Abstract
A simple method of a bicolor (multicolor), fast-Fourier, PAM chlorophyll fluorometry has been developed to obtain fluorescence induction curves. Quantum yields of PSII photochemistry were determined with blue and red simultaneously applied pulsed measuring lights for three subsequent 20-min periods of dark-, light-adaptation under actinic light and dark recovery. Measuring lights were cross-combined with blue and red actinic lights and saturation pulses. Coefficients of chromatic divergence were calculated as a ratio of the quantum yields obtained by red measuring light to that obtained by blue measuring light. Adaptation of Ficus benjamina and Hordeum vulgare leaves under blue (but not red) actinic light resulted in the sufficient increase of chromatic divergence. In addition, fraction of active, non(photo)inhibited, PSII centers was shown to be dependent on the color of measuring light. Thus, color of the light sources should be considered when reporting results of parameters evaluated from fluorescence induction curves.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- AB and AR:
-
blue and red actinic lights
- AL:
-
actinic light
- Ft:
-
operating or effective Chl fluorescence level in the light-adapted state
- F0 :
-
minimal Chl fluorescence level in dark-adapted state
- FFT:
-
fast Fourier transform
- Fm, Fm' :
-
maximal Chl fluorescence level in dark-adapted and light-adapted state
- Fm1'' and Fm2'' :
-
maximal Chl fluorescence levels recovered at the beginning and at the end of the second dark period
- Fv :
-
variable Chl fluorescence
- LED:
-
light-emitting diode
- MB and MR:
-
blue and red measuring flashes
- ML:
-
measuring light
- SP:
-
saturating pulse
- PAM:
-
pulse amplitude modulation (fluorometry)
- SB and SR:
-
blue and red saturating pulses
- ξ:
-
chromatic divergence of quantum yields of PSII photochemistry
References
Baránková B., Lazár D., Nauš J.: Analysis of the effect of chloroplast arrangement on optical properties of green tobacco leaves.–Remote Sens. Environ. 174: 181–196, 2016.
Figueroa F.L., Jerez C.G., Korbee N.: Use of in vivo chlorophyll fluorescence to estimate photosynthetic activity and biomass productivity in microalgae grown in different culture systems.–Lat. Am. J. Aquat. Res. 41: 801–819, 2013.
Genty B., Briantais J.-M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.–Biochim. Biophys. Acta 990: 87–92, 1989.
Guo Z, Wang F, Xiang X. et al.: Systemic induction of photosynthesis via illumination of the shoot apex is mediated sequentially by Phytochrome B, auxin and hydrogen peroxide in tomato.–Plant Physiol. 172: 1259–1272, 2016.
Hogewoning S.W., Trouwborst G., Maljaars H. et al.: Blue light dose-responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light.–J. Exp. Bot. 61: 3107–3117, 2010.
Hogewoning S.W., Wientjes E., Douwstra P. et al.: Photosynthetic quantum yield dynamics: from photosystems to leaves.–Plant Cell 24: 1921–1935, 2012.
Jakob T., Schreiber U., Kirchesch V. et al.: Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits.–Photosynth. Res. 83: 343–361, 2005.
Kitajima M., Butker W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone.–Biochim. Biophys. Acta 376: 105–115, 1975.
Lazár D.: Parameters of photosynthetic energy partitioning.–J. Plant Physiol. 175: 131–147, 2015.
Lysenko V., Varduny T.: Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?–Sci. Rep. 3: 3373, 2013.
Lysenko V.S., Varduny T.V., Simonovich E.I. et al.: Far-red spectrum of second Emerson effect: a study using dualwavelength pulse amplitude modulation fluorometry.–Am. J. Biochem. Biotech. 10: 234–240, 2014.
Muneer S., Kim E.J., Park J.S. et al.: Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.).–Int. J. Mol. Sci. 15: 4657–4670, 2014.
Pfündel E.E.: Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of FV/FM on excitation wavelength.–Photosynth. Res. 100: 163–177, 2009.
Pfündel E.E., Latuche G., Meister A., Cerovic Z.G.: Linking chloroplast relocation to different responses of photosynthesis to blue and red radiation in low and high light-acclimated leaves of Arabidopsis thaliana.–Photosynth. Res. 137: 105–128, 2018.
Schreiber U., Klughammer C., Kolbowski J.: Assessment of wavelength-dependent parameters of photosynthetic electron transport with a new type of multi-color PAM chlorophyll fluorometer.–Photosynth. Res. 113: 127–144, 2012.
Schreiber U.: Pulse-amplitude-modulation (PAM) fluorometry and saturation pulse method: An overview.–In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: a Signature of Photosynthesis. Pp. 279–319. Springer, Dordrecht 2004.
Schreiber U., Klughammer C.: Wavelength-dependent photodamage to Chlorella investigated with a new type of multicolor PAM chlorophyll fluorometer.–Photosynth. Res. 114: 165–177, 2013.
Suggett D.J., Goyen S., Evenhuis C. et al.: Functional diversity of photobiological traits within the genus Symbiodinium appears to be governed by the interaction of cell size with cladal designation.–New Phytol. 208: 370–381, 2015.
Szabó M., Wangpraseurt D., Tamburic B. et al.: Effective light absorption and absolute electron transport rates in the coral Pocillopora damicornis.–Plant Physiol. Bioch. 83: 159–167, 2014.
Yu X., Liu H., Klejnot J. et al.: The cryptochrome blue light receptors.–Arabidopsis Book 8: e0135, 2010.
Author information
Authors and Affiliations
Corresponding author
Additional information
Acknowledgements: V.L. and T.V. were supported by Ministry of Education and Science of Russian Federatian by grant BCh0110- 11/2017-25 and D.L. was supported by grant LO1204 from the National Program of Sustainability I, Ministry of Education, Youth and Sports, Czech Republic. The laboratory experiments and measurements were supported by grant BCh0110-11/2017-25 and performed with the equipment of Multiaccess center “Biotechnology, Biomedicine and Environmental Monitoring”, Laboratory of Plant Physiology and Ecology of Academy of Biology and Biotechnology of Southern Federal University.
Rights and permissions
About this article
Cite this article
Lysenko, V., Lazár, D. & Varduny, T. A method of a bicolor fast-Fourier pulse-amplitude modulation chlorophyll fluorometry. Photosynthetica 56, 1447–1452 (2018). https://doi.org/10.1007/s11099-018-0848-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11099-018-0848-y