric analysis of these polypeptides from H. modesticaldum revealed them to be ABC transporters (Sarrou et al. 2012). Thus, they are unlikely to be involved with the function of cyt c 553, despite their tendency to co-purify with it.
Mass spectrometric analysis of the recombinant cyt c 553 was consistent with covalent attachment of heme to the polypeptide. We obtained a mass of 14,041 ± 14 Da, compared to an expected mass of 14,023 Da (Online Resource 2).
EPR spectroscopy of native and recombinant cyt c 553
The oxidized native cyt c 553 exhibited a low-temperature EPR spectrum typical of a low-spin cytochrome (Fig. 4), with g-values of 3.05, 2.235, and 1.36 (Table 1). Reduction by ascorbate eliminated this spectrum entirely. These g-values are very similar to those reported for the g x and g y values (3.048 and 2.238, respectively) for the cytochrome from H. gestii by (Albert et al. 1998), although they were unable to observe the low-field trough shown in this work. The recombinant protein behaved identically, although the g-tensor was slightly less anisotropic, with g-values of 2.97, 2.285, and 1.435 (Fig. 4; Table 1).
Redox titration of recombinant cyt c 553
A potentiometric titration was performed to determine the midpoint potential (E m) of the recombinant cyt c 553. The spectrum of the cytochrome solution was measured as the potential was varied. The potential of the sample was lowered by addition of small amounts of potassium ferricyanide and was monitored with an electrode. After the potential had stabilized, the spectrum was taken. Spectra were fit as a weighted sum of the spectra of the fully oxidized and fully reduced protein, to estimate the fraction of reduced cytochrome. When fit to the Nernst equation, the titration plot (Fig. 5) was consistent with a single titrating species with n = 1 (single electron oxidation/reduction) and E m = +217 ± 0.6 mV versus NHE. This is very similar to the value obtained for the native cyt c 553 from H. gestii (Albert et al. 1998).
Reduction of purified HbRC by recombinant cyt c 553
Transient spectroscopy was used to test the ability of the soluble recombinant cyt c 553 to function as an electron donor to the HbRC (P800 + + c red553 → P800 + c ox553 ). Mixtures containing the HbRC and cyt c 553 with ascorbate as electron donor and BV as electron acceptor were loaded anoxically into sealed cuvettes, and the oxidation state of P800 was monitored with 10–μs flashes at 803 nm. The HbRC was excited with a saturating laser flash at 532 nm, which resulted in instantaneous bleaching of P800 (within the timescale of the measurement) as P800 was photooxidized, followed by decay of the bleaching as P800 + was reduced (Fig. 6a). The decay of P800 + was fit to the sum of 3 exponential decay components. The fastest component (~15 ms) representing ~10 % of the decay is assigned to charge recombination of the P800 +F −X state (Heinnickel and Golbeck 2007; Sarrou et al. 2012), and is attributed to HbRCs from which the electron does not escape to BV. The slowest component (>3 s) is assigned to the slow reduction of P800 + by ascorbate.
After addition of cyt c 553, a component of intermediate rate appeared, which can be attributed to P800 + reduction by cyt c 553. As the concentration of cyt c 553 was increased, this component increased in amplitude at the expense of the slow component; it represented ~75 % of the decay at the highest concentrations tested. Addition of a fourth component did not increase the quality of the fit (data not shown). The calculated rate of reduction of P800 + by cyt c 553 is plotted against cyt c 553 concentration in Fig. 6b. Based on this, we estimate the second order rate for this reaction (P800 + + c red553 → P800 + c ox553 ) to be 1.53 × 104 M−1 s−1 at 22 °C.
In membranes of H. gestii, the rate of P800 + reduction by the native c 553 was strongly dependent upon temperature and Mg2+ concentration (Oh-Oka et al. 2002a). We wished to see if these were characteristic of the intrinsic HBRC:cyt c interaction or of the attachment of cyt c 553 to the membrane. The temperature dependence of P800 + reduction by c 553 was assessed by varying the temperature from 10 to 40 °C and measuring the kinetics of P800 + reduction as before. The reduction of P800 + by cyt c 553 was found to be highly temperature dependent, with a 40-fold increase in rate over the 30 °C span of measurement. The apparent activation energy calculated by an Arrhenius plot was 91 kJ mol−1 (Fig. 7). This is very similar to what was seen in membranes from H. gestii (Oh-Oka et al. 2002a). In contrast, however, the rate of P800 + reduction by the soluble recombinant c 553 was invariant over a Mg2+ concentration ranging from 5 μM to 50 mM (data not shown).
