Abstract
We report here the screening of sixteen cyanobacterial and three green algal strains from Thailand for their potential biohydrogen production. Five filamentous cyanobacterial species, namely Calothrix elenkinii, Fischerella muscicola, Nostoc calcicola, Scytonema bohneri, and Tolypothrix distorta, all possessing nitrogenase activity, showed potentially high biohydrogen production. These five strains showed higher hydrogen production in the absence than in the presence of nitrogen. In particular, F. muscicola had a 17-fold increased hydrogen production under combined nitrogen and sulfur deprived conditions. Among various sugars as a carbon source, glucose at 0.1% (w/v) gave the maximal hydrogen production of 10.9 μmol(H2) mg–1(Chl) h–1 in T. distorta grown in BG11 medium without nitrate. Increasing light intensity up to 250 μmol(photon) m–2 s–1 increased hydrogen production in F. muscicola and T. distorta. Overall results indicate that both F. muscicola and T. distorta have a high potential for hydrogen production amenable for further improvement by using molecular genetics technique.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Chl:
-
chlorophyll
- hox gene:
-
bidirectional hydrogenase gene
- hup gene:
-
uptake hydrogenase gene
- PCC:
-
Pasteur Culture Collection
- TISTR:
-
Thailand Institute of Scientific and Technological Research
References
Allahverdiyeva Y., Leino H., Saari L. et al.: Screening for biohydrogen production by cyanobacteria isolated from the Baltic Sea and Finnish lakes.–Int. J. Hydrogen Energ. 35: 1117–1127, 2010.
Antal T.K., Lindblad P.: Production of H2 by sulphur-deprived cells of the unicellular cyanobacteria Gloeocapsa alpicola and Synechocystis sp. PCC 6803 during dark incubation with methane at various extracellular pH.–J. Appl. Microbiol. 98: 114–120, 2005.
Aoyama K., Uemura I., Miyake J. et al.: Fermentative metabolism to produce hydrogen gas and organic compounds in a cyanobacterium, Spirulina platensis.–J. Ferment. Bioeng. 83: 17–20, 1997.
Baebprasert W., Lindblad P., Incharoensakdi A.: Response of H2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp. strain PCC6803.–Int. J. Hydrogen Energ. 35: 6611–6616, 2010.
Berberoǧlu H., Jay J., Pilon L.: Effect of nutrient media on photobiological hydrogen production by Anabaena variabilis ATCC29413.–Int. J. Hydrogen Energ. 33: 1172–1184, 2008.
Bothe H., Schmitz O., Yates M.G. et al.: Nitrogen fixation and hydrogen metabolism in cyanobacteria.–Microbiol. Mol. Biol. Rev. 74: 529–551, 2010.
Chen P.C., Fan S.H., Chiang C.L. et al.: Effect of growth conditions on the hydrogen production with cyanobacterium Anabaena sp. strain CH3.–Int. J. Hydrogen Energ. 33: 1460–1464, 20
Dutta D., De D., Chaudhuri S., Bhattacharya S.K.: Hydrogen production by cyanobacteria.–Microb. Cell Fact. 4: 36, 2005
Fay P.: Oxygen relations of nitrogen fixation in cyanobacteria.–Microbiol. Rev. 56: 340–373, 1992.
Fouchard S., Hemschemeier A., Caruana A. et al.: Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived Chlamydomonas cells.–Appl. Environ. Microbiol. 71: 6199–6205, 2005.
Gutekunst K., Chen Xi, Schreiber K. et al.: The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotropic nitrate-limiting conditions–J. Biol. Chem. 289: 1930–1937, 2014.
Hansel A., Lindblad P.: Towards optimization of cyanobacteria as biotechnologically relevant producers of molecular hydrogen, a clean and renewable energy source.–Appl. Microbiol. Biot. 50: 153–160, 1998.
Khetkorn W., Lindblad P., Incharoensakdi A.: Enhanced biohydrogen production by the N2-fixing cyanobacterium Anabaena siamensis strain TISTR 8012.–Int. J. Hydrogen Energ. 35: 12767–12776, 2010.
Khetkorn W., Lindblad P., Incharoensakdi A.: Inactivation of uptake hydrogenase leads to enhanced and sustained hydrogen production with high nitrogenase activity under high light exposure in the cyanobacterium Anabaena siamensis TISTR 8012.–J. Biol. Eng. 6: 19, 2012.
