Abstract
The Arabidopsis (Arabidopsis thaliana) genome contains 15 genes encoding protein homologs of the barley mildew resistance locus o (MLO) protein biochemically shown to have a seven-transmembrane domain topology and localize to the plasma membrane. Towards elucidating the functions of MLOs, the largest family of seven-transmembrane domain proteins specific to plants, we comprehensively determined AtMLO gene expression patterns by a combination of experimental and in silico studies. Experimentation comprised analyses of transgenic Arabidopsis lines bearing promoter::β-glucuronidase (GUS) transcriptional fusions as well as semi-quantitative determination of transcripts by reverse transcription coupled to polymerase chain reaction (RT-PCR). These results were combined with information extracted from public gene profiling databases, and compared to the expression patterns of genes encoding the heterotrimeric G-protein subunits. We found that each AtMLO gene has a unique expression pattern and is regulated differently by a variety of biotic and/or abiotic stimuli, suggesting that AtMLO proteins function in diverse developmental and response processes. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s).
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Abbreviations
- AGB1:
-
the Arabidopsis Gβ subunit
- AGG1:
-
the Arabidopsis Gγ subunit1
- AGG2:
-
the Arabidopsis Gγ subunit2
- FRET:
-
Förster/Fluorescence Resonance Energy Transfer
- GCR1:
-
G-Coupled Receptor1
- GPA1:
-
the Arabidopsis Gα subunit
- GPCRs:
-
G-protein coupled receptors
- GUS:
-
β-glucuronidase
- MLO:
-
mildew resistance locus o
- RGS1:
-
Regulator of G-protein Signaling
- 7TM:
-
seven-transmembrane
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Chen, Z., Hartmann, H.A., Wu, MJ. et al. Expression analysis of the AtMLO Gene Family Encoding Plant-Specific Seven-Transmembrane Domain Proteins. Plant Mol Biol 60, 583–597 (2006). https://doi.org/10.1007/s11103-005-5082-x
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DOI: https://doi.org/10.1007/s11103-005-5082-x