Discussion
Our analysis of the native cyt c 553 from H. modesticaldum revealed no differences between it and the protein from H. gestii in terms of heme attachment, UV–Vis spectrum, or EPR spectrum (Albert et al. 1998), confirming that the orthologs from these two species are very similar. One would expect this, given the high sequence conservation in their cytochrome domains (74 out of the 80 C-terminal residues; see Fig. 1). Based on its visible and EPR spectra, reduction potential, and other properties, the recombinant cyt c 553 is almost identical to the native proteins of H. modesticaldum and H. gestii. This confirms our hypothesis that the N-terminus would be a good place to insert the hexahistidine tag without altering the properties of the cytochrome.
We observed very few differences between the native and recombinant cyt c 553, beyond the slower rate of P800 + reduction by the recombinant protein, which was almost two orders of magnitude slower than the native cyt c 553 in membranes (Oh-Oka et al. 2002a). We attribute the higher rate to the incorporation of native cyt c 553 in membranes, and this is unsurprising, as the effective concentration of cyt c 553 in membranes would be much higher than what we can obtain with the recombinant protein in solution; in addition, it may be constrained to interact with the RC in ways that tend to be more productive for electron transfer between them. Consistent with this, we found that the solubilized native protein was also very slow at reducing the purified HbRC, although we were unable to test it at as high a concentration as we did with the recombinant protein (data not shown). Oh-Oka et al. (2002a) observed an approximate fourfold increase in the P800 + reduction rate upon addition of MgCl2 to membranes in the range of 0.3–3 mM. This effect of divalent cations on electron donation rate is somewhat similar to what had been seen with the interaction of plastocyanin and P700 + (Tamura et al. 1981). However, we saw no difference in the rate of electron donation from soluble cyt c 553 to P800 + in the range of 0.005–50 mM MgCl2 using the soluble version of cyt c 553 (data not shown). Therefore, the dependence of this rate upon divalent cations must be an effect upon membranes and unrelated to the interface between cyt c 553 and the HbRC.
The cyt c 553 of H. modesticaldum seems to fall in line with other small c-type cytochromes of similar function. Rhodobacter sphaeroides, Chlorobaculum tepidum, and H. gestii all contain c-type cytochromes that donate to their respective RCs. These cytochrome all have low-spin hemes (Drepper and Mathis 1997; Azai et al. 2010; Albert et al. 1998) and share similar spectral characteristics, including an asymmetric asymmetric α-band, and a slightly red-shifted Soret band when reduced (Oh-oka et al. 1998; Albert et al. 1998; Dutton et al. 1975); Fig. 2). They also have similar midpoint potentials, all being within the range of +170 to +220 mV. Thus, the cyt c 553 of H. modesticaldum seems very much like the other heliobacterial c-type cytochromes, which are themselves fairly typical members of this family. Furthermore, the EPR parameters obtained in this work for both native and recombinant cyt c 553 of H. modesticaldum suggest that they belong to the family of low-spin ferriheme proteins containing two histidines as axial ligands (Walker et al. 1984). The small differences in the observed g-values (Table 1) likely reflect slightly different orientations of such ligands.
The interaction of cyt c 553 with the HbRC of H. modesticaldum is very similar to that seen in other type-I photosynthetic RC systems. Interestingly, the cytochromes in Chlorobium and Heliobacteria have midpoints that give a relatively small gap in potential when compared to the special pair of the corresponding organism. The gap in C. tepidum may be as small as 45 mV (Okumura et al. 1994), while it is 55 and 50 mV in H. chlorum (Prince et al. 1985) and H. mobilis (Nitschke et al. 1995), respectively. Given the highly conserved nature of the photosynthetic proteins within Heliobacteria, it is likely that the gaps in potential are similar for H. modesticaldum and H. gestii. Even the donor proteins for photosystem I, plastocyanin and cyt c 6, typically have a gap of less than 100 mV with respect to the special pair (Nakamura et al. 2011). These ranges likely indicate the minimum driving force for an electron transfer rate that does not limit overall electron flow, while optimizing energy conservation. The activation energies of electron transfer from the c-type cytochrome to its native RC are also similar, with E a = 88 kJ mol−1 for H. gestii (Oh-Oka et al. 2002a) and Ea = 101 kJ mol−1 for C. tepidum (Oh-oka et al. 1997) in their native context. We determined an activation energy of 91 kJ mol−1 when using the soluble version of H. modesticaldum c 553 (Fig. 7).