Khetkorn W., Rastogi R.P., Incharoensakdi A. et al.: Microalgal hydrogen production–a review.–Bioresour. Technol. 243: 1194–1206, 2017.
MacKinney G.: Absorption of light by chlorophyll solutions.–J. Biol.Chem. 140: 315–322, 1941.
Maneeruttanarungroj C., Lindblad P., Incharoensakdi A.: A newly isolated green alga, Tetraspora sp. CU2551, from Thailand with efficient hydrogen production.–Int. J. Hydrogen Energ. 35: 13193–13199, 2010.
Masukawa H., Nakamura K., Mochimaru M. et al.: Photobiological hydrogen production and nitrogenase activity in some heterocystous cyanobacteria.–Biohydrogen 2: 63–66, 2001.
Masukawa H., Mochimaru M, Sakurai H.: Disruption of the uptake hydrogenase gene, but not of the bidirectional hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium Anabaena sp. 7120.–Appl. Microbiol. Biot. 58: 618–624, 2002.
Melis A., Zhang L.P., Forestier M. et al.: Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii.–Plant Physiol. 122: 127–136, 20
Møller K.T., Jensen T.R., Akiba E. et al.: Hydrogen–A sustainable energy carrier.–Prog. Nat. Sci. 27: 34–40, 2017.
Park J.I., Lee J., Sim S.J. et al.: Production of hydrogen from marine macro-algae biomass using anaerobic sewage sludge microflora.–Biotechnol. Bioproc. E. 14: 307–315, 2009.
Patel S., Madamwar D.: Photohydrogen production from a coupled system of Halobacterium halobium and Phormidium valderianum.–Int. J. Hydrogen Energ. 19: 733–738, 1994.
Raksajit W., Satchasataporn K., Lehto K. et al: A. Enhancement of hydrogen production by the filamentous non-heterocystous cyanobacterium Arthrospira sp. PCC 8005.–Int. J. Hydrogen Energ. 37: 18791–18797, 2012.
Reddy P.M., Spiller H., Albrecht S.L. et al.: Photodissimilation of fructose to H2 and CO2 by a dinitrogen fixing cyanobacterium, Anabaena variabilis.–Appl. Environ. Microb. 62: 1220–1226, 1996.
Stanier R.Y., Kunisawa R., Mandel M. et al.: Purification and properties of unicellular blue-green algae (order Chroococcales).–Bacteriol. Rev. 35: 171–205, 1971.
Tamagnini P., Axelsson R., Lindberg P. et al.: Hydrogenase and hydrogen metabolism of cyanobacteria.–Microbiol. Mol. Biol. Rev. 66: 1–20, 2002.
Tamagnini P., Leitão E., Oliveira P. et al.: Cyanobacterial hydrogenase: diversity, regulation and applications.–FEMS Microbiol. Rev. 31: 692–720, 2007.
Tsygankov A.A., Kosourov S.N., Tolstygina I.V. et al.: Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions.–Int. J. Hydrogen Energ. 31: 1574–1584, 2006.
Yeager C.M., Milliken C.E., Bagwell C.E. et al.: Evaluation of experimental conditions that influence hydrogen production among heterocystous cyanobacteria.–Int. J. Hydrogen Energ. 36: 7487–7499, 2011.
Yoshino F., Ikeda H., Masukawa H. et al.: High photobiological hydrogen production activity of a Nostoc sp. PCC 7422 uptake hydrogenase-deficient mutant with high nitrogenase activity.–J. Mar. Biotechnol. 9: 101–112, 2007.
Author information
Authors and Affiliations
Corresponding author
Additional information
Acknowledgements: This work was supported by the Commission for Higher Education (CHE), Thailand (the university staff development consortium), the Frontier Research Project on Energy Cluster of Chulalongkorn University (CU-59-048-EN), and the Thailand Research Fund (IRG5780008) including the travel grant from CU Rachadapiseksompote Endowment Fund to AI. PY thanks Centre for International Mobility for CIMO scholarship, the Graduate School of Chulalongkorn University for Post-doctoral Fellowship. PY and WR thank Erasmus Mundus Action II scholarship (EXPERTS4Asia and EXPERTS-SUSTAIN).
Rights and permissions
About this article
Cite this article
Yodsang, P., Raksajit, W., Aro, EM. et al. Factors affecting photobiological hydrogen production in five filamentous cyanobacteria from Thailand. Photosynthetica 56, 334–341 (2018). https://doi.org/10.1007/s11099-018-0789-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11099-018-0789-5