Abstract
Cotton fibres are single, highly elongated cells derived from the outer epidermis of ovules, and are developmentally similar to the trichomes of Arabidopsis thaliana. To identify genes involved in the molecular control of cotton fibre initiation, we isolated four putative homologues of the Arabidopsis trichome-associated gene TRANSPARENT TESTA GLABRA1 (TTG1). All four WD-repeat genes are derived from the ancestral D diploid genome of tetraploid cotton and are expressed in many tissues throughout the plant, including ovules and growing fibres. Two of the cotton genes were able to restore trichome formation in ttg1 mutant Arabidopsis plants. Both these genes also complemented the anthocyanin defect in a white-flowered Matthiola incana ttg1 mutant. These results demonstrate parallels in differentiation between trichomes in cotton and Arabidopsis, and indicate that these cotton genes may be functional homologues of AtTTG1.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
S.F. Altschul W. Gish W. Miller E.W. Myers D.J. Lipman (1990) ArticleTitleBasic local alignment search tool J. Mol. Biol. 215 403–410
A.S. Basra C.P. Malik (1984) ArticleTitleDevelopment of the cotton fibre Int. Rev. Cytol. 89 65–113
C.A. Beasley I.P. Ting (1974) ArticleTitleEffects of plant growth substances on in vitro fibre development from unfertilised cotton ovules Am. J. Bot. 61 188–194
J.E. Burn U.A. Hurley R.J. Birch T. Arioli A. Cork R.E. Williamson (2002) ArticleTitleThe cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control Plant J. 32 949–960
C.C. Carey J.T. Strahle D.A. Selinger V.L. Chandler (2004) ArticleTitleMutations in the pale aleurone color1 regulatory gene of the Zea mays anthocyanin pathway have distinct phenotypes relative to the functionally similar TRANSPARENT TESTA GLABRA1 gene in Arabidopsis thaliana Plant Cell 16 450–464
A. Casas-Mollano N. Destefano-Beltrán (2000) ArticleTitleCharacterisation of a cDNA encoding a TTG1-like protein (Accession No AF220203) from Malus domestica fruits Plant Physiol. 122 1458
S.J. Clough A.F. Bent (1998) ArticleTitleFloral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant J. 16 735–743
N. Vetten Particlede F. Quattrocchio J. Mol R. Koes (1997) ArticleTitleThe an11locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals Genes Dev. 11 1422–1434
B. Epping M. Kittel B. Ruhnau V. Hemleben (1990) ArticleTitleIsolation and sequence analysis of a chalcone synthase cDNA of Matthiola incana R Br. (Brassicaceae). Plant Mol. Biol. 14 1061–1063
T. Franks D.G. He M. Thomas (1998) ArticleTitleRegeneration of transgenic Vitis vinifera L Sultana plants: genotypic and phenotypic analysis. Mol. Breed. 4 321–333
M.E. Galway J.D. Masucci A.M. Lloyd V. Walbot R.W. Davis J.W. Schiefelbein (1994) ArticleTitleThe TTG gene is required to specify epidermal cell fate and cell patterning in the Arabidopsis root Dev. Biol. 166 740–754
A.P. Gleave (1992) ArticleTitleA versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome Plant Mol. Biol. 20 1203–1207
D.A. Graves J.M. Stewart (1988) ArticleTitleChronology of the differentiation of cotton (Gossypium hirsutum L.) fibre cells Planta 175 254–258
S.E. Harmer S.J. Orford J.N. Timmis (2002) ArticleTitleCharacterisation of six α-expansin genes in Gossypium hirsutum (upland cotton) Mol. Gen. Genom. 268 1–9
C.P. Hodgson R.Z. Fisk (1987) ArticleTitleHybridisation probe size control: optimised ‘oligolabelling’ Nucl. Acids Res. 15 6295
C.Y. Hsu R.G. Creech J.N. Jenkins D.P. Ma (1999) ArticleTitleAnalysis of promoter activity of cotton lipid transfer protein gene LTP6 in transgenic tobacco plants Plant Sci. 143 63–70
C. Jiang R. Wright K. El-Zik A.H. Paterson (1988) ArticleTitlePolyploid formation created unique avenues for response to selection in Gossypium (cotton) Proc. Natl. Acad. Sci. USA 95 4419–4424
H. Kappert (1949) ArticleTitleDie genetik das incana-charakters und der anthocyanbildung bei der levkoje Zuchter 19 289–297
M. Koornneef (1981) ArticleTitleThe complex syndrome of ttg mutants Arabidopsis Inform. Serv. 18 45–51
J.C. Larkin D.G. Oppenheimer A.M. Lloyd E.T. Paparozzi M.D. Marks (1994) ArticleTitleRoles of the GLABROUS1 and TRANSPARENT TESTA GLABRA genes in Arabidopsis trichome development Plant Cell 6 1065–1076
J.C. Larkin N. Young M. Prigge M.D. Marks (1996) ArticleTitleThe control of trichome number and spacing in Arabidopsis Development 122 997–1005
G.R. Lazo P.A. Stein R.A. Ludwig (1991) ArticleTitleA DNA transformation-competent Arabidopsis genomic library in Agrobacterium Biotech. 9 963–967
D. Li R. Roberts (2001) ArticleTitleWD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases Cell Mol. Life Sci. 58 2085–2097