The theoretical isoelectric points were calculated from the functional portion of the mature protein (the signal sequence and transmembrane helices were omitted to approximate the functional portion of the protein). In addition, the cysteine residues involved in heme binding were replaced in the calculations by glutamic acids to more appropriately simulate the propionates on the heme. The fully matured recombinant cyt c 553 has a calculated pI of 7.8. The cyt c 553 proteins from different heliobacteria are: H. gestii (7.1), H. modesticaldum (native) (7.3), and H. mobillis (5.9). The soluble cytochrome from R. sphaeroides has a calculated pI of 6.1, while the cytochrome of the green sulfur bacterium C. tepidum (pI = 9.4) has a somewhat more basic isoelectric point, perhaps indicating a larger dependence on electrostatic interactions for optimal cooperation with the acceptor. In both the chlorobial and purple bacterial systems, the cytochrome is pre-bound to the RC, while in the heliobacterial system the cytochrome seems to depend on a collisional mode for electron transfer.
The rate of electron transfer from cyt c 553 to P800 + is ~3 ms in membranes from H. gestii (Oh-Oka et al. 2002a). This is much slower than that seen with R. sphaeroides (20–40 μs) (Dutton et al. 1975) or C. tepidum (110 μs) (Oh-oka et al. 1995). Although the rate is increased when the reaction is measured in whole cells of H. gestii (0.3 ms) (Oh-oka et al. 2002b) or H. modesticaldum (0.5 ms) (Redding et al. 2013), the order of magnitude difference is maintained when compared to the electron transfer kinetics of C. tepidum (7 μs) (Hauska et al. 2001), and R. sphaeroides (1 μs) (Wang et al. 1994). The lack of membrane tether in the recombinant cytochrome is likely the dominant factor influencing the disparity in electron transfer rate when compared to the native cytochrome. Water-soluble cytochromes likely have a stronger dependence on electrostatic interactions to orient correctly from a longer range, as they are faster to associate and slower to dissociate. This was strikingly demonstrated in a study comparing the ability of the soluble cyt c 2, membrane-attached cyt c y , and a mutant of cyt c y in which the membrane tether was removed, to support respiratory or photosynthetic electron transfer in Rhodobacter capsulatus (Ozturk et al. 2008). Similar to our in vitro results here, removal of the membrane-spanning helix from cyt c y resulted in slowed electron transfer to the RC in cells, likely due to a drop in the rate of binding. When tethered to the membrane, cyt c 553 is limited to 2-dimensional movement and does not require long-range electrostatic interactions to bind the HbRC, which allows it to facilitate electron throughput despite the lack of a tight-binding interaction.
References
Albert I, Rutherford AW, Grav H, Kellermann J, Michel H (1998) The 18 kDa cytochrome c553 from Heliobacterium gestii: gene sequence and characterization of the mature protein. Biochemistry 37(25):9001–9008. doi:10.1021/bi9731347
Azai C, Tsukatani Y, Itoh S, Oh-oka H (2010) C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria. Photosynth Res 104(2–3):189–199. doi:10.1007/s11120-009-9521-4
Berry EA, Trumpower BL (1987) Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra. Anal Biochem 161(1):1–15
Drepper F, Mathis P (1997) Structure and function of cytochrome c2 in electron transfer complexes with the photosynthetic reaction center of Rhodobacter sphaeroides: optical linear dichroism and EPR. Biochem 36(6):1428–1440. doi:10.1021/bi961351m
Dutton PL, Petty KM, Bonner HS, Morse SD (1975) Cytochrome c2 and reaction center of Rhodospeudomonas spheroides Ga. membranes. Extinction coefficients, content, half-reduction potentials, kinetics and electric field alterations. Biochim Biophys Acta 387(3):536–556
Feissner RE, Richard-Fogal CL, Frawley ER, Loughman JA, Earley KW, Kranz RG (2006) Recombinant cytochromes c biogenesis systems I and II and analysis of haem delivery pathways in Escherichia coli. Mol Microbiol 60(3):563–577. doi:10.1111/j.1365-2958.2006.05132.x
Flores M, Isaacson R, Abresch E, Calvo R, Lubitz W, Feher G (2007) Protein-cofactor interactions in bacterial reaction centers from Rhodobacter sphaeroides R-26: II. Geometry of the hydrogen bonds to the primary quinone Q(A)(-) by H-1 and H-2 ENDOR spectroscopy. Biophys J 92(2):671–682. doi:10.1529/Biophysj.106.092460
Hauska G, Schoedl T, Remigy H, Tsiotis G (2001) The reaction center of green sulfur bacteria. Biochim Biophys Acta 1507(1–3):260–277
