Abstract
The kinetics of bacteriochlorophyll fluorescence in intact cells of the purple nonsulfur bacterium Rhodobacter sphaeroides were measured under continuous and pulsed actinic laser diode (808 nm wavelength and maximum 2 W light power) illumination on the micro- and millisecond timescale. The fluorescence induction curve was interpreted in terms of a combination of photochemical and triplet fluorescence quenchers and was demonstrated to be a reflection of redox changes and electron carrier dynamics. By adjustment of the conditions of single and multiple turnovers of the reaction center, we obtained 11 ms–1 and 120 μs–1 for the rate constants of cytochrome c23+ detachment and cyclic electron flow, respectively. The effects of cytochrome c2 deletion and chemical treatments of the bacteria and the advantages of the fluorescence induction study on the operation of the electron transport chain in vivo were discussed.
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Abbreviations
- BChl:
-
bacteriochlorophyll
- Chl:
-
chlorophyll
- F0 :
-
minimal fluorescence yield of the dark-adapted state
- Fmax :
-
maximal fluorescence yield of the light-adapted state
- Fv :
-
variable fluorescence
References
Asztalos E., Italiano F., Milano F. et al.: Early detection of mercury contamination by fluorescence induction of photosynthetic bacteria.–Photoch. Photobio. Sci. 9: 1218–1223, 2010.
Asztalos E., Sipka G., Maróti P.: Fluorescence relaxation in intact cells of photosynthetic bacteria: donor and acceptor side limitations of reopening of the reaction center.–Photosynth. Res. 124: 31–44, 2015.
Bína D., Litvín R., Vácha F.: Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides.–Photosynth. Res. 105: 115–121, 20
Chi S.C., Mothersole D.J., Dilbeck P. et al.: Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway.–Biochim. Biophys. Acta 1847: 189–201, 2015.
Comayras F., Jungas C., Lavergne J.: Functional consequences of the organization of the photosynthetic apparatus in Rhodobacter sphaeroides. I. Quinone domains and excitation transfer in chromatophores and reaction center antenna complexes.–J. Biol. Chem. 280: 11203–11213, 2005.
Crofts A.R., Meinhardt S.W., Jones K.R., Snozzi M.: The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides: A modified Q-cycle mechanism.–Biochim. Biophys. Acta 723: 202–218, 1983.
de Rivoyre M., Ginet N., Bouyer P., Lavergne J.: Excitation transfer connectivity in different purple bacteria: a theoretical and experimental study.–Biochim. Biophys. Acta 1797: 1780–1794, 2010.
Donohue T.J., Kaplan S.: Genetic techniques in rhodospirillaceae.–Methods Enzymol. 204: 459–485, 1991.
Duysens L.N.M.: Transfer of light energy within the pigment systems present in photosynthesizing cells.–Nature 168: 548–550, 1951.
Geyer T., Helms V.: A spatial model of the chromatophore vesicles of Rhodobacter sphaeroides and the position of the cytochrome bc1 complex.–Biophys. J. 91: 921–926, 2006.
Joliot P., Joliot A., Vermeglio A.: Fast oxidation of the primary electron acceptor under anaerobic conditions requires the organization of the photosynthetic chain of Rhodobacter sphaeroides in supercomplexes.–BBA-Bioenergetics 1706: 204–214, 2005.
Kautsky H., Hirsch A.: [New experiments on carbonic acid assimilation.]–Naturwissenschaften 19: 964, 1931. [In German]
Kis M., Asztalos E., Sipka G., Maróti P.: Assembly of photosynthetic apparatus in Rhodobacter sphaeroides as revealed by functional assessments at different growth phases and in synchronized and greening cells.–Photosynth. Res. 122: 261–273, 2014.
Kis M., Sipka G., Asztalos E. et al.: Purple non-sulfur photosynthetic bacteria monitor environmental stresses.–J. Photoch. Photobio. B 151: 110–117, 2015.
Klamt S., Grammel H., Straube R. et al: Modelling the electron transport chain of purple nonsulfur bacteria.–Mol. Syst. Biol. 4: 156, 2008.
Koblizek M., Shih J.D., Breitbart S.I. et al.: Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence.–Biochim. Biophys. Acta 1706: 220–231, 2005.
Kocsis P., Asztalos E., Gingl Z., Maróti P.: Kinetic bacteriochlorophyll fluorometer.–Photosynth. Res. 105: 73–82, 2010.
Maróti P.: Kinetics and yields of bacteriochlorophyll fluorescence: redox and conformation changes in reaction center of Rhodobacter sphaeroides.–Eur. Biophys. J. 37: 1175–1184, 20
Maróti P.: Induction and relaxation of bacteriochlorophyll fluorescence in photosynthetic bacteria.–In: Pessarakli M. (ed): Handbook of Photosynthesis, 3rd ed. Pp. 463–483. CRC Press, Boca Raton–London–New York 2016.
Niederman R.A.: Development and dynamics of the photosynthetic apparatus in purple phototrophic bacteria.–Biochim. Biophys. Acta 1857: 232–246, 2016.
Sambrook J., Fritsch E.F., Maniatis T.: Molecular cloning: a laboratory manual, 2nd ed. Page A.1. Cold Spring Harbor Laboratory Press, New York 1989.
Siström W.R.: The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroides.–J. Gen. Microbiol. 28: 607–616, 1962.
Siström, W.R., Transfer of chromosomal genes mediated by plasmid r68.45 in Rhodopseudomonas sphaeroides.–J. Bacteriol. 131: 526–532, 1977
Stirbet A., Govindjee: Chlorophyll a fluorescence induction: a personal perspective of the thermal phase, the J-I-P rise.–Photosynth. Res. 113: 15–61, 2012.
Trissl H.W.: Antenna organization in purple bacteria investigated by means of fluorescence induction curves.–Photosynth. Res. 47: 175–185, 1996.
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Acknowledgements: We are indebted to Dr. Emese Asztalos for her contribution to the early phase of this work. The authors gratefully acknowledge financial support from GINOP-2.3.2-15-2016-00001, OTKA-K 112688, Photosynthesis–Life from Light–Foundation (Hungary) (G. Sipka), COST (CM1306), EFOP-3.6.2-16-2017-00001 (M. Kis and P. Maróti), the Bill and Roberta Blankenship CENS Research Endowment, the Hal and Alma Reagan Faculty Leave, and the van Dyck Faculty Leave (J.L. Smart).
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Sipka, G., Kis, M., Smart, J.L. et al. Fluorescence induction of photosynthetic bacteria. Photosynthetica 56, 125–131 (2018). https://doi.org/10.1007/s11099-017-0756-6
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DOI: https://doi.org/10.1007/s11099-017-0756-6