H.-C. Liu R.G. Creech J.N. Jenkins D.-P. Ma (2000) ArticleTitleCloning and promoter analysis of the cotton lipid transfer protein gene Ltp3 Biochim. Biophys. Acta 1487 106–111
E.J. Neer C.J. Schmidt R. Nambudripad T.F. Smith (1994) ArticleTitleThe ancient regulatory-protein family of WD-repeat proteins Nature 371 297–300 Occurrence Handle10.1038/371297a0 Occurrence Handle1:CAS:528:DyaK2cXmvFGgtb0%3D Occurrence Handle8090199
D.G. Oppenheimer P.L. Herman S. Sivakumaran J. Esch M.D. Marks (1991) ArticleTitleA myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules Cell 67 483–493 Occurrence Handle10.1016/0092-8674(91)90523-2
S.J. Orford J.N. Timmis (1997) ArticleTitleAbundant mRNAs specific to the developing cotton fibre Theor. Appl. Genet. 94 909–918
S.J. Orford J.N. Timmis (2000) ArticleTitleExpression of a lipid transfer protein gene family during cotton fibre development Biochim. Biophys. Acta 1483 275–284
T. Payne J. Clement D. Arnold A. Lloyd (1999) ArticleTitleHeterologous myb genes distinct from GL1 enhance trichome production when overexpressed in Nicotiana tabacum Development 126 671–682
C.T. Payne F. Zhang A.M. Lloyd (2000) ArticleTitleGL3 encodes a bHLH protein that regulates trichome development in Arabidopsis through interaction with GL1 and TTG1 Genetics 156 1349–1362
W.R. Pearson D.J. Lipman (1988) ArticleTitleImproved tools for biological sequence comparison Proc. Natl. Acad. Sci. USA 85 2444–2448
N.A. Ramsay A.R. Walker M. Mooney J.C. Gray (2003) ArticleTitleTwo basic-helix–loop–helix genes (MYC-146 and GL3) from Arabidopsis can activate anthocyanin biosynthesis in a white-flowered Matthiola incana mutant Plant Mol. Biol. 52 679–688
J. Sambrook E. Fritsch T. Maniatis (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbour Laboratory Press Cold Spring Harbor, NY, USA
S. Schellmann A. Schnittger V. Kirik T. Wada K. Okada A. Beermann J. Thumfarht G. Jürgens M. Hülskamp (2002) ArticleTitleTRYPTYCHONand CAPRICEmediate lateral inhibition during trichome and root hair patterning in Arabidopsis EMBO J. 21 5036–5046
Y. Shimizu S. Aotsuka O. Hasegawa T. Kawada T. Sakuno F. Sakai T. Hayashi (1997) ArticleTitleChanges in levels of mRNAs for cell wall-related enzymes in growing cotton fibre cells Plant Cell Physiol. 38 375–378
P.S. Soltis D.E. Soltis M.W. Chase (1999) ArticleTitleAngiosperm phylogeny inferred from multiple genes as a tool for comparative biology Nature 402 402–404 Occurrence Handle10.1038/46528 Occurrence Handle1:CAS:528:DyaK1MXnvVykurg%3D Occurrence Handle10586878
K. Sompornpailin Y. Makita M. Yamazaki K. Saito (2002) ArticleTitleA WD-repeat-containing putative regulatory protein in anthocyanin biosynthesis in Perilla frutescens Plant Mol. Biol. 50 485–495
J.M. Stewart (1975) ArticleTitleFibre initiation on the cotton ovule (Gossypium hirsutum) Am. J. Bot. 62 723–730
D.B. Szymanski A.M. Lloyd M.D. Marks (2000) ArticleTitleProgress in the molecular genetic analysis of trichome initiation and morphogenesis in Arabidopsis Trends Plant Sci. 5 214–219
P. Vain N. Keen J. Murillo C. Rathus C. Nemes J.J. Finer (1993) ArticleTitleDevelopment of the Particle Inflow Gun Plant Cell Tissue Organ Cult. 33 237–246
J. Van’t Hof (1999) ArticleTitleIncreased nuclear DNA content in developing cotton fibre cells Am. J. Bot. 86 776–779
A.R. Walker M.D. Marks (2000) ArticleTitleTrichome initiation in Arabidopsis Adv. Bot. Res. 31 219–236
A.R. Walker P.A. Davison A.C. Bolognesi-Winfield C.M. James N. Srinivasan T.L. Blundell J.J. Esch M.D. Marks J.C. Gray (1999) ArticleTitleThe TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein Plant Cell 11 1337–1349 Occurrence Handle10.1105/tpc.11.7.1337
C.R. Walkerpeach J. Velten (1994) Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems S.B. Gelvin R.A. Schilperoort (Eds) Plant Molecular Biology Manual Kluwer Academic Publishers Belgium 1–19
J.F. Wendel (1989) ArticleTitleNew World tetraploid cottons contain Old World cytoplasm Proc. Natl. Acad. Sci. USA 86 4132–4136
T.A. Wilkins J.A. Jernstedt (1999) Molecular genetics of developing cotton fibres A.S. Basra (Eds) Cotton Fibres: developmental Biology, Quality Improvement, and Textile Processing. The Haworth Press, Inc. USA 47–63
T.A. Wilkins K. Rajasekaran D.M. Anderson (2000) ArticleTitleCotton biotechnology Crit. Rev. Plant Sci. 19 511–550
L.H. Yu C. Gaitatzes E. Neer T.F. Smith (2000) ArticleTitleThirty-plus functional families from a single motif Prot. Sci. 9 2470–2476
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Humphries, J.A., Walker, A.R., Timmis, J.N. et al. Two WD-repeat genes from cotton are functional homologues of the Arabidopsis thaliana TRANSPARENT TESTA GLABRA1 (TTG1) gene. Plant Mol Biol 57, 67–81 (2005). https://doi.org/10.1007/s11103-004-6768-1
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s11103-004-6768-1