Heinnickel M, Golbeck JH (2007) Heliobacterial photosynthesis. Photosynth Res 92(1):35–53
Madigan MT, Ormerod JG (1995) Taxonomy, physiology and ecology of heliobacteria. In: Blankenship RE, Madigan MT, Bauer CE (eds) Anoxygenic photosynthetic bacteria. Kluwer Academic Publishers, Dordrecht, pp 17–30
Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117(2):307–310
Nakamura A, Suzawa T, Kato Y, Watanabe T (2011) Species dependence of the redox potential of the primary electron donor p700 in photosystem I of oxygenic photosynthetic organisms revealed by spectroelectrochemistry. Plant Cell Physiol 52(5):815–823. doi:10.1093/pcp/pcr034
Nitschke W, Liebl U, Matsuura K, Kramer DM (1995) Membrane-bound c-type cytochromes in Heliobacillus mobilis. In vivo study of the hemes involved in electron donation to the photosynthetic reaction center. Biochem 34(37):11831–11839
Oh-oka H (2007) Type 1 reaction center of photosynthetic heliobacteria. Photochem Photobiol 83(1):177–186. doi:10.1562/2006-03-29-IR-860
Oh-oka H, Kamei S, Matsubara H, Iwaki M, Itoh S (1995) Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green sulfur bacterium, Chlorobium tepidum. FEBS Lett 365(1):30–34
Oh-oka H, Iwaki M, Itoh S (1997) Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of the green sulfur bacterium Chlorobium tepidum. Biochem 36(30):9267–9272
Oh-oka H, Iwaki M, Itoh S (1998) Membrane-bound cytochrome c z couples quinol oxidoreductase to the P840 reaction center complex in isolated membranes of the green sulfur bacterium Chlorobium tepidum. Biochem 37(35):12293–12300
Oh-Oka H, Iwaki M, Itoh S (2002a) Electron donation from membrane-bound cytochrome c to the photosynthetic reaction center in whole cells and isolated membranes of Heliobacterium gestii. Photosynth Res 71(1–2):137–147. doi:10.1023/A:1014911832504
Oh-oka H, Iwaki M, Itoh S (2002b) Electron donation from membrane-bound cytochrome c to the photosynthetic reaction center in whole cells and isolated membranes of Heliobacterium gestii. Photosyn Res 71(1–2):137–147
Okumura N, Shimada K, Matsuura K (1994) Photo-oxidation of membrane-bound and soluble cytochrome c in the green sulfur bacterium Chlorobium tepidum. Photosynth Res 41(1):125–134
Ozturk Y, Lee DW, Mandaci S, Osyczka A, Prince RC, Daldal F (2008) Soluble variants of Rhodobacter capsulatus membrane-anchored cytochrome cy are efficient photosynthetic electron carriers. J Biol Chem 283(20):13964–13972. doi:10.1074/jbc.M800090200
Prince RC, Gest H, Blankenship RE (1985) Thermodynamic properties of the photochemical reaction center of Heliobacterium chlorum. Biochimica et Biophysica Acta (BBA)—Bioenergetics 182:345–349
Redding KE, Sarrou I, Rappaport F, Santabarbara S, Lin S, Reifschneider KT (2013) Modulation of the fluorescence yield in heliobacterial cells by induction of charge recombination in the photosynthetic reaction center. Photosynth Res. doi:10.1007/s11120-013-9957-4
Sarrou I, Khan Z, Cowgill J, Lin S, Brune D, Romberger S, Golbeck JH, Redding KE (2012) Purification of the photosynthetic reaction center from Heliobacterium modesticaldum. Photosynth Res 111(3):291–302. doi:10.1007/s11120-012-9726-9
Stoll S, Schweiger A (2006) EasySpin, a comprehensive software package for spectral simulation and analysis in EPR. J Magn Reson 178(1):42–55. doi:10.1016/J.Jmr.08.013
Tamura N, Itoh S, Yamamoto Y, Nishimura M (1981) Electrostatic interaction between plastocyanin and P700 in the electron-transfer reaction of photosystem I-enriched particles. Plant Cell Physiol 22(4):603–612
Walker FA, Reis D, Balke VL (1984) Models of the cytochromes-B.5. Electron-paramagnetic-res studies of low-spin iron(III) tetraphenylporphyrins. J Am Chem Soc 106(23):6888–6898. doi:10.1021/Ja00335a003
Wang S, Li X, Williams JC, Allen JP, Mathis P (1994) Interaction between cytochrome c2 and reaction centers from purple bacteria. Biochem 33(27):8306–8312
Acknowledgments
This work was funded by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy through grant DE-SC0010575 to KER. We thank Andrei Astashkin (Univ. of Arizona) for assistance with the EPR measurements and Patricia Baker for assistance with growing heliobacteria.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Kashey, T.S., Cowgill, J.B., McConnell, M.D. et al. Expression and characterization of cytochrome c 553 from Heliobacterium modesticaldum . Photosynth Res 120, 291–299 (2014). https://doi.org/10.1007/s11120-014-9982-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11120-014-9982-y